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Veterinary journal (London, England : 1997)2013; 198(1); 217-223; doi: 10.1016/j.tvjl.2013.07.020

Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses.

Abstract: Platelet-rich plasma (PRP) products may be useful for treatment of joint disease in horses, but may contain undesirable pro-inflammatory cytokines in addition to growth factors. This study investigated whether autologous PRP increases synovial fluid growth factor and cytokine concentrations when injected into normal equine metacarpophalangeal and metatarsophalangeal (fetlock) joints. Fetlock joints of seven healthy horses received one of four treatments: saline, resting PRP, CaCl2-activated PRP or thrombin-activated PRP. Synovial fluid was sampled prior to injection and at 6, 24, 48 and 96 h post-injection. Platelet-derived growth factor (PDGF-BB), transforming growth factor β1 (TGFβ1), interleukin (IL)-6 and tumor necrosis factor α (TNFα) concentrations in synovial fluid and PRP were measured by ELISA. Synovial fluid PDGF-BB, TGFβ1, IL-6, TNFα and IL-1 concentrations were also measured in vitro after incubation for 6h with resting PRP only. Growth factor concentrations, but not cytokine concentrations, were significantly higher in activated PRP than in resting PRP samples. After intra-articular injection with resting or thrombin-activated PRP, synovial TGFβ1 increased significantly compared to baseline levels. TNFα and IL-6 were significantly increased in synovial fluid after thrombin-activated PRP injection. In vitro, growth factor concentrations increased significantly in synovial fluid after mixing with PRP, indicating that exogenous activation of PRP for intra-articular injection may be unnecessary, whereas cytokine levels did not. In conclusion, thrombin-activated PRP induced an inflammatory cytokine response in joints, whereas resting or CaCl2-activated PRP did not. Synovial growth factor levels were low overall; the reported benefits of intra-articular PRP may not be attributable to changes in local PDGF or TGFβ1 concentrations.
Copyright © 2013 Elsevier Ltd. All rights reserved.
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Publication Date: 2013-08-28 PubMed ID: 23992870DOI: 10.1016/j.tvjl.2013.07.020Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the impact of injecting autologous platelet-rich plasma (PRP) in horses‘ joint disease treatment, examining specifically how the injection influences synovial fluid growth factor and cytokine concentrations. The researchers point out that while PRP may potentially treat joint diseases in horses, it may also contain unfavorable pro-inflammatory cytokines along with growth factors.

Research Methodology

  • The study was conducted on healthy horses and involved injecting their fetlock joints with one out of four possible treatments: saline, resting PRP, CaCl2-activated PRP, or thrombin-activated PRP. The fetlock is a joint analogous to the human ankle but with a more complex structure.
  • Prior to the injection and at intervals post-injection (6, 24, 48, and 96 hours), synovial fluid samples were procured from the subjects for analysis.
  • The key growth factors and cytokines considered in the study were Platelet-Derived Growth Factor (PDGF-BB), Transforming Growth Factor β1 (TGFβ1), Interleukin 6 (IL-6), and Tumor Necrosis Factor α (TNFα). Their concentration levels in the synovial fluid and PRP were measured using the enzyme-linked immunosorbent assay (ELISA).
  • The study also assessed in-vitro synovial fluid growth factors and cytokines, IL-1 and TNFα, after a 6-hour interaction with resting PRP.

Findings

  • One notable finding from the study was that growth factor concentrations were significantly higher in activated PRP than in resting PRP, while there was no marked difference in cytokine concentrations.
  • After injecting resting or thrombin-activated PRP into a joint, the concentration of TGFβ1 in the synovial fluid showed significant increase from baseline levels.
  • However, TNFα and IL-6 concentrations showed significant increase in the synovial fluid only following thrombin-activated PRP injection.
  • When PRP was mixed with synovial fluid in an in-vitro experiment, the growth factor levels in the latter significantly spiked, suggesting that it may be unnecessary to activate PRP exogenously before injecting it into a joint, while cytokine levels remained unchanged.

Conclusion

  • The study concluded that thrombin-activated PRP seems to induce an inflammatory cytokine reaction inside the joints, which does not occur with the use of resting or CaCl2-activated PRP.
  • However, researchers noted that the overall synovial growth factor levels remained low. This suggests that the reported advantages of intra-articular PRP may not necessarily be due to changes in local PDGF or TGFβ1 concentrations.

