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Home / Equine Research Bank / Med Phys / Technical note: Cartilage imaging with sub-cellular resoluti...
Medical physics2023; doi: 10.1002/mp.16599

Technical note: Cartilage imaging with sub-cellular resolution using a laboratory-based phase-contrast x-ray microscope.

Abstract: Microscopic imaging of cartilage is a key tool for the study and development of treatments for osteoarthritis. When cellular and sub-cellular resolution is required, histology remains the gold standard approach, albeit limited by the lack of volumetric information as well as by processing artifacts. Cartilage imaging with the sub-cellular resolution has only been demonstrated in the synchrotron environment. Objective: To provide a proof-of-concept demonstration of the capability of a laboratory-based x-ray phase-contrast microscope to resolve sub-cellular features in a cartilage sample. Methods: This work is based on a laboratory-based x-ray microscope using intensity-modulation masks. The structured nature of the beam, resulting from the mask apertures, allows the retrieval of three contrast channels, namely, transmission, refraction and dark-field, with resolution depending only on the mask aperture width. An ex vivo equine cartilage sample was imaged with the x-ray microscope and results were validated with synchrotron tomography and histology. Results: Individual chondrocytes, that is, cells responsible for cartilage formation, could be detected with the laboratory-based microscope. The complementarity of the three retrieved contrast channels allowed the detection of sub-cellular features in the chondrocytes. Conclusions: We provide the first proof-of-concept of imaging cartilage tissue with sub-cellular resolution using a laboratory-based x-ray microscope.
© 2023 The Authors. Medical Physics published by Wiley Periodicals LLC on behalf of American Association of Physicists in Medicine.
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Publication Date: 2023-07-11 PubMed ID: 37431640DOI: 10.1002/mp.16599Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research provides a proof-of-concept demonstration that a laboratory-based x-ray phase-contrast microscope can resolve sub-cellular features in a cartilage sample, which could potentially be used to study and develop treatments for osteoarthritis.

Research Overview

  • The goal of this research is to demonstrate, via a proof-of-concept study, the ability of a laboratory-based x-ray phase-contrast microscope to detect sub-cellular features in cartilage tissue. The study addresses the limitations of current methods such as histology that are not providing adequate volumetric information and can incur processing defects.

Methodology

  • The researchers utilized an x-ray microscope, equipped with intensity-modulation masks, enabling the retrieval of three contrast channels: transmission, refraction, and dark-field. This methodology allowed them to examine these three contrast channels, with the resolution of each channel being dependent on the mask aperture width.
  • An ex vivo sample of equine cartilage was the subject of imaging for this experiment. The imaging results obtained with the laboratory-based x-ray microscope were then validated using synchrotron tomography and histology, thus confirming their reliability.

Results

  • The experiment results showed that individual chondrocytes, cells responsible for the formation of cartilage, could be detected using the laboratory-based microscope. This detection allowed for observation of sub-cellular features in the chondrocytes, which previously had only been possible in a synchrotron environment.

Conclusions

  • The researchers successfully provided a first proof-of-concept demonstration of imaging cartilage tissue with sub-cellular resolution using a laboratory-based x-ray microscope.
  • This method has potential applications in the study and development of treatments for osteoarthritis, since it presents a new way to examine the sub-cellular features of cartilage tissue, overcoming the limitations of current methods.

Cite This Article

APA
Esposito M, Astolfo A, Cipiccia S, Jones CM, Savvidis S, Ferrara JD, Endrizzi M, Dudhia J, Olivo A. (2023). Technical note: Cartilage imaging with sub-cellular resolution using a laboratory-based phase-contrast x-ray microscope. Med Phys. https://doi.org/10.1002/mp.16599

Publication

Medical physics
ISSN: 2473-4209
NlmUniqueID: 0425746
Country: United States
Language: English

Researcher Affiliations

Esposito, Michela
  • Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
Astolfo, Alberto
  • Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
Cipiccia, Silvia
  • Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
  • Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK.
Jones, Charlotte Maughan
  • Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
Savvidis, Savvas
  • Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
Ferrara, Joseph D
  • Rigaku Americas Corporation, The Woodlands, Texas, USA.
Endrizzi, Marco
  • Department of Medical Physics and Biomedical Engineering, University College London, London, UK.
Dudhia, Jayesh
  • The Royal Veterinary College, Hatfield, Hertfordshire, UK.
Olivo, Alessandro
  • Department of Medical Physics and Biomedical Engineering, University College London, London, UK.

Grant Funding

  • R01EB028829 / NIH HHS
  • R01EB028829 / NIH HHS
  • 221367/Z/20/Z / Wellcome Trust

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