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Home / Equine Research Bank / Histochem Cell Biol / Specific localisation of gap junction protein connexin 32 in...
Histochemistry and cell biology2005; 125(3); 307-313; doi: 10.1007/s00418-005-0047-3

Specific localisation of gap junction protein connexin 32 in the gastric mucosa of horses.

Abstract: In the glandular stomach, gap junctional intercellular communication (GJIC) plays an important role in the gastric mucosal defense system, and loss of GJIC is associated with ulcer formation. In spite of the high incidence of gastric ulcers in horses, particularly at pars nonglandularis, the presence of gap junctions in the equine stomach has not yet been studied. The objective was to obtain basic data on the distribution of gap junction protein connexin 32 (Cx32) in the different regions of normal equine gastric mucosa. Samples of mucosa were taken from seven horses at cardiac, fundic, and pyloric region and pars nonglandularis. To detect Cx32, immunohistochemical staining and Western blot analysis were performed. Corresponding mRNA was shown by RT-PCR and localised in tissue sections by in situ hybridisation. Cx32 was found in the glandular regions, whereas it was not detectable in squamous mucosa. Within the glandular epithelium, Cx32 was abundant in surface and foveolar cells and decreased towards the proliferative zone of the glands. These results suggest that gap junctions develop during the maturation of surface cells. Whether the lack of Cx32 at pars nonglandularis contributes to its susceptibility for developing ulcers, has to be further elucidated.
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Publication Date: 2005-10-05 PubMed ID: 16205941DOI: 10.1007/s00418-005-0047-3Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the presence and distribution of a specific gap junction protein call connexin 32 (Cx32) in different regions of a horse’s stomach. It highlights that the absence of this protein might contribute to the high susceptibility of horses to develop stomach ulcers.

Objectives and Methodology

  • The study’s primary goal was to establish fundamental information regarding the distribution and presence of the protein connexin 32 (Cx32) in various parts of the horse’s gastric mucosa. Gap junctional intercellular communication plays a significant part in the defence system of the gastric mucosa, and its absence is linked with ulcer formation.
  • The researchers followed a procedural analysis involving sample collection from seven different horses. These samples were extracted from different parts of the horses’ stomachs, including the cardiac, fundic, and pyloric regions, as well as the pars nonglandularis.
  • To detect the presence of the Cx32 protein, techniques such as immunohistochemical staining, Western blot analysis, RT-PCR, and in situ hybridisation were employed. The corresponding mRNA was localised in the tissue sections by the in situ hybridisation technique.

Key Findings

  • The research found the presence of Cx32 in the glandular regions of the stomach but not in the squamous mucosa. The glandular epithelium showed an abundance of this protein, particularly in surface and foveolar cells.
  • However, the level of Cx32 decreased towards the proliferative zone of the glands. This suggests that gap junctions, which are vital for intercellular communication and tissue functioning, develop during the maturation of surface cells.

Implications and Further Research

  • The study concludes by suggesting that the lack of Cx32 protein at the pars nonglandularis could contribute to the increased propensity of that region to develop ulcers. However, this hypothesis needs to be further investigated in future studies.
  • Understanding the role of gap junctions and the distribution of Cx32 within the stomach could provide valuable insights for veterinary medicine and the treatment of gastric ulcers in horses.

Cite This Article

APA
Fink C, Hembes T, Brehm R, Weigel R, Heeb C, Pfarrer C, Bergmann M, Kressin M. (2005). Specific localisation of gap junction protein connexin 32 in the gastric mucosa of horses. Histochem Cell Biol, 125(3), 307-313. https://doi.org/10.1007/s00418-005-0047-3

Publication

Histochemistry and cell biology
ISSN: 0948-6143
NlmUniqueID: 9506663
Country: Germany
Language: English
Volume: 125
Issue: 3
Pages: 307-313

Researcher Affiliations

Fink, Cornelia
  • Department of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University Giessen, Frankfurter Strasse 98, Giessen, Germany. Cornelia.Fink@vetmed.uni-giessen.de
Hembes, Tanja
    Brehm, Ralph
      Weigel, Roswitha
        Heeb, Cornelia
          Pfarrer, Christiane
            Bergmann, Martin
              Kressin, Monika

                MeSH Terms

                • Animals
                • Base Sequence
                • Cell Communication
                • Connexins / genetics
                • Connexins / metabolism
                • DNA Primers / genetics
                • Gap Junctions / metabolism
                • Gastric Mucosa / anatomy & histology
                • Gastric Mucosa / metabolism
                • Horses / genetics
                • Horses / metabolism
                • Immunohistochemistry
                • In Situ Hybridization
                • RNA, Messenger / genetics
                • RNA, Messenger / metabolism
                • Reverse Transcriptase Polymerase Chain Reaction
                • Gap Junction beta-1 Protein

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