Cite This Article

APA
Textor JA, Willits NH, Tablin F. (2013). Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses. Vet J, 198(1), 217-223. https://doi.org/10.1016/j.tvjl.2013.07.020

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 198
Issue: 1
Pages: 217-223

Researcher Affiliations

Textor, Jamie A
  • Department of Pathology, Microbiology and Immunology, University of California, Davis, CA 95616, USA. Electronic address: jamietextor@gmail.com.
Willits, Neil H
    Tablin, Fern

      MeSH Terms

      • Animals
      • Cytokines / metabolism
      • Enzyme-Linked Immunosorbent Assay / veterinary
      • Female
      • Forelimb / drug effects
      • Forelimb / immunology
      • Forelimb / metabolism
      • Hindlimb / drug effects
      • Hindlimb / immunology
      • Hindlimb / metabolism
      • Horses / metabolism
      • Injections, Intra-Articular / veterinary
      • Intercellular Signaling Peptides and Proteins / metabolism
      • Joints / drug effects
      • Joints / immunology
      • Joints / metabolism
      • Male
      • Platelet-Rich Plasma / metabolism
      • Synovial Fluid / metabolism
      • Time Factors

      Citations

      This article has been cited 25 times.
      1. Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
        doi: 10.3390/ani15243586pubmed: 41463871google scholar: lookup
      2. Bungărdean D, Pall E, Daradics Z, Popescu M, Tripon MA, Lupșan AF, Crecan CM, Morar IA, Nicolescu A, Bora FD, Marcus I. In Vitro Effects of PRP, Ozonized PRP, Hyaluronic Acid, Paracetamol, and Polyacrylamide on Equine Synovial Fluid-Derived Mesenchymal Stem Cells. Life (Basel) 2025 Oct 4;15(10).
        doi: 10.3390/life15101558pubmed: 41157231google scholar: lookup
      3. Chaimbeul SF, Rodrigues NNP, Thurston DD, Scoggin KE, Janes J, Jacobs CA, MacLeod JN, Stone AV, Menarim BC. PPARγ Agonism Modulates Synovial Macrophage and Cartilage Responses in an Equine Model of Synovial Inflammation-Implications for Joint Therapy. Biomolecules 2025 Sep 1;15(9).
        doi: 10.3390/biom15091267pubmed: 41008574google scholar: lookup
      4. Carmona JU, López C. Platelet-Rich Plasma in Equine Osteoarthritis: A Systematic Review of Clinical and Experimental Evidence. Animals (Basel) 2025 Sep 9;15(18).
        doi: 10.3390/ani15182647pubmed: 41007891google scholar: lookup
      5. Ospina J, Carmona JU, López C. Short-Term Effects of Two COX-2 Selective Non-Steroidal Anti-Inflammatory Drugs on the Release of Growth Factors and Cytokines from Canine Platelet-Rich Gel Supernatants. Gels 2024 Jun 12;10(6).
        doi: 10.3390/gels10060396pubmed: 38920942google scholar: lookup
      6. van der Kraan PM, van Caam APM, Blaney Davidson EN, van den Bosch MHJ, van de Loo FAJ. Growth factors that drive aggrecan synthesis in healthy articular cartilage. Role for transforming growth factor-β?. Osteoarthr Cartil Open 2024 Jun;6(2):100459.
        doi: 10.1016/j.ocarto.2024.100459pubmed: 38486843google scholar: lookup
      7. Mayet A, Zablotski Y, Roth SP, Brehm W, Troillet A. Systematic review and meta-analysis of positive long-term effects after intra-articular administration of orthobiologic therapeutics in horses with naturally occurring osteoarthritis. Front Vet Sci 2023;10:1125695.
        doi: 10.3389/fvets.2023.1125695pubmed: 36908512google scholar: lookup
      8. Ma Q, Hei X, Zhu S, Tian X, Chen Y, Zhu N, Zhang J, Feng P, Liu Y, Xing L, Li X, Liu Q, Li F, Li X, Lai Z, Lin R, Xu J. The Clinical Efficacy of Platelet-Rich Plasma versus Conventional Drug Injection in the Treatment of Knee Osteoarthritis: A Study Protocol for a Randomized Controlled Trial. Evid Based Complement Alternat Med 2022;2022:8767137.
        doi: 10.1155/2022/8767137pubmed: 35656456google scholar: lookup
      9. Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front Vet Sci 2021;8:641818.
        doi: 10.3389/fvets.2021.641818pubmed: 33869321google scholar: lookup
      10. Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. Single and repeated intra-articular injections in the tarsocrural joint with allogeneic and autologous equine bone marrow-derived mesenchymal stem cells are safe, but did not reduce acute inflammation in an experimental interleukin-1β model of synovitis. Equine Vet J 2020 Jul;52(4):601-612.
        doi: 10.1111/evj.13222pubmed: 31821594google scholar: lookup
