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Home / Equine Research Bank / Equine Vet J / Survey on 9 years of anti-doping controls in horse races i...
Equine veterinary journal2025; doi: 10.1111/evj.14496

Survey on 9 years of anti-doping controls in horse races in Italy.

Abstract: Doping in racehorses is a threat to horse welfare and the integrity of the sport. Despite its relevance, the literature on the prevalence of anti-doping violations worldwide is limited. Objective: To analyse anti-doping violations in racehorses in Italy. Methods: Retrospective observational study. Methods: Data on horse races, anti-doping testing, and confirmed violations between 2014 and 2022 were collected and analysed. Positivity rates, most common drug classes and parent drugs, and differences between trotting and galloping horses were investigated. Results: During the considered 9-year timeframe, the national laboratory analysed a total of 104,770 samples. A total of 536 horses were confirmed to be positive (92.8% positivity rate at second analysis). The average prevalence over the years was 0.48 ± 0.15% in trotters and 0.50 ± 0.15% in gallopers. Seventy-seven parent drugs, belonging to 29 different drug classes, were detected. The five most represented drug classes were steroidal anti-inflammatories (19.0%), stimulants (16.4%), NSAIDs (15.5%), anabolic steroids (9.9%) and sedatives (9.7%). The five most frequent substances were dexamethasone (8.4%), cocaine (7.1%), testosterone (6.5%), caffeine (5.6%) and theophylline (4.1%). Conclusions: Our data derive from official analyses performed in compliance with the national regulation on anti-doping controls in racehorses; information on the concentration of the detected analytes was not available. Testing only the best-placed horses does not allow for correlating drug administration and improved performance; horses with less chance of winning might slip through the control system with negative consequences on their welfare. Conclusions: The percentage of confirmed doping violations in horse races in Italy in the 9 years (2014-2022) evaluated in this study was low (0.49 ± 0.15%). Considering the drug classes found most frequently, violations might have been more often the consequence of deliberate administration rather than accidental feed contamination.
© 2025 The Author(s). Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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Publication Date: 2025-03-13 PubMed ID: 40079490DOI: 10.1111/evj.14496Google Scholar: Lookup
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Summary

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The article describes a 9-year survey on doping controls in horse races in Italy, investigating the prevalence of anti-doping violations, the most commonly found drug classes and substances, and their impact on horse welfare and racing integrity.

Research Methodology

  • The researchers carried out a retrospective observational study using data collected between 2014 and 2022. This data included details on horse races, anti-doping testing, and confirmed violations.
  • The study explored differences between types of racehorses, including trotting and galloping horses. It also looked into the positivity rates and the frequency of various drugs used.

Findings and Results

  • Over the 9-year period, the national laboratory analyzed a total of 104,770 samples from various horse races, with 536 horses confirmed as positive in anti-doping tests. This represents a 92.8% positivity rate at the second analysis.
  • The average prevalence per year was calculated to be 0.48 ± 0.15% in trotting horses, and 0.50 ± 0.15% in galloping horses.
  • There were 77 different parent drugs belonging to 29 drug classes detected during the survey. The most represented drug classes were steroidal anti-inflammatories (19.0%), stimulants (16.4%), non-steroidal anti-inflammatory drugs (NSAIDs) 15.5%, anabolic steroids (9.9%), and sedatives (9.7%).
  • The five most frequent substances encountered were dexamethasone (8.4%), cocaine (7.1%), testosterone (6.5%), caffeine (5.6%), and theophylline (4.1%).

Conclusions and Implications

  • The data for the survey was collected from official analysis performed in line with the national regulation on anti-doping controls in racehorses. The researchers did not have access to information on the concentration of the detected analytes.
  • According to the researchers, testing only the best-placed horses may not result in a complete picture of doping violations. It might also mean that horses with lower chances of winning could potentially slip through the control system, which could negatively impact their welfare.
  • Overall, the confirmed doping violations in horse races in Italy over the 9-year period were relatively low (0.49 ± 0.15%).
  • The classes of drugs most frequently found suggest that the violations were more likely due to deliberate administration rather than accidental feed contamination.

