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Iranian journal of veterinary research2016; 17(1); 59-61; doi: 10.1155/2013/713959.

Successful management of an equine carpal chip fracture by intra-articularly injected adipose-derived stromal vascular fraction after arthroscopic removal.

Abstract: Carpal chip fractures are common causes of lameness in racehorses. Due to disadvantages in surgical management, adjuvant treatment modalities are usually necessary. Adipose-derived stem cells (ADSCs) have the potential to differentiate into other cell types including bone and cartilage cells. Adipose-derived stromal vascular fraction (SVF) is produced during ADSCs isolation from adipose tissue. The purpose of this report was to present the successful management of a grade III chip fracture in the right carpus of a 5-year-old Thoroughbred gelding by intra-articularly injected autologous SVF one month after the arthroscopic removal of the fracture. This treatment resulted in lameness improvement and short rehabilitation period to previous racing activities. High performance levels and no recurrent injuries were recorded during a twenty month follow-up period.
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Publication Date: 2016-09-23 PubMed ID: 27656232PubMed Central: PMC4898023DOI: 10.1155/2013/713959.Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study discusses the successful treatment of a severe carpal chip fracture in a racehorse using adipose-derived stromal vascular fraction (SVF), a byproduct of stem cells, which was injected into the affected area after surgical removal of the fractured chip. The treatment resulted in improved lameness and enabled the horse to quickly return to racing with no recurrent injuries over a twenty-month period.

Background

  • In racehorses, carpal chip fractures are a common cause of lameness. These injuries can often cause long lay-off periods and negatively affect the performance of the horse.
  • Traditional surgical treatments have disadvantages, often necessitating supplementary treatments or therapies.
  • Stem cell therapy, particularly adipose-derived stem cells (ADSCs), has shown promise in this area. ADSCs can differentiate into various cell types, including bone and cartilage cells, making them potentially effective in treating such injuries.
  • During the stem cell isolation process from adipose (fat) tissues, the adipose-derived stromal vascular fraction (SVF) is produced, which has shown potential in treatment.

Methodology and Findings

  • In this case study, the researchers document the effective management of a grade III carpal chip fracture – a serious injury – in a five-year-old Thoroughbred racehorse.
  • The damaged chip was first removed arthroscopically (a minimally invasive surgical procedure).
  • One month after the surgery, autologous (stemming from the horse itself) SVF was injected into the joint of the treated limb.
  • The use of the horse’s own SVF cells avoided issues of immune rejection or disease transmission that could have occurred if cells from another horse were used.

Results and Conclusion

  • The treatment resulted in a considerable improvement in the horse’s lameness, allowing it to return to racing much quicker than it might have following traditional surgical treatments alone.
  • The researchers observed high performance levels in the horse following treatment and rehabilitation, indicating an effective recovery.
  • No recurrent injuries were recorded during the twenty-month follow-up period, demonstrating the treatment’s long-term effectiveness.
  • This case study shows the promising potential of using SVF therapy as an adjuvant treatment for healing carpal chip fractures in racehorses.

Cite This Article

APA
Tyrnenopoulou P, Karayannopoulou M, Angelopoulou S, Pyrros A, Mparous E, Koliakos G, Diakakis N. (2016). Successful management of an equine carpal chip fracture by intra-articularly injected adipose-derived stromal vascular fraction after arthroscopic removal. Iran J Vet Res, 17(1), 59-61. https://doi.org/10.1155/2013/713959.

Publication

Iranian journal of veterinary research
ISSN: 1728-1997
NlmUniqueID: 101660030
Country: Iran
Language: English
Volume: 17
Issue: 1
Pages: 59-61

Researcher Affiliations

Tyrnenopoulou, P
  • Post-Graduate Student, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece;
Karayannopoulou, M
  • Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece;
Angelopoulou, S
  • Veterinary Clinic, Athens Horse Racing Track, Markopoulo Mesogaias 190 03, Athens, Greece;
Pyrros, A
  • Veterinary Clinic, Athens Horse Racing Track, Markopoulo Mesogaias 190 03, Athens, Greece;
Mparous, E
  • Veterinary Clinic, Athens Horse Racing Track, Markopoulo Mesogaias 190 03, Athens, Greece;
Koliakos, G
  • Department of Biochemistry, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.
Diakakis, N
  • Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece;

