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Iranian journal of parasitology2023; 18(1); 66-75; doi: 10.18502/ijpa.v18i1.12381

Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses.

Abstract: This study aimed to develop new complex preparations of ivermectin, niclosamide and albendazole based on solid-phase mechanochemical technology, and to evaluate their efficacy against equine nematodosis and cestodosis. Unassigned: Novel formulation of antiparasitic paste were prepared using joint mechano-chemical treatment of ivermectin (0.2 mg/kg bodyweight; BW), niclosamide (10 mg/kg BW) and albendazole (3, 5, 10 mg/kg BW) substances with polyvinylpyrrolidone and arabinogalactan. For the evaluation of activity of different doses of formulations against gastrointestinal tract helminths a total of 151 adult horses of the Novoaltai breed weighing 450-500 kg naturally infected with strongyles (>150 egg per gram of faeces, EPG), spp. (>20 EPG) and spp. (>10 EPG) were selected. Antiparasitic pastes were orally fed to the horses and faecal egg count reduction counts were compared prior to and 14 days after the treatment. Unassigned: Pastes with mechanically modified ivermectin showed 91.4-100% efficacy against strongyles and . Pastes with modified albendazole and niclosamide were also effective against in all tested dosages i.e. 78.6-100%. In particular, treatment with two formulations containing i) 0.2 mg ivermectin, 10 mg albendazole, 10 mg niclosamide, and ii) 0.2 mg ivermectin, 3 mg albendazole showed 100% efficacy against strongyles, and . Unassigned: Solid-phase mechanochemical technology could be applied in equine anthelminthics production. It is suggested that future studies focus on plasma concentration-time profile of these highly effective pastes.
Copyright © 2023 Marchenko et al. Published by Tehran University of Medical Sciences.
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Publication Date: 2023-05-18 PubMed ID: 37197070PubMed Central: PMC10183445DOI: 10.18502/ijpa.v18i1.12381Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers in this study have developed and tested new preparations of ivermectin, niclosamide and albendazole for treating gastrointestinal helminths in horses, using mechanochemical technology, and have found them to be highly effective.

Method of Formulation Synthesis

  • In the research, the scientists developed novel formulations of antiparasitic medicines i.e., ivermectin, niclosamide, and albendazole using solid-phase mechanochemical technology. This technology is a type of process that combines mechanical and chemical methods to create new materials or substances.
  • They combined these substances with polyvinylpyrrolidone and arabinogalactan, two types of polymers, to achieve their desired formulations. The formulations were created in the form of antiparasitic pastes.

Clinical Examination

  • The researchers evaluated the efficacy of these formulated pastes against gastrointestinal helminths in a breed of horse known as the Novoaltai breed. The horses were naturally infected with strongyles, a type of parasitic roundworm commonly found in horses, and several other types of gastrointestinal helminths.
  • The activity of different doses of formulations was evaluated by performing a fecal egg count reduction test before and after the treatment. The reduction in the number of eggs excreted in the feces was an indication of the effectiveness of the drug therapy.

Results and Findings

  • The formulations containing mechanically modified ivermectin exhibited high efficacy (between 91.4% and 100%) against strongyles and other specified helminths.
  • Formulations with modified albendazole and niclosamide also showed substantial efficacy (between 78.6% and 100%) against the parasites. Furthermore, two specific formulations that combined different amounts of the three substances showed 100% efficacy.

Conclusions and Future Directions

  • The study’s findings indicated that solid-phase mechanochemical technology could be a beneficial method for producing anthelmintics (parasitic worm treatment) for equine use.
  • The authors suggested that future research should focus on generating a plasma concentration-time profile of these highly effective pastes to understand better how they are metabolized in the body and their long-term effects.

Cite This Article

APA
Marchenko VA, Khalikov SS, Biryukov IV, Efremova EA, Ilyin MM, Sazmand A. (2023). Synthesis and Clinical Examination of Novel Formulations of Ivermectin, Albendazole and Niclosamide for the Treatment of Equine Gastrointestinal Helminthoses. Iran J Parasitol, 18(1), 66-75. https://doi.org/10.18502/ijpa.v18i1.12381

Publication

Iranian journal of parasitology
ISSN: 1735-7020
NlmUniqueID: 101464309
Country: Iran
Language: English
Volume: 18
Issue: 1
Pages: 66-75

Researcher Affiliations

Marchenko, Victor Alexeevich
  • Federal Altai Scientific Center for Agrobiotechnology, Town 35, Barnaul, Russia.
Khalikov, Salavat Samadovich
  • A.N. Nesmeyanov Institute of Organoelement Compounds Russian Academy of Science, Moscow, Russia.
Biryukov, Ivan Vladimirovich
  • Federal Altai Scientific Center for Agrobiotechnology, Town 35, Barnaul, Russia.
Efremova, Elena Alexandrovna
  • Siberian Federal Scientific Centre of Agro-Bio Technologies RAS, Krasnoobsk, Russia.
Ilyin, Mikhail Mikhailovich
  • A.N. Nesmeyanov Institute of Organoelement Compounds Russian Academy of Science, Moscow, Russia.
Sazmand, Alireza
  • Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, 6517658978 Hamedan, Iran.

Conflict of Interest Statement

Conflict of interest There was no conflict of interest to declare.

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