Cite This Article

APA
Roccaro M, Rinnovati R, Stucchi L, La Rocca F, Cascio G, Peli A. (2025). Survey on 9 years of anti-doping controls in horse races in Italy. Equine Vet J. https://doi.org/10.1111/evj.14496

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English

Researcher Affiliations

Roccaro, Mariana
  • Department for Life Quality Studies, Alma Mater Studiorum University of Bologna, Rimini, Italy.
Rinnovati, Riccardo
  • Department of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Ozzano dell'Emilia, Italy.
Stucchi, Luca
  • Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
La Rocca, Federica
  • Department of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Ozzano dell'Emilia, Italy.
Cascio, Giuseppe
  • Department of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Ozzano dell'Emilia, Italy.
Peli, Angelo
  • Department for Life Quality Studies, Alma Mater Studiorum University of Bologna, Rimini, Italy.

References

This article includes 27 references
  1. Italian Ministry of Agriculture and Forestry. Ministry Decree No. 797 of 16/10/2002. Regulation for the control of prohibited substances and subsequent amendments.. 2002.
  2. ACCREDIA. Laboratori di prova. 2024.
  3. Moss MS, Haywood PE. Survey of positive results from racecourse antidoping samples received at racecourse security services' laboratories. Equine Vet J 1984;16(1):39–42.
    doi: 10.1111/j.2042-3306.1984.tb01846.xgoogle scholar: lookup
  4. Lotfollahzadeh S, Mokhber‐Dezfouli MR, Tajik P, Bokaie S, Watson DG. A survey on two years of medication regulation in horse races in Iran. Equine Vet J 2010;42(2):161–163.
    doi: 10.2746/042516409x471449google scholar: lookup
  5. Taddei L, Benoit M, Sukta A, Peterson J, Gaensslen RE, Negrusz A. Detection of various performance enhancing substances in specimens collected from race horses in Illinois: a five‐year experience. J Anal Toxicol 2011;35(7):438–443.
    doi: 10.1093/anatox/35.7.438google scholar: lookup
  6. Spanoudes K, Diakakis N. Equine doping: perceptions, adverse effects and Cyprus hippodrome case review for the years 2001‐2010. J Hellenic Vet Med Soc 2015;66(1):15–21.
    doi: 10.12681/jhvms.15578google scholar: lookup
  7. Waller P, Lomnicka I, Lucas C, Johnson S, Dirikolu L. The medication violations in racehorses at Louisiana racetracks from 2016 to 2020. Vet Med Sci 2022;8(2):553–560.
    doi: 10.1002/vms3.724google scholar: lookup
  8. Hytychová T, Dosedělová K, Jahn P. Doping control in horses in The Czech Republic in 2010‐2019. Acta Vet Brno 2023;92(1):39–46.
    doi: 10.2754/avb202392010039google scholar: lookup
  9. Ministry of Agriculture, Food Sovereignty and Forestry (MASAF). List of horses positive for doping. .
  10. Toutain PL. Veterinary medicines and competition animals: the question of medication versus doping control. 2010;p. 315–339.
    doi: 10.1007/978-3-642-10324-7_13google scholar: lookup
  11. Hibbert DB, Armstrong N, Vine JH. Total CO2 measurements in horses: where to draw the line. Accred Qual Assur 2011;16(7):339–345.
    doi: 10.1007/s00769-011-0790-1google scholar: lookup
  12. Denham J, Hulme A. A systematic review and meta‐analysis on sodium bicarbonate administration and equine running performance: is it time to stop horsing around with baking soda?. J Equine Vet Sci 2020;95:103281.
    doi: 10.1016/j.jevs.2020.103281google scholar: lookup
  13. Waller CC, McLeod MD. A review of designer anabolic steroids in equine sports. Drug Test Anal 2017;9(9):1304–1319.
