Stimulation by iodide of H(2)O(2) generation in thyroid slices from several species.
Abstract: The regulation of thyroid metabolism by iodide involves numerous inhibitory effects. However, in unstimulated dog thyroid slices, a small inconstant stimulatory effect of iodide on H(2)O(2) generation is observed. The only other stimulatory effect reported with iodide is on [1-(14)C]glucose oxidation, i.e., on the pentose phosphate pathway. Because we have recently demonstrated that the pentose phosphate pathway is controlled by H(2)O(2) generation, we study here the effect of iodide on basal H(2)O(2) generation in thyroid slices from several species. Our data show that in sheep, pig, bovine, and to a lesser extent dog thyroid, iodide had a stimulatory effect on H(2)O(2) generation. In horse and human thyroid, an inconstant effect was observed. We demonstrate in dogs that the stimulatory effect of iodide is greater in thyroids deprived of iodide, raising the possibility that differences in thyroid iodide pool may account, at least in part, for the differences between the different species studied. This represents the first demonstration of an activation by iodide of a specialized thyroid function. In comparison with conditions in which an inhibitory effect of iodide on H(2)O(2) generation is observed, the stimulating effect was observed for lower concentrations and for a shorter incubation time with iodide. Such a dual control of H(2)O(2) generation by iodide has the physiological interest of promoting an efficient oxidation of iodide when the substrate is provided to a deficient gland and of avoiding excessive oxidation of iodide and thus synthesis of thyroid hormones when it is in excess. The activation of H(2)O(2) generation may also explain the well described toxic effect of acute administration of iodide on iodine-depleted thyroids.
Publication Date: 2000-04-06 PubMed ID: 10751204DOI: 10.1152/ajpendo.2000.278.4.E692Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates the role of iodide in stimulating the production of H(2)O(2), a crucial chemical process in the thyroid glands of animals and humans. The findings suggest that iodide has a two-fold effect; promoting efficient oxidation in deficient thyroids and preventing excessive hormone production in iodine-rich thyroids.
Objective of the Study
- The study aimed to understand the effect of iodide on the stimulation of hydrogen peroxide (H(2)O(2)) in thyroid slices from various species including sheep, pig, bovine and dog. The function of hydrogen peroxide is crucial because it aids the regulation of thyroid metabolism by the process of iodide oxidation.
Key Findings
- The outcome exhibited a stimulatory effect of iodide on H(2)O(2) generation in the thyroid glands of the specified species. It also demonstrated greater stimulatory effects in iodide-deficient thyroids, particularly in dogs, implying that the thyroid iodide pool could influence the observed differences across the species.
- In contrast, with horse and human thyroids, an inconsistent effect was observed. The difference in the results could be due to variations in thyroid iodide pools among different species.
Dual Control of Iodide
- The study uncovered the dual nature of iodide control of H(2)O(2) generation in thyroid glands. Lower concentrations of iodide and short incubation time stimulated the generation, whereas inhibitory effect was observed at higher concentrations and longer incubation time with iodide.
- This dual control mechanism has potential physiological benefits, enabling an efficient oxidation of iodide when the substrate is provided to a deficient gland and preventing over-oxidization and excessive synthesis of thyroid hormones when iodide is in excess.
Potential Implications and Conclusions
- This research provides the first evidence of iodide activation contributing to a specialized thyroid function. The activation of H(2)O(2) generation may explain the toxic effect of acute administration of iodide on iodine-depleted thyroids.
Cite This Article
APA
Corvilain B, Collyn L, van Sande J, Dumont JE.
(2000).
Stimulation by iodide of H(2)O(2) generation in thyroid slices from several species.
Am J Physiol Endocrinol Metab, 278(4), E692-E699.
https://doi.org/10.1152/ajpendo.2000.278.4.E692 Publication
Researcher Affiliations
- Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, School of Medicine, Erasme University Hospital, Free University of Brussels, 1070 Brussels, Belgium. bcorvila@ulb.ac.be
MeSH Terms
- Animals
- Carbachol / pharmacology
- Cattle
- Cyclic AMP / metabolism
- Dogs
- Horses
- Humans
- Hydrogen Peroxide / metabolism
- In Vitro Techniques
- Inositol Phosphates / metabolism
- Iodides / pharmacology
- Muscarinic Agonists / pharmacology
- Oxidation-Reduction
- Phosphatidylinositol 4,5-Diphosphate / metabolism
- Sheep
- Species Specificity
- Thyroid Gland / drug effects
- Thyroid Gland / metabolism
- Thyrotropin / pharmacology
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