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Journal of veterinary internal medicine2015; 29(6); 1683-1688; doi: 10.1111/jvim.13627

Survival Time of Cross-Match Incompatible Red Blood Cells in Adult Horses.

Abstract: There is a markedly reduced half-life of transfused RBCs when donor and recipient cats or humans are cross-match incompatible. Only 10-20% of horses have naturally occurring alloantibodies. Therefore, cross-match testing before blood transfusion is not always performed. Objective: Cross-match incompatibility predicts shortened RBC survival time as compared to that of compatible or autologous blood. Methods: Twenty healthy adult horses. Methods: Prospective trial. Blood type, anti-RBC antibody screen (before and 1 month after transfusion) and major and minor cross-match determined 10 donor-recipient pairs. Two pairs were cross-match compatible, the remainder incompatible. Donor blood (4 L) was collected into citrate phosphate dextrose adenine-1, labeled with NHS-biotin, and transfused into recipients. Samples were collected at 1 hour and 1, 2, 3, 5, 7, 14, 21, 28, and 35 days after transfusion, and biotinylated RBCs were detected by flow cytometry. Horses were monitored for transfusion reaction during transfusion and daily for 5 days. Results: Cross-match incompatibility was significantly associated with decreased RBC survival time (P 1+) allogenic equine RBCs was 4.7 (95% CI, 3.2-6.2) days versus 33.5 (24-43) days for compatible pairings. Cross-match incompatibility was associated with acute febrile transfusion reaction (P = .0083). At day 30, only 1 horse had developed novel anti-RBC antibodies. Conclusions: Cross-match incompatibility was predictive of febrile transfusion reaction and shortened transfused RBC survival, but did not result in production of anti-RBC antibodies at 30 days. Cross-match testing before transfusion is recommended.
Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2015-10-18 PubMed ID: 26478135PubMed Central: PMC4895677DOI: 10.1111/jvim.13627Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study discusses the decreased lifespan of transferred Red Blood Cells (RBCs) in horses when the donor and the receiving horse are cross-match incompatible. Findings suggest that cross-match incompatibility can lead to shorter RBC survival time, and even trigger acute febrile transfusion reaction, but does not result in the production of anti-RBC antibodies at 30 days. The study therefore proposes cross-match testing before blood transfusion to ensure compatibility and to avoid potentially harmful effects.

Methods

  • The study was a prospective trial conducted on twenty healthy adult horses. These horses were divided into 10 donor-recipient pairs based on blood type, anti-RBC antibody screen (before and 1 month after transfusion), and major and minor cross-match.
  • Two of these pairs were cross-match compatible, while the other eight were incompatible.
  • Blood was taken from the donor horse, and was labelled with NHS-biotin; it was then transfused into the receiving horse.
  • Post-transfusion, samples were collected at given intervals to study the presence of biotinylated RBCs using flow cytometry.
  • Throughout the process, horses were carefully monitored for any signs of transfusion reaction.

Results

  • The research found a significant association between cross-match incompatibility and decreased RBC survival time.
  • The average lifespan of transferred RBCs in the case of cross-match incompatibility was around 4.7 days, much lesser than the 33.5 days recorded for compatible pairings.
  • Instances of acute febrile transfusion reaction were noticed when there was cross-match incompatibility.
  • Interestingly, at the end of 30 days, only one horse had developed new anti-RBC antibodies.

Conclusions

  • Contrary to expectation, cross-match incompatibility didn’t lead to the development of anti-RBC antibodies at 30 days’ post-transfusion.
  • However, it did lead to shorter lifespan for the transferred RBCs, and was linked to instances of acute febrile transfusion reaction.
  • In light of these findings, the study recommends conducting a cross-match test prior to the transfusion to circumvent these potentially harmful effects.

Cite This Article

APA
Tomlinson JE, Taberner E, Boston RC, Owens SD, Nolen-Walston RD. (2015). Survival Time of Cross-Match Incompatible Red Blood Cells in Adult Horses. J Vet Intern Med, 29(6), 1683-1688. https://doi.org/10.1111/jvim.13627

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 29
Issue: 6
Pages: 1683-1688

Researcher Affiliations

Tomlinson, J E
  • Department of Clinical Studies - New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA.
  • Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY.
Taberner, E
  • Department of Clinical Studies - New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA.
Boston, R C
  • Department of Clinical Studies - New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA.
Owens, S D
  • Department of Pathology, Microbiology and Immunology, UC Davis School of Veterinary Medicine, Davis, CA.
Nolen-Walston, R D
  • Department of Clinical Studies - New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA.

MeSH Terms

  • Animals
  • Blood Group Antigens / classification
  • Blood Group Incompatibility / blood
  • Blood Group Incompatibility / veterinary
  • Blood Grouping and Crossmatching / veterinary
  • Blood Transfusion / veterinary
  • Erythrocyte Aging
  • Erythrocytes
  • Horses / blood

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Citations

This article has been cited 12 times.
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  2. Kretsch CM, Alonso FH, Buktenica M, Heller MC. Agglutination and hemolytic crossmatching to determine transfusion reaction differences between large and small breed goats. J Vet Intern Med 2023 Jul-Aug;37(4):1594-1602.
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