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Home / Equine Research Bank / Theriogenology / Sperm motility patterns and metabolism in Catalonian donkey ...
Theriogenology2005; 63(6); 1706-1716; doi: 10.1016/j.theriogenology.2004.07.022

Sperm motility patterns and metabolism in Catalonian donkey semen.

Abstract: The Sperm-Class Analyzer detected four subpopulations of spermatozoa with different motility characteristics in the ejaculate of the Catalonian donkey. Significant differences (P < 0.001) in the distribution of these subpopulations, as well as in total sperm number and percentage total motility, were seen in the diluted semen of four sampled donkeys. All the ejaculates evaluated showed excellent semen quality characteristics; the sperm they contained was more rapid than horse sperm. Principal components analysis showed sperm l-lactate production to be a good predictor of semen condition. This, plus the characteristics of the motility patterns of the different sperm subpopulations, provides an excellent overall indicator of semen quality.
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Publication Date: 2005-03-15 PubMed ID: 15763113DOI: 10.1016/j.theriogenology.2004.07.022Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the motility patterns and metabolism in Catalonian donkey semen, finding that different characteristics could provide valuable indicators of semen quality.

Research Methodology and Results

  • The research involved using a Sperm-Class Analyzer, a device that is able to differentiate and classify sperm into various subpopulations based on their motility characteristics. In this study, the device was able to identify four distinct subpopulations of spermatozoa in the ejaculate of the Catalonian donkey.
  • There were significant variations in the distribution of these different sperm subpopulations, as well as in the total sperm number and their overall motility, among the semen of four different Catalonian donkeys that were studied.
  • Interestingly, all of the ejaculates under investigation exhibited high quality semen characteristics, with the sperm demonstrating higher speed compared to horse sperm.

Main Findings and Conclusion

  • A principal components analysis, a statistical procedure used to highlight key variances in large datasets, indicated that sperm l-lactate production could be a reliable determinant of semen condition. L-lactate is a byproduct of energy metabolism in sperm, indicating their efficiency and energy use.
  • The researchers concluded that the combined information of l-lactate production and the differing motility patterns of the sperm subpopulations could serve as a comprehensive indicator of semen quality in Catalonian donkeys. This could be used as a standard measure to assess fertility in these animals, perhaps offering important information for breeding programs or fertility research.

Cite This Article

APA
Miró J, Lobo V, Quintero-Moreno A, Medrano A, Peña A, Rigau T. (2005). Sperm motility patterns and metabolism in Catalonian donkey semen. Theriogenology, 63(6), 1706-1716. https://doi.org/10.1016/j.theriogenology.2004.07.022

Publication

Theriogenology
ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 63
Issue: 6
Pages: 1706-1716

Researcher Affiliations

Miró, J
  • Unit of Reproduction, Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Spain. jordi.miro@uab.es
Lobo, V
    Quintero-Moreno, A
      Medrano, A
        Peña, A
          Rigau, T

            MeSH Terms

            • Animals
            • Computers
            • Equidae
            • Horses
            • Male
            • Semen / cytology
            • Sperm Motility
            • Spermatozoa / abnormalities
            • Spermatozoa / metabolism
            • Spermatozoa / ultrastructure

            Citations

            This article has been cited 16 times.
            1. Yánez-Ortiz I, Catalán J, Delgado-Bermúdez A, Carluccio A, Miró J, Yeste M. Addition of Reduced Glutathione (GSH) to Freezing Medium Reduces Intracellular ROS Levels in Donkey Sperm. Vet Sci 2021 Dec 2;8(12).
              doi: 10.3390/vetsci8120302pubmed: 34941829google scholar: lookup
            2. Yánez-Ortiz I, Catalán J, Mateo-Otero Y, Dordas-Perpinyà M, Gacem S, Yeste N, Bassols A, Yeste M, Miró J. Extracellular Reactive Oxygen Species (ROS) Production in Fresh Donkey Sperm Exposed to Reductive Stress, Oxidative Stress and NETosis. Antioxidants (Basel) 2021 Aug 27;10(9).
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            3. Gacem S, Valverde A, Catalán J, Yánez Ortiz I, Soler C, Miró J. A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse. Front Vet Sci 2021;8:651477.
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            4. Miró J, Catalán J, Marín H, Yánez-Ortiz I, Yeste M. Specific Seminal Plasma Fractions Are Responsible for the Modulation of Sperm-PMN Binding in the Donkey. Animals (Basel) 2021 May 13;11(5).
              doi: 10.3390/ani11051388pubmed: 34068214google scholar: lookup
            5. Martins-Bessa A, Quaresma M, Leiva B, Calado A, Navas González FJ. Bayesian Linear Regression Modelling for Sperm Quality Parameters Using Age, Body Weight, Testicular Morphometry, and Combined Biometric Indices in Donkeys. Animals (Basel) 2021 Jan 13;11(1).
              doi: 10.3390/ani11010176pubmed: 33451023google scholar: lookup
            6. Catalán J, Papas M, Trujillo-Rojas L, Blanco-Prieto O, Bonilla-Correal S, Rodríguez-Gil JE, Miró J, Yeste M. Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light. Front Cell Dev Biol 2020;8:588621.
              doi: 10.3389/fcell.2020.588621pubmed: 33365309google scholar: lookup
            7. Papas M, Catalán J, Recuero S, Morrell JM, Yeste M, Miró J. Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils. Animals (Basel) 2020 Nov 16;10(11).
              doi: 10.3390/ani10112128pubmed: 33207812google scholar: lookup
            8. Gacem S, Catalán J, Valverde A, Soler C, Miró J. Optimization of CASA-Mot Analysis of Donkey Sperm: Optimum Frame Rate and Values of Kinematic Variables for Different Counting Chamber and Fields. Animals (Basel) 2020 Oct 29;10(11).
              doi: 10.3390/ani10111993pubmed: 33138237google scholar: lookup
            9. Catalán J, Papas M, Gacem S, Mateo-Otero Y, Rodríguez-Gil JE, Miró J, Yeste M. Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure. Biology (Basel) 2020 Aug 29;9(9).
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            10. Miró J, Marín H, Catalán J, Papas M, Gacem S, Yeste M. Seminal Plasma, Sperm Concentration, and Sperm-PMN Interaction in the Donkey: An In Vitro Model to Study Endometrial Inflammation at Post-Insemination. Int J Mol Sci 2020 May 14;21(10).
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            14. Terrell KA, Wildt DE, Anthony NM, Bavister BD, Leibo SP, Penfold LM, Marker LL, Crosier AE. Evidence for compromised metabolic function and limited glucose uptake in spermatozoa from the teratospermic domestic cat (Felis catus) and cheetah (Acinonyx jubatus). Biol Reprod 2010 Nov;83(5):833-41.
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            15. Rodríguez Gil JE, Blanco-Prieto O. Techniques to Determine Mammalian Sperm Capacitation. Methods Mol Biol 2025;2897:463-495.
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            16. Ren H, Wen X, He Q, Yi M, Dugarjaviin M, Bou G. Comparative Study on the Sperm Proteomes of Horses and Donkeys. Animals (Basel) 2024 Jul 31;14(15).
              doi: 10.3390/ani14152237pubmed: 39123763google scholar: lookup
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