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Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2000; 8(1); 45-55; doi: 10.1023/a:1009275102977

Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes.

Abstract: The hypothesis that the conservation of sex-chromosome-linked genes among placental mammals could be extended to the horse genome was tested using the UCDavis horse-mouse somatic cell hybrid (SCH) panel. By exploiting the fluorescence in-situ hybridization (FISH) technique to localize an anchor locus, X-inactivation-specific transcript (XIST) on the horse X chromosome, together with the fragmentation and translocation of the X- and Y-chromosome fragments in a somatic cell hybrid panel, we regionally assigned 13 type I and 13 type II (microsatellite) markers to the horse X- and Y-chromosomes. The synteny groups that correspond to horse X- and Y-chromosomes were identified by synteny mapping of sex-specific loci zinc finger protein X-linked (ZFX), zinc finger protein Y-linked (ZFY) and sex-determining region Y (SRY) on the SCH panel. A non-pseudoautosomal gene in the human steroid sulfatase (STS) was identified in both X- and Y-chromosome-containing clones. The regional order of the X-linked type I markers examined in this study, from Xp- to Xq-distal, was [STS-X, the voltage-gated chloride channel 4 (CLCN4)], [ZFX, delta-aminolevulinate synthase 2 (ALAS2)], XIST, coagulation factor IX (F9) and [biglycan (BGN), equine F18, glucose-6-phosphate dehydrogenase (G6PD)] (precise marker order could not be determined for genes within the same brackets). The order of the Y-linked type I markers was STS-Y, SRY and ZFY These orders are the same arrangements as reported for the human X- and Y-chromosomes, supporting the conservation of genomic organization between the human and the horse sex chromosomes. Regional ordering of X-linked type I and microsatellite markers provides the first integration of type I and type II markers in the horse X chromosome.
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Publication Date: 2000-03-24 PubMed ID: 10730588DOI: 10.1023/a:1009275102977Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates whether the conservation of sex-chromosome-linked genes found in other placental mammals also extends to horses. Using a combination of scientific techniques, it identifies and places markers on horse X- and Y-chromosomes, confirming that the genetic organization of horse sex chromosomes is similar to that of humans.

Techniques Utilized

  • The researchers employed a somatic cell hybrid (SCH) panel, specifically developed at UCDavis, to study hybrid cells containing both horse and mouse genetic material.
  • As part of the study, fluorescence in-situ hybridization (FISH) was used. This is a modern technique that involves painting chromosomes with fluorescent markers, allowing researchers to visualize specific genetic sequences.
  • Using FISH, the researchers were able to localize an anchor locus – in this case, the X-inactivation-specific transcript (XIST) gene on the horse X chromosome.

Identification and Ordering of Genetic Markers

  • Using the SCH panel, the team was successful in assigning 13 type I and 13 type II (microsatellite) markers to the horse X- and Y-chromosomes.
  • The markers were identified by mapping of sex-specific loci, including the zinc finger protein X-linked (ZFX), zinc finger protein Y-linked (ZFY) and sex-determining region Y (SRY), onto the SCH panel.
  • They were also able to identify a gene commonly found on the X and Y chromosomes in humans – the steroid sulfatase (STS).
  • The team was able to broadly order the markers from Xp- to Xq-distal along the X chromosome and identify the order of the Y-linked type I markers.
  • Interestingly, the order in which these markers were found is consistent with what is observed on human sex chromosomes, suggesting a conservation of genomic organization between humans and horses.

Significance of Findings

  • This research represents the first integration of type I and type II markers on the horse X chromosome, advancing our understanding of horse genetic makeup.
  • The conservation of genomic organization between human and horse sex chromosomes could have a range of implications, particularly for comparative genomics and potentially for veterinary medicine.

Cite This Article

APA
Shiue Y-L , Millon LV, Skow LC, Honeycutt D, Murray JD, Bowling AT. (2000). Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes. Chromosome Res, 8(1), 45-55. https://doi.org/10.1023/a:1009275102977

Publication

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 0967-3849
NlmUniqueID: 9313452
Country: Netherlands
Language: English
Volume: 8
Issue: 1
Pages: 45-55

Researcher Affiliations

Shiue Y-L,
  • Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California, Davis 95616-8744, USA.
Millon, L V
    Skow, L C
      Honeycutt, D
        Murray, J D
          Bowling, A T

            MeSH Terms

            • Animals
            • Chromosome Mapping
            • Genetic Markers
            • Horses / genetics
            • Humans
            • Hybrid Cells
            • In Situ Hybridization, Fluorescence
            • Microsatellite Repeats
            • Pseudogenes
            • X Chromosome
            • Y Chromosome

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            Citations

            This article has been cited 4 times.
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              doi: 10.1073/pnas.0308513100pubmed: 14983019google scholar: lookup
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