Synthesis of heat stress proteins in lymphocytes from livestock.
Abstract: Cultured bovine, equine, ovine and chicken lymphocytes responded to heat stress by the increased synthesis of a specific set of proteins known as heat stress proteins (HSP). Proteins with molecular weights of 70 and 90 kDa were synthesized in all species. Additional proteins were found in bovine, ovine and chicken lymphocytes. A time course of induction showed an increased synthesis of some of these proteins with only 30 min of heat stress and of several proteins with 60 min of heat stress. A specific monoclonal antibody was used to identify HSP70 as one of the stress proteins in bovine lymphocytes. Cells from these animals are capable of responding to heat stress by the production of heat stress proteins. These may be of physiological importance.
Publication Date: 1990-09-01 PubMed ID: 2211407DOI: 10.2527/1990.6892779xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the synthesis of heat stress proteins in lymphocytes from various livestock animals and concludes that these cells are capable of producing heat stress proteins when exposed to heat stress.
Animal Species and Heat Stress Proteins
- The research was conducted on cultured lymphocytes derived from bovine (cattle), equine (horse), ovine (sheep), and chicken species.
- These cells were subjected to heat stress conditions and their responses were examined.
- The heat stress condition resulted in the synthesis of specific sets of proteins known as heat stress proteins (HSP) in all the tested animal species.
- HSP with molecular weights of 70 and 90 KDa were synthesized universally across all species.
Induction Time and Additional Proteins
- The research also studied the time course of protein synthesis in response to heat stress.
- Increased synthesis of some of these proteins was observed within 30 minutes of heat stress exposure.
- Even more proteins were synthesized with 60 minutes of heat stress exposure.
- In addition to the universal heat stress proteins, additional protein types were observed in bovine, ovine, and chicken lymphocytes.
Heat Shock Protein 70 (HSP70)
- One of the notable heat shock proteins identified in bovine lymphocytes was HSP70.
- The identification was made possible using a monoclonal antibody specifically developed for HSP70.
- This suggests that HSP70 could be a key protein synthesized by livestock lymphocytes in response to heat stress.
Physiological Significance
- Heat shock proteins might play significant roles in the physiology of livestock, especially during periods of heat stress.
- The capability of livestock lymphocytes to produce heat shock proteins under heat stress conditions suggests a natural mechanism to cope with such stress.
Cite This Article
APA
Guerriero V, Raynes DA.
(1990).
Synthesis of heat stress proteins in lymphocytes from livestock.
J Anim Sci, 68(9), 2779-2783.
https://doi.org/10.2527/1990.6892779x Publication
Researcher Affiliations
- Dept. of Anim. Sci., University of Arizona, Tucson 85721.
MeSH Terms
- Animals
- Cattle
- Cells, Cultured
- Chickens
- Densitometry
- Heat-Shock Proteins / biosynthesis
- Heat-Shock Proteins / chemistry
- Horses
- Lymphocytes / metabolism
- Molecular Weight
- Sheep
Citations
This article has been cited 8 times.- Kim WS, Ghassemi Nejad J, Roh SG, Lee HG. Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves.. Animals (Basel) 2020 May 21;10(5).
- Khan A, Dou J, Wang Y, Jiang X, Khan MZ, Luo H, Usman T, Zhu H. Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells.. J Anim Sci Biotechnol 2020;11:25.
- Khan A, Khan MZ, Umer S, Khan IM, Xu H, Zhu H, Wang Y. Cellular and Molecular Adaptation of Bovine Granulosa Cells and Oocytes under Heat Stress.. Animals (Basel) 2020 Jan 9;10(1).
- Hassan FU, Nawaz A, Rehman MS, Ali MA, Dilshad SMR, Yang C. Prospects of HSP70 as a genetic marker for thermo-tolerance and immuno-modulation in animals under climate change scenario.. Anim Nutr 2019 Dec;5(4):340-350.
- Hooper HB, Dos Santos Silva P, de Oliveira SA, Merighe GKF, Negrão JA. Acute heat stress induces changes in physiological and cellular responses in Saanen goats.. Int J Biometeorol 2018 Dec;62(12):2257-2265.
- Bharati J, Dangi SS, Chouhan VS, Mishra SR, Bharti MK, Verma V, Shankar O, Yadav VP, Das K, Paul A, Bag S, Maurya VP, Singh G, Kumar P, Sarkar M. Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle.. Int J Biometeorol 2017 Jun;61(6):1017-1027.
- Kapila N, Sharma A, Kishore A, Sodhi M, Tripathi PK, Mohanty AK, Mukesh M. Impact of Heat Stress on Cellular and Transcriptional Adaptation of Mammary Epithelial Cells in Riverine Buffalo (Bubalus Bubalis).. PLoS One 2016;11(9):e0157237.
- Kishore A, Sodhi M, Kumari P, Mohanty AK, Sadana DK, Kapila N, Khate K, Shandilya U, Kataria RS, Mukesh M. Peripheral blood mononuclear cells: a potential cellular system to understand differential heat shock response across native cattle (Bos indicus), exotic cattle (Bos taurus), and riverine buffaloes (Bubalus bubalis) of India.. Cell Stress Chaperones 2014 Sep;19(5):613-21.
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