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Home / Equine Research Bank / Methods Mol Biol / Sperm Vitrification in Horse and Donkey.
Methods in molecular biology (Clifton, N.J.)2025; 2897; 137-145; doi: 10.1007/978-1-0716-4406-5_9

Sperm Vitrification in Horse and Donkey.

Abstract: Sperm vitrification is a novel-assisted reproductive technique that is increasingly gaining relevance in the last years. This technique allows to cryopreserve sperm from valuable stallions and donkeys without the exposure to permeable cryoprotectants, particularly toxic for the gametes of these species.This chapter aims to describe the current range of methodologies available that are key to ensure sperm quality after vitrification and warming of stallion and donkey sperm.
© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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Publication Date: 2025-04-09 PubMed ID: 40202632PubMed Central: 6284284DOI: 10.1007/978-1-0716-4406-5_9Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates a new method for preserving the sperm of valuable stallions and donkeys called sperm vitrification. This method avoids exposing sperm to harmful cryoprotectants typically used in freezing.

Research Focus

  • This study concentrates on sperm vitrification, a relatively new technique for the reproductive assistance of horses and donkeys. The researchers emphasize the need for such a method due to the particularly negative effects of conventional cryoprotectants on the gametes of these animals.

Importance of Sperm Vitrification

  • Sperm vitrification is an innovative and significant process because it presents an alternative to the conventional procedure of cryopreserving sperm. Traditional methods often expose the sperm to toxic cryoprotectants that can have detrimental effects on their quality.

Objective of the Research

  • The principal goal of the research is to map out the range of methodologies currently available to ensure the quality of stallion and donkey sperm after undergoing vitrification and thawing processes. In detailing these methodologies, the authors hope to influence the further exploration and execution of sperm vitrification in practical applications.

Impact of the Study

  • The research’s implications stretch beyond the stallions and donkeys in their study. If the proposed methodologies prove reliably successful, they could potentially influence how cryopreservation is conducted in other species, thereby reducing the potentially harmful exposure to toxic cryoprotectants.

Cite This Article

APA
Hidalgo M, Ortiz I. (2025). Sperm Vitrification in Horse and Donkey. Methods Mol Biol, 2897, 137-145. https://doi.org/10.1007/978-1-0716-4406-5_9

Publication

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
NlmUniqueID: 9214969
Country: United States
Language: English
Volume: 2897
Pages: 137-145

Researcher Affiliations

Hidalgo, Manuel
  • Department of Animal Medicine and Surgery, University of Córdoba, Córdoba, Spain.
Ortiz, Isabel
  • Department of Animal Medicine and Surgery, University of Córdoba, Córdoba, Spain. iortiz@uco.es.

MeSH Terms

  • Animals
  • Equidae
  • Horses
  • Male
  • Cryopreservation / methods
  • Cryopreservation / veterinary
  • Vitrification
  • Spermatozoa / cytology
  • Semen Preservation / methods
  • Semen Preservation / veterinary
  • Cryoprotective Agents / pharmacology

References

This article includes 28 references
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Citations

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