Staining abnormalities of dermal collagen in eosinophil- or neutrophil-rich inflammatory dermatoses of horses and cats as demonstrated with Masson’s trichrome stain.
Abstract: A retrospective study was performed on skin biopsy specimens from horses and cats having eosinophilic granulomas with 'collagen degeneration', eosinophilic skin diseases without 'collagen degeneration' and pyogranulomas without 'collagen degeneration'. Neither the appearance of collagen fibres nor the dominant polymorphonuclear cells, as seen in H & E-stained sections, were predictive of Masson's trichrome findings. Hence, a Masson's trichrome staining abnormality of collagen fibres was no more likely to be present in lesions with 'collagen degeneration' than in those lesions without 'collagen degeneration'. In addition, evaluation of trichrome-stained specimens suggested that there is no collagen degeneration in equine and feline eosinophilic granulomas and that the areas previously referred to as collagen degeneration are more appropriately called flame figures.
Blackwell Science Ltd.
Publication Date: 2000-03-01 PubMed ID: 34644847DOI: 10.1046/j.1365-3164.2000.00172.xGoogle Scholar: Lookup
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Summary
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The researchers conducted a retrospective study on skin samples from horses and cats with specific skin diseases, showing that Masson’s trichrome stain does not always correlate with collagen fiber degradation or the presence of certain white blood cells.
Research Context
- The study looked into skin conditions in horses and cats characterized by the excessive accumulation of eosinophils (‘eosinophilic granulomas‘) and inflammatory conditions (‘pyogranulomas’). These conditions often involve ‘collagen degeneration’, a deteriorating change in the structural protein in skin.
- Some conditions did not depict ‘collagen degeneration’, and these were also part of the study.
Method and Findings
- The researchers used a method called Masson’s trichrome staining to study the appearance of collagen fibers in the animal skin samples. This dye helps to differentiate between collagen and other types of tissue.
- They found that the appearance of collagen fibers or the presence of specific types of white blood cells (polymorphonuclear cells) in the H & E-stained sections was not predictive of the findings of Masson’s trichrome staining. This means that even if there appeared to be collagen degeneration or increased numbers of these white blood cells, the Masson’s trichrome stain did not always confirm these findings.
Conclusion and Significance
- The conclusion of the study challenged previous understandings. The researchers suggested that the term ‘collagen degeneration’ may not be entirely accurate when referring to changes seen in these skin conditions. Instead, the affected areas are more appropriately referred to as ‘flame figures’.
- This work is significant as it raises questions about our understanding of collagen degeneration in equine and feline eosinophilic granulomas, which could potentially lead to a rethinking of the terminology and description of these conditions in veterinary pathology.
Cite This Article
APA
Fernandez CJ, Scott DW, Erb HN.
(2000).
Staining abnormalities of dermal collagen in eosinophil- or neutrophil-rich inflammatory dermatoses of horses and cats as demonstrated with Masson’s trichrome stain.
Vet Dermatol, 11(1), 43-48.
https://doi.org/10.1046/j.1365-3164.2000.00172.x Publication
Researcher Affiliations
- Departments of Pathology.
- Clinical Sciences, College of Veterinary Medicine Cornell University, Ithaca, NY 14853, USA.
- Clinical Sciences, College of Veterinary Medicine Cornell University, Ithaca, NY 14853, USA.
References
This article includes 23 references
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