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Home / Equine Research Bank / Vet Med Int / Temporal Bacteriostatic Effect and Growth Factor Loss in Equ...
Veterinary medicine international2014; 2014; 525826; doi: 10.1155/2014/525826

Temporal Bacteriostatic Effect and Growth Factor Loss in Equine Platelet Components and Plasma Cultured with Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus: A Comparative In Vitro Study.

Abstract: The aims were (1) to evaluate the bacteriostatic effect of platelet-rich plasma (PRP), platelet-rich gel (PRG), leukocyte-poor plasma (LPP), leukocyte-poor gel (LPG), plasma, and heat-inactivated plasma (IP) on both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) over a period of 24 h; (2) to determine and to compare the concentrations and degradation over time of platelet factor 4 (PF-4), transforming growth factor beta 1 (TGF-β 1), and platelet-derived growth factor isoform BB (PDGF-BB); and (3) to identify any correlations between MSSA and MRSA growth and either the cellular, PF-4, TGF-β 1, or PDGF-BB concentrations in the blood components. PRP and its byproducts from 18 horses were obtained by the tube method. All blood components were cultured with either MSSA or MRSA. Bacterial growth, PF-4, TGF-β 1, and PDGF-BB were determined at 6 h and 24 h. At six hours, bacterial growth was significantly inhibited by all blood components, with the exception of IP. MSSA was more sensitive to the treatments than MRSA. At 24 hours, bacterial growth was significantly higher in IP. MRSA bacterial growth was significantly higher in PRP, LPP, and plasma when compared to MSSA. Growth factor concentrations were not significantly affected by bacteria.
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Publication Date: 2014-11-24 PubMed ID: 25506468PubMed Central: PMC4260436DOI: 10.1155/2014/525826Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the potential of several horse blood components to inhibit the growth of two types of bacteria, methicillin-sensitive and methicillin-resistant Staphylococcus . Additionally, it observes the concentrations of certain growth factors within these components, and assesses whether the presence of bacteria affects these concentrations.

Study Objective and Methodology

  • The primary objective of the study was to determine the ability of different equine (horse) blood components, such as platelet-rich plasma (PRP), platelet-rich gel (PRG), and leukocyte-poor plasma (LPP), to inhibit the growth of two strains of Staphylococcus aureus bacteria. The strains were methicillin-sensitive (MSSA) and methicillin-resistant (MRSA).
  • The second aim was to measure and compare the concentrations of three growth factors within these blood components: platelet factor 4 (PF-4), transforming growth factor beta 1 (TGF-β 1), and platelet-derived growth factor isoform BB (PDGF-BB).
  • The research also sought to identify any correlations between the growth of MSSA and MRSA, and the cellular and growth factor concentrations in the blood components.
  • Using the tube method, PRP and associated byproducts were obtained from 18 horses. These components were then cultured with either MSSA or MRSA. The bacterial growth, as well as concentrations of PF-4, TGF-β 1, and PDGF-BB, were measured at 6 h and 24 h.

Results of the Study

  • The study found that at six hours, most equine blood components significantly inhibited bacterial growth, with the exception of heat-inactivated plasma (IP).
  • MSSA was more sensitive to the treatments than MRSA, suggesting that these blood components are more effective against this strain.
  • After 24 hours, there was a significant increase in bacterial growth within the IP.
  • Furthermore, MRSA growth was significantly higher in PRP, LPP, and plasma when compared to MSSA. This suggests that MRSA may be more resistant to the bacteriostatic effects of these components.
  • In regards to growth factor concentrations, the presence of bacteria did not result in significant effects. This implies that bacterial presence does not degrade or alter the concentrations of these growth factors.

Conclusion

  • The research provides insights into the potential of various equine blood components to inhibit bacterial growth, and the effects of bacterial presence on growth factor concentrations.
  • Such knowledge could contribute to advancements in veterinary and even human medicine, particularly in the management of infections caused by Staphylococcus aureus.

Cite This Article

APA
López C, Alvarez ME, Carmona JU. (2014). Temporal Bacteriostatic Effect and Growth Factor Loss in Equine Platelet Components and Plasma Cultured with Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus: A Comparative In Vitro Study. Vet Med Int, 2014, 525826. https://doi.org/10.1155/2014/525826

Publication

Veterinary medicine international
ISSN: 2090-8113
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2014
Pages: 525826

Researcher Affiliations

López, Catalina
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.
Alvarez, María E
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.
Carmona, Jorge U
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.

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