Stability and reproducibility of ADVIA 120-measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin content (CH(R)) in the diagnosis of iron deficiency.
Abstract: Modern laser-based haematology analysers such as the ADVIA 120 have species-specific software and offer the possibility of assessing new haematological parameters. These parameters have yet to be evaluated, and as these analysers are often used in referral laboratories, it is important to know whether the values of haematological parameters change during sample transport. Therefore, samples of EDTA-anticoagulated blood from nine healthy dogs and EDTA- and citrate-anticoagulated blood from six healthy horses were collected and stored at room temperature for 72 and 48 hours, respectively. In canine samples, WBC and the red blood cell parameters Hb, Hb(cell), Ht, MCV, and MCHC changed significantly after only 24 hours of storage. Thus if canine blood samples need to be stored for 24 hours or longer, Hb, RBC, and MCH would appear to be more reliable parameters than Ht, Hb(cell), MCV, and MCHC. The cytoplasmic haemoglobin content (CH(R)) remained stable up to 48 hours. Both dog and horse platelet numbers were stable over time when blood was anticoagulated with EDTA. Of the platelet-derived parameters, MPC was already significantly lower 2 hours after collection of equine blood samples and was also significantly lower 24 hours after collection of canine blood samples. In contrast, MPV levels were significantly higher 48 hours after sample collection. Initial platelet numbers and platelet parameters were significantly different in citrate-anticoagulated blood and EDTA-anticoagulated blood, and platelet numbers and MPM decreased significantly in citrate-anticoagulated blood samples after only 4 hours of storage. After reference intervals for CH(R) had been established using samples from 53 non-anaemic dogs and 150 non-anaemic cats, the use of CH(R) to detect iron deficiency anaemia was tested in 63 dogs and 55 cats with different diseases. With the help of ROC curves, the optimal cut-off point was determined to be 1.22 fmol in dogs and 0.88 fmol in cats, resulting in a sensitivity of 95.2% and a specificity of 90.5% in dogs and 93.8% and 76.9% in cats, respectively.
Publication Date: 2009-05-13 PubMed ID: 19431960
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- Journal Article
Summary
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This research explores the stability and reproducibility of red blood cell and platelet parameters measured by ADVIA 120 in dogs, cats, and horses. It also investigates the use of reticulocyte haemoglobin content (CHr) in diagnosing iron deficiency.
Methodology
- The study used samples of EDTA-anticoagulated blood from nine healthy dogs and both EDTA- and citrate-anticoagulated blood from six healthy horses.
- These samples were stored at room temperature for 72 and 48 hours respectively to assess the changes in the haematological parameters over time.
Results
- Various changes in blood parameters were observed in canine samples over a 24 hour period, suggesting some measurements, such as Hb (hemoglobin), RBC (red blood cells), and MCH (mean corpuscular hemoglobin), are more reliable for these samples than others like Hb(cell), MCV (mean corpuscular volume), and MCHC (mean corpuscular hemoglobin concentration).
- The cytoplasmic hemoglobin content (CHr) was found to remain stable up to 48 hours.
- The study found that platelet numbers in dogs and horses remained stable over time when blood was anticoagulated with EDTA (Ethylenediaminetetraacetic Acid).
- Platelet parameters, however, showed significant changes over time, particularly in relation to MPC (Mean Platelet Component) in equine and canine samples, and MPV (Mean Platelet Volume) in stored samples.
- The study also found significant differences in initial platelet numbers and parameters when comparing citrate-anticoagulated blood with EDTA-anticoagulated blood.
Using CHr to detect iron deficiency
- The second part of the study established reference intervals for CHr by using samples from 53 non-anaemic dogs and 150 non-anaemic cats.
- Through the use of ROC (Receiver Operating Characteristic) curves, the optimal cut-off point was determined to be 1.22 fmol in dogs and 0.88 fmol in cats, generating high levels of sensitivity and specificity in detecting iron deficiency anemia in these animals.
Conclusion
- This study highlights the changes in various haematological parameters due to storage, indicating that certain measurements are more reliable than others.
- It also evidences the potential use of CHr as a reliable indicator for diagnosing iron deficiency anaemia in dogs and cats.
Cite This Article
APA
Prins M, van Leeuwen MW, Teske E.
(2009).
Stability and reproducibility of ADVIA 120-measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin content (CH(R)) in the diagnosis of iron deficiency.