      11. Seidel SRT, Vendruscolo CP, Moreira JJ, Fülber J, Ottaiano TF, Oliva MLV, Michelacci YM, Baccarin RYA. Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma?. Vet Sci 2019 Aug 21;6(3).
        doi: 10.3390/vetsci6030068pubmed: 31438534google scholar: lookup
      12. Smit Y, Marais HJ, Thompson PN, Mahne AT, Goddard A. Clinical findings, synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis. J S Afr Vet Assoc 2019 May 23;90(0):e1-e9.
        doi: 10.4102/jsava.v90i0.1721pubmed: 31170778google scholar: lookup
      13. Niemelä TM, Tulamo RM, Carmona JU, López C. Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses. Acta Vet Scand 2019 May 30;61(1):24.
        doi: 10.1186/s13028-019-0460-6pubmed: 31146775google scholar: lookup
      14. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front Vet Sci 2018;5:70.
        doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
      15. Seabaugh KA, Thoresen M, Giguère S. Extracorporeal Shockwave Therapy Increases Growth Factor Release from Equine Platelet-Rich Plasma In Vitro. Front Vet Sci 2017;4:205.
        doi: 10.3389/fvets.2017.00205pubmed: 29270410google scholar: lookup
      16. Hauschild G, Geburek F, Gosheger G, Eveslage M, Serrano D, Streitbürger A, Johannlükens S, Menzel D, Mischke R. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations. BMC Vet Res 2017 Jan 5;13(1):7.
        doi: 10.1186/s12917-016-0920-4pubmed: 28056978google scholar: lookup
      17. Muto T, Kokubu T, Mifune Y, Inui A, Sakata R, Harada Y, Takase F, Kurosaka M. Effects of platelet-rich plasma and triamcinolone acetonide on interleukin-1ß-stimulated human rotator cuff-derived cells. Bone Joint Res 2016 Dec;5(12):602-609.
        doi: 10.1302/2046-3758.512.2000582pubmed: 27965219google scholar: lookup
      18. Mariani E, Canella V, Cattini L, Kon E, Marcacci M, Di Matteo B, Pulsatelli L, Filardo G. Leukocyte-Rich Platelet-Rich Plasma Injections Do Not Up-Modulate Intra-Articular Pro-Inflammatory Cytokines in the Osteoarthritic Knee. PLoS One 2016;11(6):e0156137.
        doi: 10.1371/journal.pone.0156137pubmed: 27258008google scholar: lookup
      19. Muto T, Kokubu T, Mifune Y, Inui A, Sakata R, Harada Y, Takase F, Ueda Y, Kuroda R, Kurosaka M. Can Platelet-Rich Plasma Protect Rat Achilles Tendons From the Deleterious Effects of Triamcinolone Acetonide?. Orthop J Sports Med 2015 Jul;3(7):2325967115590968.
        doi: 10.1177/2325967115590968pubmed: 26673355google scholar: lookup
      20. Moraes AP, Moreira JJ, Brossi PM, Machado TS, Michelacci YM, Baccarin RY. Short- and long-term effects of platelet-rich plasma upon healthy equine joints: Clinical and laboratory aspects. Can Vet J 2015 Aug;56(8):831-8.
        pubmed: 26246629
      21. Ríos DL, López C, Álvarez ME, Samudio IJ, Carmona JU. Effects over time of two platelet gel supernatants on growth factor, cytokine and hyaluronan concentrations in normal synovial membrane explants challenged with lipopolysaccharide. BMC Musculoskelet Disord 2015 Jun 20;16:153.
        doi: 10.1186/s12891-015-0605-3pubmed: 26092588google scholar: lookup
      22. Ríos DL, López C, Carmona JU. Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants. Vet Med Int 2015;2015:547052.
        doi: 10.1155/2015/547052pubmed: 26090267google scholar: lookup
      23. Marmotti A, Rossi R, Castoldi F, Roveda E, Michielon G, Peretti GM. PRP and articular cartilage: a clinical update. Biomed Res Int 2015;2015:542502.
        doi: 10.1155/2015/542502pubmed: 26075244google scholar: lookup
      24. Brossi PM, Moreira JJ, Machado TS, Baccarin RY. Platelet-rich plasma in orthopedic therapy: a comparative systematic review of clinical and experimental data in equine and human musculoskeletal lesions. BMC Vet Res 2015 Apr 22;11:98.
        doi: 10.1186/s12917-015-0403-zpubmed: 25896610google scholar: lookup
      25. O'Shaughnessey K, Matuska A, Hoeppner J, Farr J, Klaassen M, Kaeding C, Lattermann C, King W, Woodell-May J. Autologous protein solution prepared from the blood of osteoarthritic patients contains an enhanced profile of anti-inflammatory cytokines and anabolic growth factors. J Orthop Res 2014 Oct;32(10):1349-55.
        doi: 10.1002/jor.22671pubmed: 24981198google scholar: lookup
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