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International journal of systematic and evolutionary microbiology2009; 59(Pt 11); 2899-2903; doi: 10.1099/ijs.0.008862-0

Streptomyces atriruber sp. nov. and Streptomyces silaceus sp. nov., two novel species of equine origin.

Abstract: Two actinomycete strains, NRRL B-24165(T) and NRRL B-24166(T), isolated from lesions on equine placentas in Kentucky, USA, were analysed using a polyphasic taxonomic approach. On the basis of phylogenetic analysis of 16S rRNA gene sequences, morphological observations and the presence of ll-diaminopimelic acid as the diagnostic diamino acid in whole-cell hydrolysates, the new isolates clearly belonged to the genus Streptomyces. Analyses of the phylogenetic positions of strains NRRL B-24165(T) and NRRL B-24166(T) based on 16S rRNA gene sequences of all recognized species of the genus Streptomyces, as well as evaluation of morphological and physiological characteristics, demonstrated that the new isolates could be differentiated from all recognized species and therefore represented novel species. It is proposed that the new strains represent two novel species for which the names Streptomyces atriruber sp. nov. (type strain NRRL B-24165(T)=DSM 41860(T)=LDDC 6330-99(T)) and Streptomyces silaceus sp. nov. (NRRL B-24166(T)=DSM 41861(T)=LDDC 6638-99(T)) are proposed. The species names are based on the distinctive colours of the substrate mycelium of these strains, dark red and deep orange-yellow, respectively.
Publication Date: 2009-07-23 PubMed ID: 19628594DOI: 10.1099/ijs.0.008862-0Google Scholar: Lookup
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  • Journal Article

Summary

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The research identified and examined two new strains of the Streptomyces bacteria that were isolated from lesions on horse placentas. The strains, named Streptomyces atriruber and Streptomyces silaceus, were defined by their distinctive colors, dark red and deep orange-yellow respectively.

Research Methodology

  • The scientists used a polyphasic taxonomic approach, which considers phenotypic and genotypic information, to analyze the new actinomycete strains.
  • The phylogenetic analysis of 16S rRNA gene sequences was one of the key techniques employed. This involved comparing the genetic information of the new strains with existing genetic data of known Streptomyces species.
  • Morphological observations were also used to identify the new strains. The researchers closely examined the physical structure and properties of the strains, specifically their distinctive dark red and deep orange-yellow colors.
  • The chemical structure of the strains was investigated further by identifying ll-diaminopimelic acid – a diamino acid common in the cell walls of bacteria – in the whole-cell hydrolysates.

Key Findings

  • Phylogenetic analysis revealed the new strains were part of the Streptomyces genus, a highly diverse group of bacteria known to produce over two-thirds of the clinically useful antibiotics of natural origin.
  • The study confirmed that the strains differ from all existing known species in terms of both the genetic make-up and morphological characteristics, therefore representing novel species within the Streptomyces genus.
  • Finally, the strains were named Streptomyces atriruber and Streptomyces silaceus, originating from their distinctive dark red and deep orange-yellow colors respectively.

Significance and Implications

  • The discovery of the new species could potentially contribute to the production of new antibiotics to fight against drug-resistant diseases, considering the well-known antibiotic-producing property of Streptomyces species.
  • The unique genetic makeup and physical properties of these strains could provide valuable insights into the biology and ecology of Streptomyces bacteria, and could serve as an important resource for future research in taxonomy, genetics, and microbiology.

Cite This Article

APA
Labeda DP, Price NP, Kroppenstedt RM, Donahue JM, Williams NM, Sells SF. (2009). Streptomyces atriruber sp. nov. and Streptomyces silaceus sp. nov., two novel species of equine origin. Int J Syst Evol Microbiol, 59(Pt 11), 2899-2903. https://doi.org/10.1099/ijs.0.008862-0

Publication

ISSN: 1466-5026
NlmUniqueID: 100899600
Country: England
Language: English
Volume: 59
Issue: Pt 11
Pages: 2899-2903

Researcher Affiliations

Labeda, D P
  • National Center for Agricultural Utilization Research, USDA-Agricultural Research Service, Peoria, IL 61604, USA. David.Labeda@ars.usda.gov
Price, N P
    Kroppenstedt, R M
      Donahue, J M
        Williams, N M
          Sells, S F

            MeSH Terms

            • Animals
            • DNA, Bacterial / genetics
            • DNA, Ribosomal / genetics
            • Female
            • Horses / microbiology
            • Molecular Sequence Data
            • Phylogeny
            • Placenta / microbiology
            • Pregnancy
            • RNA, Ribosomal, 16S / genetics
            • Streptomyces / classification
            • Streptomyces / genetics
            • Streptomyces / isolation & purification

            Citations

            This article has been cited 1 times.
            1. El-Sheikh Ali H, Loux SC, Kennedy L, Scoggin KE, Dini P, Fedorka CE, Kalbfleisch TS, Esteller-Vico A, Horohov DW, Erol E, Carter CN, Smith JL, Ball BA. Transcriptomic analysis of equine chorioallantois reveals immune networks and molecular mechanisms involved in nocardioform placentitis.. Vet Res 2021 Jul 8;52(1):103.
              doi: 10.1186/s13567-021-00972-4pubmed: 34238364google scholar: lookup