References

This article includes 19 references
  1. Anonymous . Guide for veterinary service and judging of equestrian events: definition and classification of lameness. 4th Edn. Lexington, KY: American Association of Equine Practitioners (AAEP); 1991. 19 pp.
  2. Cui L, Wu Y, Cen L, Zhou H, Yin S, Liu G, Liu W, Cao Y. Repair of articular cartilage defect in non-weight bearing areas using adipose derived stem cells loaded polyglycolic acid mesh. Biomaterials 2009;30:2683–2693.
    pubmed: 19217157
  3. Dragoo JL, Carlson G, McCormick F, Khan-Farooqi H, Zhu M, Zuk PA, Benhaim P. Healing full-thickness cartilage defects using adipose-derived stem cells. Tissue Eng 2007;13:1615–1621.
    pubmed: 17518742
  4. Erickson GR, Gimble JM, Franklin DM, Rice HE, Awad H, Guilak F. Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem. Biophys. Res. Commun. 2002;290:763–769.
    pubmed: 11785965
  5. Frisbie DD, Kisiday JD, Kawcak CE, Werpy NM, McIlwraith CW. Evaluation of adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis. J. Orthop. Res. 2009;27:1675–1680.
    pubmed: 19544397
  6. Gimble JM, Bunnell BA, Chiu ES, Guilak F. Adipose-derived stromal vascular fraction cells and stem cells: let’s not get lost in translation. Stem Cells 2011;29:749–754.
    pubmed: 21433220
  7. Jurgens WJ, van Dijk A, Doulabi BZ, Niessen FB, Ritt MJ, van Milligen FJ, Helder MN. Freshly isolated stromal cells from the infrapatellar fat pad are suitable for a one-step surgical procedure to regenerate cartilage tissue. Cytotherapy 2009;11:1052–1064.
    pubmed: 19929469doi: 10.3109/14653240903219122google scholar: lookup
  8. Kol A, Walker NJ, Galuppo LD, Clark KC, Buerchler S, Bernanke A, Borjesson DL. Autologous point-of-care cellular therapies variably induce equine mesenchymal stem cell migration, proliferation and cytokine expression. Equine Vet. J. 2012.
    pubmed: 22780195doi: 10.1111/j.2042-3306.2012.00600.xgoogle scholar: lookup
  9. McIlwraith CW. Diagnostic and surgical arthroscopy of the carpal joint. 2005. In: McIlWraith, CW, Nixon, AJ, Wright, IM, editors. Diagnostic and surgical arthroscopy in the horse. 3rd Edn. Edinburgh: Mosby Elsevier; pp. 347–365.
  10. McIlWraith CW, Bramlage LR. Surgical treatment of joint injury. 1996. In: McIlWraith, CW, Trotter, GW, editors. Joint disease in the horse. 1st Edn. Philadelphia: Saunders; pp. 292–316.
  11. McIlwraith CW, Foerner JJ, Davis M. Osteochondritis dissecans of the tarsocrural joint: results of treatment with arthroscopic surgery. Equine Vet. J. 1991;23:155–162.
    pubmed: 1884694
  12. McIlwraith CW, Yovich JV, Martin GS. Arthroscopic surgery for the treatment of osteochondral chip fractures in the equine carpus. J. Am. Vet. Med. Assoc. 1987;191:531–540.
    pubmed: 3667408
  13. Mehlhorn AT, Zwingmann J, Finkenzeller G, Niemeyer P, Danuer M, Stark B, Südkamp NP, Schmal H. Chondrogenesis of adipose-derived adult stem cells in a poly-lactide-co-glycolide scaffold. Tissue Eng.-Part A 2009;191:1159–1167.
    pubmed: 19132918
  14. Monteiro SO, Bettencourt EV, Lepage OM. Biologic strategies for intra-articular treatment and cartilage repair. J. Equine Vet. Sci. 2015;35:175–190.
  15. Sofa N, Kuttapitiya A. Future directions for the management of pain in osteoarthritis. Int. J. Clin. Rheumtol. 2014;9:197–276.
    pmc: PMC4089899pubmed: 25018771
  16. Tzouvelekis A, Koliakos G, Ntolios P, Baira E, Bouros E, Oikonomou A, Zissimopoulos A, Kolios G, Kakagia D, Paspaliaris V, Kotsianidis I, Froudarakis M, Bouros D. Stem cell therapy for idiopathic pulmonary fibrosis: a protocol proposal. J. Transl. Med. 2011;9:182–190.
    pmc: PMC3213183pubmed: 22017817
  17. Veronesi F, Maglio M, Tschon M, Aldini NN, Fini M. Adipose-derived mesenchymal stem cells for cartilage tissue engineering: state-of-the-art in in vivo studies (Review). J. Biomed. Mater. Res. A 2014;102:2448–2466.
    pubmed: 23894033
  18. Zheng B, Cao B, Li G, Huard J. Mouse adipose-derived stem cells undergo multilineage differentiation in vitro but primarily osteogenic and chond-rogenic differentiation in vivo. Tissue Eng. 2006;12:1891–1901.
    pubmed: 16889519
  19. Zuk P. Adipose-derived stem cells in tissue regeneration: a review. ISRN Stem Cells 2013;Article ID 713959, 35 pages.
    doi: 10.1155/2013/713959.google scholar: lookup

Citations

This article has been cited 3 times.
  1. Veronesi F, Maglio M, Contartese D, Martini L, Muttini A, Fini M. Stromal Vascular Fraction and Amniotic Epithelial Cells: Preclinical and Clinical Relevance in Musculoskeletal Regenerative Medicine. Stem Cells Int 2021;2021:6632052.
    doi: 10.1155/2021/6632052pubmed: 33688354google scholar: lookup
  2. Kamenaga T, Kuroda Y, Nagai K, Tsubosaka M, Takashima Y, Kikuchi K, Fujita M, Ikuta K, Anjiki K, Maeda T, Nakano N, Takayama K, Hashimoto S, Hayashi S, Matsushita T, Niikura T, Kuroda R, Matsumoto T. Cryopreserved human adipose-derived stromal vascular fraction maintains fracture healing potential via angiogenesis and osteogenesis in an immunodeficient rat model. Stem Cell Res Ther 2021 Feb 4;12(1):110.
    doi: 10.1186/s13287-021-02182-3pubmed: 33541427google scholar: lookup
  3. Born S, Dörfel MJ, Hartjen P, Haschemi Yekani SA, Luecke J, Meutsch JK, Westphal JK, Birkelbach M, Köhnke R, Smeets R, Krueger M. A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product. Bioimpacts 2019;9(3):161-172.
    doi: 10.15171/bi.2019.20pubmed: 31508331google scholar: lookup
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