Specific heterologous F(ab’)2 antibodies revert blood incoagulability resulting from envenoming by Lonomia obliqua caterpillars.
Abstract: Contact with Lonomia obliqua caterpillars results in a bleeding syndrome characterized by hemorrhage and blood coagulation disturbances. Conventional therapy using antifibrinolytics or cryoprecipitates has been unable to treat pathophysiologic alterations. As antivenoms are effective therapy for treatment of victims of venomous animals, a process of manufacturing a specific antilonomic serum by immunizing horses with Lonomia caterpillar bristle extracts (LBE) was developed. Lonomia caterpillar bristle extracts exhibited several protein bands on SDS-PAGE, induced blood coagulation abnormalities and lethality in mice, and stimulated specific antibody production in horses. Sera obtained from immunized horses were rich in anti-LBE specific antibodies distributed among the horse IgG isotypes. These antibodies had the ability to recognize various LBE antigens as well as to neutralize their coagulopathy-inducing activity. The antivenom manufactured by the developed process was composed of purified and sterilized F(ab')2 with ED50 = 38.61 microl, potency = 0.29 mg/ml, and 95% confidence limit of potency 0.20-1.36.
Publication Date: 2001-07-21 PubMed ID: 11463118DOI: 10.4269/ajtmh.2001.64.283Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article is about the development of an antivenom derived from horses to treat blood coagulation disturbances caused by contact with Lonomia obliqua caterpillars.
Problem Statement
- The authors observed that contact with Lonomia obliqua caterpillars often results in a bleeding syndrome characterized by hemorrhage and disturbances in the ability of the blood to coagulate.
- Existing treatments, including antifibrinolytics or cryoprecipitates, have proven ineffective in treating these blood coagulation conditions.
Objective
- The aim of this research was to develop a specific antilonomic serum (antivenom) by immunizing horses with Lonomia caterpillar bristle extracts (LBE) to neutralize the coagulopathy-inducing activity of the caterpillar venom.
Methodology
- A process of manufacturing a specific antilonomic serum was created by immunizing horses with Lonomia caterpillar bristle extracts.
- The research further delved into the properties of LBE, elucidating that it stimulated specific antibody production in horses and induced blood coagulation abnormalities in mice. This serum was rich in anti-LBE specific antibodies.
Results
- The antibodies derived from horse sera were not only able to recognize various LBE antigens but also displayed the capability to neutralize their ability to induce coagulopathy.
- The antivenom created using this process was composed of purified and sterilized F(ab’)2, a type of antibody fragment, and was demonstrated to have an ED50 (a measure of the dose that produces an effect in 50% of the population) of 38.61 microliters, a potency of 0.29 mg/ml, and a 95% confidence limit of potency ranging from 0.20 to 1.36.
Conclusions
- The study presents a promising therapeutic solution for treating blood coagulation disturbances resulting from contact with Lonomia obliqua caterpillars.
- Through the development of a specialized antivenom consisting of specific horse-derived anti-LBE antibodies, this research might lead to effective treatment options for patients suffering from this specific type of envenoming.
Cite This Article
APA
Rocha-Campos AC, Gonçalves LR, Higashi HG, Yamagushi IK, Fernandes I, Oliveira JE, Ribela MT, Sousa-E-Silva MC, da Silva WD.
(2001).
Specific heterologous F(ab’)2 antibodies revert blood incoagulability resulting from envenoming by Lonomia obliqua caterpillars.
Am J Trop Med Hyg, 64(5-6), 283-289.
https://doi.org/10.4269/ajtmh.2001.64.283 Publication
Researcher Affiliations
- Divisão de Produção de Soros, Imunopatologia, Instituto Butantan, São Paulo, SP, Brazil. rocha_campos@hotmail.com
MeSH Terms
- Animals
- Antibody Formation
- Blood Coagulation Disorders / chemically induced
- Electrophoresis, Polyacrylamide Gel
- Immunoglobulin Fab Fragments / immunology
- Lethal Dose 50
- Mice
- Mice, Inbred BALB C
- Moths
- Neutralization Tests
- Tissue Distribution
- Venoms / immunology
- Venoms / toxicity
Citations
This article has been cited 7 times.- Alvarez-Flores MP, Gomes RN, Trevisan-Silva D, Oliveira DS, Batista IFC, Buri MV, Alvarez AM, DeOcesano-Pereira C, de Souza MM, Chudzinski-Tavassi AM. Lonomia obliqua Envenoming and Innovative Research.. Toxins (Basel) 2021 Nov 23;13(12).
- Seldeslachts A, Peigneur S, Tytgat J. Caterpillar Venom: A Health Hazard of the 21st Century.. Biomedicines 2020 May 30;8(6).
- Berger M, de Moraes JA, Beys-da-Silva WO, Santi L, Terraciano PB, Driemeier D, Cirne-Lima EO, Passos EP, Vieira MAR, Barja-Fidalgo TC, Guimarães JA. Renal and vascular effects of kallikrein inhibition in a model of Lonomia obliqua venom-induced acute kidney injury.. PLoS Negl Trop Dis 2019 Feb;13(2):e0007197.
- Sano-Martins IS, Duarte AC, Guerrero B, Moraes RHP, Barros EJG, Arocha-Piñango CL. Hemostatic disorders induced by skin contact with Lonomia obliqua (Lepidoptera, Saturniidae) caterpillars.. Rev Inst Med Trop Sao Paulo 2017 Apr 20;59:e24.
- Medeiros DN, Torres HC, Troster EJ. Accident involving a 2-year-old child and Lonomia obliqua venom: clinical and coagulation abnormalities.. Rev Bras Hematol Hemoter 2014 Nov-Dec;36(6):445-7.
- Berger M, Santi L, Beys-da-Silva WO, Oliveira FM, Caliari MV, Yates JR 3rd, Vieira MA, Guimarães JA. Mechanisms of acute kidney injury induced by experimental Lonomia obliqua envenomation.. Arch Toxicol 2015 Mar;89(3):459-83.
- Veiga AB, Ribeiro JM, Guimarães JA, Francischetti IM. A catalog for the transcripts from the venomous structures of the caterpillar Lonomia obliqua: identification of the proteins potentially involved in the coagulation disorder and hemorrhagic syndrome.. Gene 2005 Aug 1;355:11-27.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