Tijdschr Diergeneeskd, 134(7), 272-278.
Publication
Researcher Affiliations
- Department of Clinical Sciences of Companion Animals, Veterinary Faculty, Utrecht University, P.O. Box 80.154, 3508 TD Utrecht, the Netherlands.
MeSH Terms
- Animals
- Blood Cell Count / methods
- Blood Cell Count / standards
- Blood Cell Count / veterinary
- Blood Chemical Analysis / methods
- Blood Chemical Analysis / standards
- Blood Chemical Analysis / veterinary
- Blood Specimen Collection / methods
- Blood Specimen Collection / standards
- Blood Specimen Collection / veterinary
- Cat Diseases / blood
- Cat Diseases / diagnosis
- Cats
- Dog Diseases / blood
- Dog Diseases / diagnosis
- Dogs
- Erythrocyte Indices / veterinary
- Hematologic Tests / methods
- Hematologic Tests / standards
- Hematologic Tests / veterinary
- Hematology / instrumentation
- Hemoglobins / analysis
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses
- Iron / blood
- Iron Deficiencies
- ROC Curve
- Reticulocytes / chemistry
- Sensitivity and Specificity
- Species Specificity
- Temperature
- Time Factors
Citations
This article has been cited 11 times.- Pijnacker T, Bartels R, van Leeuwen M, Teske E. Identification of parameters and formulation of a statistical and machine learning model to identify Babesia canis infections in dogs using available ADVIA hematology analyzer data. Parasit Vectors 2022 Jan 29;15(1):41.
- Betting A, Schweighauser A, Francey T. Diagnostic value of reticulocyte indices for the assessment of the iron status of cats with chronic kidney disease. J Vet Intern Med 2022 Mar;36(2):619-628.
- Grebert M, Granat F, Braun JP, Leroy Q, Bourgès-Abella N, Trumel C. Validation of the Sysmex XN-V hematology analyzer for canine specimens. Vet Clin Pathol 2021 Jun;50(2):184-197.
- Strous E, Vanhoudt A, Smolenaars A, van Schaik G, Schouten M, de Pater H, Roelofs B, Nielen M. Observational Study on Variation of Longitudinal Platelet Counts in Calves over the First 14 Days of Life and Reference Intervals from Cross-Sectional Platelet and Leukocyte Counts in Dairy Calves up to Two Months of Age. Animals (Basel) 2021 Jan 29;11(2).
- Ehrmann C, Engel J, Moritz A, Roscher K. Assessment of platelet biology in equine patients with systemic inflammatory response syndrome. J Vet Diagn Invest 2021 Mar;33(2):300-307.
- Hunt A, Jugan MC. Anemia, iron deficiency, and cobalamin deficiency in cats with chronic gastrointestinal disease. J Vet Intern Med 2021 Jan;35(1):172-178.
- Athanasiou LV, Polizopoulou Z, Kalafati MR, Ntararas G, Kontos V. Effects of pre-analytical handling on selected canine hematological parameters evaluated by automatic analyzer. Vet Res Forum 2016 Fall;7(4):281-285.
- Ahmadi-Hamedani M, Mirmohammadkhani M, Kafshdouzan K, Amoozadeh P, Heydarkhani Y. Iron-Limited Erythropoiesis in Dogs and Cats: A Systematic Review and Meta-Analysis of Current Evidence Examining Mean Corpuscular Volume, Mean Reticulocyte Volume, and Reticulocyte Hemoglobin Content. J Vet Intern Med 2025 Sep-Oct;39(5):e70239.
- Jugan MC, Langston C, Plattner BL, Ford AK. Effect of Iron Deficiency on Short-Term Response to Treatment in Cats With Chronic Enteropathies. J Vet Intern Med 2025 May-Jun;39(3):e70131.
- Jensen AL, Vestergaard JD, Nielsen LN, Krogh AK, Langhorn R. In vitro-induced Heinz bodies showed no impact on feline reticulocyte haemoglobin content measurement using the Advia 2120i analyser. J Feline Med Surg 2025 Apr;27(4):1098612X251314709.
- Winzelberg Olson S, Hohenhaus AE. Feline non-regenerative anemia: Diagnostic and treatment recommendations. J Feline Med Surg 2019 Jul;21(7):615-631.
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