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Studies on equine recurrent uveitis. I: Levels of immunoglobulin and albumin in the aqueous humor of horses with and without intraocular disease.
Abstract: A radioimmunoassay was developed for detection of immunoglobulin in the aqueous of normal horses and horses with intraocular diseases. Levels of albumin were detected by radial immunodiffusion. Results of assays on samples from normal eyes from which aqueous was obtained by paracentesis under anesthesia were 32.10 +/- 21.50 microgram/ml for IgG, 0.05 +/- 0.01 microgram/ml for IgM, 0.04 +/- 0.02 microgram/ml for IgA and 34.0 +/- 38.0 microgram/ml for albumin. Results in 138 normal eyes sampled post mortem were 41.56 +/- 38.65 microgram/ml for IgG, 0.18 +/- 0.43 microgram/ml for IgM, 0.46 +/- 1.45 microgram/ml for IgE and 184 +/- 240 microgram/ml for albumin. Levels in abnormal eyes were some 50-120% greater, and very high levels were noted in cases of clinically documented uveitis. The aqueous to serum ratio of the proteins measured generally varied in relation to the molecular weights and was increased in diseased eyes. The ratio of IgG/albumin in the diseased eyes was less which suggested leakage of protein from an impaired blood/aqueous barrier rather than intraocular antibody synthesis.
Publication Date: 1985-10-01 PubMed ID: 4064727DOI: 10.3109/02713688509003347Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study presents a method for detecting proteins in the eye fluid of horses and compares the protein levels between healthy horses and those suffering from intraocular diseases. The recorded protein levels indicate that the presence of such diseases significantly increases protein concentration, with particularly high levels observed in instances of uveitis (inflammation of the middle layer of the eye).
Methodology
- The researchers used a technique called radioimmunoassay to detect the presence and measure the amount of immunoglobulin, a type of protein used by the immune system, in the aqueous humor of horses. This is the clear fluid in the front and rear chambers of the eye.
- They also measured the levels of another protein, albumin, using a method called radial immunodiffusion.
Findings
- They conducted tests on samples from healthy horse eyes, which were obtained under anesthesia through a method called paracentesis. They found average concentrations for various immunoglobulins (IgG, IgM, and IgA) and albumin.
- These tests were also performed on eye samples obtained post-mortem. The results showed higher average concentrations compared to the live-sampled eyes, especially for IgG and albumin.
- The protein levels in the eyes affected by intraocular diseases were found to be 50-120% higher than those in healthy eyes. The extremely high protein levels were observed in the eyes of horses suffering from uveitis.
Interpretation
- The ratio of proteins measured in the aqueous humor compared to serum (a component of blood) generally increased with the proteins’ molecular weights, particularly in diseased eyes.
- The ratio of IgG to albumin was lower in diseased eyes compared to healthy ones. The researchers interpret this as an indication of protein leaking from an impaired blood/aqueous barrier, as opposed to the horse’s body synthesizing antibodies within the eye.
Implications
- This study provides a methodology to detect and measure protein levels in the eyes of horses, which could be useful for diagnosing eye diseases.
- The findings suggest that increased protein levels, particularly of certain types of immunoglobulins and albumin, could be a marker for intraocular diseases such as uveitis in horses.
Cite This Article
APA
Halliwell RE, Hines MT.
(1985).
Studies on equine recurrent uveitis. I: Levels of immunoglobulin and albumin in the aqueous humor of horses with and without intraocular disease.
Curr Eye Res, 4(10), 1023-1031.
https://doi.org/10.3109/02713688509003347 Publication
Researcher Affiliations
MeSH Terms
- Albumins / metabolism
- Animals
- Aqueous Humor / metabolism
- Disease Models, Animal
- Female
- Horse Diseases / metabolism
- Horses
- Immunoglobulins / metabolism
- Male
- Radioimmunoassay
- Recurrence
- Reference Values
- Uveitis / metabolism
- Uveitis / veterinary
Grant Funding
- 5 R01 EY04018 / NEI NIH HHS
Citations
This article has been cited 2 times.- Wollanke B, Gerhards H, Ackermann K. Infectious Uveitis in Horses and New Insights in Its Leptospiral Biofilm-Related Pathogenesis. Microorganisms 2022 Feb 7;10(2).
- Hanna PE, Bellamy JE, Donald A. Postmortem eyefluid analysis in dogs, cats and cattle as an estimate of antemortem serum chemistry profiles. Can J Vet Res 1990 Oct;54(4):487-94.
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