Superovulation of cattle with equine pituitary extract and porcine ESH.
Abstract: Superovulation has been practiced in cattle for more than 50 years but the results have been highly variable. Scientists at six locations compared a horse pituitary extract (HAP) with a single batch of porcine FSH (pFSH) to determine the efficacy of these hormones to induce superovulation and to test for variability in the superovulatory response. Acetone-dried equine pituitaries were suspended in 40% ethanol containing 6% ammonium acetate, and the supernatant was mixed with 2.5 volumes of cold ethanol. The resulting precipitate was washed with cold ether and dried. Total doses of 18 mg of HAP and 36 mg of pFSH were injected intramuscularly (i.m.) over 4 days, two injections per day, and prostaglandin (PGF(2)alpha; 25 mg, i.m.) was administered on Day 3. Injections were begun on Days 6 to 13 of the estrous cycle. The overall ovulation rates (mean +/- SEM) for HAP and FSH were 8.8 +/- 0.7 and 15.1 +/- 1.0, respectively (n=231; P<0.01). Location interacted (P<0.01) with the type of gonadotropin for the ovulation rate. When expressed as a proportion of the number of corpora lutea, the total number of embryos recovered was greater (P=0.03) for pFSH than for HAP, but there was no difference in the number of Quality 1 and 2 embryos. The results show that HAP can induce a satisfactory superovulatory response, but there was no evidence of reduced variability of response to HAP compared with pFSH.
Publication Date: 1992-05-01 PubMed ID: 16727107DOI: 10.1016/0093-691x(92)90107-3Google Scholar: Lookup
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- Journal Article
Summary
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The research investigated superovulation in cattle using equine pituitary extract and porcine FSH. It found out that while both were effective in inducing superovulation, the latter resulted in a higher number of embryos, but without any observable reduction in variability compared to equine pituitary extract.
Background of the Study
- The research was focused on superovulation in cattle, a commonly practiced procedure for over half a century.
- However, the results of traditional superovulation methods have been inconsistent, leading to the exploration of different hormonal treatments.
- In this study, a horse pituitary extract (HAP) and porcine FSH (pFSH) were compared to determine their efficacy in inducing superovulation and to test the variability of the superovulatory response.
Methodology
- To conduct the experiment, acetone-dried equine pituitaries were suspended in a mixture of ethanol and ammonium acetate, and the resulting solution was mixed with cold ethanol.
- The remaining precipitate was then washed with cold ether and dried.
- 18 mg of the HAP and 36 mg of pFSH were administered through intramuscular injections over a four-day period, with an additional prostaglandin dose administered on the third day.
- The injections began on different days of the estrous cycle, ranging from Day 6 to Day 13.
Results of the Study
- The average ovulation rates observed for HAP and FSH were 8.8 and 15.1, respectively, with these results being statistically significant (P<0.01).
- However, it was found that the type of gonadotropin used had an effect on the ovulation rate depending on the location.
- When calculated as a proportion, the total number of embryos harvested was larger for pFSH than HAP.
- Interestingly, there wasn’t any difference in the number of Quality 1 and 2 embryos between the two treatments.
Conclusion
- The study concluded that both HAP and pFSH can successfully induce superovulation in cattle.
- However, no significant reduction in response variability was observed with HAP when compared with pFSH.
Cite This Article
APA
Staigmiller RB, Bellows RA, Anderson GB, Seidel GE, Foote WD, Menino AR, Wright RW.
(1992).
Superovulation of cattle with equine pituitary extract and porcine ESH.
Theriogenology, 37(5), 1091-1099.
https://doi.org/10.1016/0093-691x(92)90107-3 Publication
Researcher Affiliations
- U. S. Department of Agriculture, Agricultural Research Service Fort Keogh Livestock and Range Research Laboratory, Miles City, MT 59301 USA.
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