    doi: 10.1002/dta.2112google scholar: lookup
  14. Van der Kolk JH. Endocrine function during exercise and response to training. 2014;p. 769–785.
  15. Dirikolu L, Lehner AF. A review of current chemistry, pharmacology, and regulation of endogenous anabolic steroids testosterone, boldenone, and nandrolone in horses. J Vet Pharmacol Ther 2023;46(4):201–217.
    doi: 10.1111/jvp.13397google scholar: lookup
  16. Quinn DI, Wodak A, Day RO. Pharmacokinetic and pharmacodynamic principles of illicit drug use and treatment of illicit drug users. Clin Pharmacokinet 1997;33(5):344–400.
    doi: 10.2165/00003088-199733050-00003google scholar: lookup
  17. McKeever KH, Hinchcliff KW, Gerken DF, Sams RA. Effects of cocaine on incremental treadmill exercise in horses. J Appl Physiol 1993;75(6):2727–2733.
    doi: 10.1152/jappl.1993.75.6.2727google scholar: lookup
  18. Queiroz‐Neto A, Zamur G, Lacerda‐Neto JC, Tobin T. Determination of the highest no‐effect dose (HNED) and of the elimination pattern for cocaine in horses. J Appl Toxicol 2002;22(2):117–121.
    doi: 10.1002/jat.836google scholar: lookup
  19. Romano G, Barbera N, Lombardo I. Hair testing for drugs of abuse: evaluation of external cocaine contamination and risk of false positives. Forensic Sci Int 2001;123(2):119–129.
    doi: 10.1016/s0379-0738(01)00539-4google scholar: lookup
  20. Kollias‐Baker C, Maxwell L, Stanley S, Boone T. Detection and quantification of cocaine metabolites in urine samples from horses administered cocaine. J Vet Pharmacol Ther 2003;26(6):429–434.
    doi: 10.1046/j.0140-7783.2003.00535.xgoogle scholar: lookup
  21. Machnik M, Kaiser S, Koppe S, Kietzmann M, Schenk I, Düe M. Control of methylxanthines in the competition horse: pharmacokinetic/pharmacodynamic studies on caffeine, theobromine and theophylline for the assessment of irrelevant concentrations. Drug Test Anal 2017;9(9):1372–1384.
    doi: 10.1002/dta.2097google scholar: lookup
  22. Hertzsch R, Richter A. Systematic analysis to assess the scientific validity of the international residue limits for caffeine and theophylline in horse‐racing. Vet Rec 2019;185(8):230.
    doi: 10.1136/vr.105404google scholar: lookup
  23. McKeever KH, Malinowski K, Fenger CK, Duer WC, Maylin GA. Evaluation of cobalt as a performance enhancing drug (PED) in racehorses. Comp Exerc Physiol 2020;16(4):243–252.
    doi: 10.3920/cep200001google scholar: lookup
  24. Dirikolu L, Woods WE, Boyles J, Lehner AF, Harkins JD, Fisher M. Nonsteroidal anti‐inflammatory agents and musculoskeletal injuries in Thoroughbred racehorses in Kentucky. J Vet Pharmacol Ther 2009;32(3):271–279.
    doi: 10.1111/j.1365-2885.2008.01038.xgoogle scholar: lookup
  25. Salomonsson ML, Bondesson U, Hedeland M. Quantification of dimethylsulfoxide (DMSO) in equine plasma and urine using HILIC‐MS/MS. Drug Test Anal 2017;9(6):935–941.
    doi: 10.1002/dta.2098google scholar: lookup
  26. Soma LR, Robinson MA, You Y, Boston RC, Rudy J. Pharmacokinetics, disposition, and plasma concentrations of dimethyl sulfoxide (DMSO) in the horse following topical, oral, and intravenous administration. J Vet Pharmacol Ther 2018;41(3):384–392.
    doi: 10.1111/jvp.12476google scholar: lookup
  27. Italian Ministry of Agriculture, Food Sovereignty and Forestry. Ministry Decree No. 368162 of 13/07/2023. Regulation for the control of prohibited substances in horses.. 2023.
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