Testicular inhibin in the stallion: cellular source and seasonal changes in its secretion.
Abstract: The cellular localization of inhibin alpha, betaA, and betaB subunits, 3beta-hydroxysteroid dehydrogenase (3beta-HSD), and cytochrome P450 aromatase (aromatase) in stallion testes was investigated. In addition, detailed seasonal changes in circulating immunoreactive (ir)-inhibin were investigated in correlation with testosterone, estradiol, LH, and FSH. Inhibin alpha subunit-positive staining was observed in Sertoli cells, and more clearly positive staining was noted in Leydig cells. Inhibin betaA and betaB subunits were also stained in both types of cells. Immunoreactivity of 3beta-HSD and aromatase was confined to the Leydig cells. There was no seasonal effect on the percentage of the areas within seminiferous tubules and interstitial tissues that stained positive for the inhibin alpha subunit. The highest plasma concentrations of ir-inhibin were observed in the breeding season, and the lowest levels were noted during the nonbreeding season. The circulating concentrations of ir-inhibin, steroid hormones, and gonadotropins were positively correlated with each other throughout the 2 years studied. The presence of the inhibin alpha and beta subunits in Leydig cells and Sertoli cells in the equine testis suggests that these cells may secrete dimetric (bioactive) inhibin in circulation of stallions, and that the circulating ir-inhibin may be a useful indicator of the testicular function of stallions.
Publication Date: 1998-07-23 PubMed ID: 9674994DOI: 10.1095/biolreprod59.1.62Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article is about investigating the cell locations of certain inhibin subunits and enzymes in stallion testes and studying the seasonal changes of inhibin secretion, exploring the correlation between its concentration and different hormones.
Objective of the Research
- The primary objective of this research was to investigate the cellular locations of inhibin alpha, betaA, and betaB subunits, as well as 3beta-hydroxysteroid dehydrogenase (3beta-HSD), and cytochrome P450 aromatase (aromatase) in stallion testes.
- The study also sought to explore the detailed changes in the secretion of immunoreactive (ir)-inhibin over different seasons and how these changes correlate with testosterone, estradiol, LH, and FSH levels.
Findings of the Research
- Inhibin alpha subunit was found in Sertoli cells, with more clear staining observed in Leydig cells. Inhibin betaA and betaB subunits were stained in both Sertoli and Leydig cells.
- The immunoreactivity of the enzymes 3beta-HSD and aromatase was found to be confined only to Leydig cells.
- The research found no seasonal effects on the percentage of areas within seminiferous tubules and interstitial tissues staining positive for the inhibin alpha subunit.
- The highest plasma concentrations of ir-inhibin were observed during the breeding season while the lowest levels were found during the non-breeding season.
- The circulating concentrations of ir-inhibin, steroid hormones, and gonadotropins were found to be positively correlated with each other throughout the two years of the study.
Interpretations and Implications of Findings
- The presence of inhibin alpha and beta subunits in both Leydig and Sertoli cells suggests that these cells could secrete bioactive inhibin in the circulation of stallions.
- The changes in the concentration of circulating ir-inhibin could serve as a useful indicator of the testicular function in stallions, supporting its potential application in reproductive health monitoring and management of the species.
Cite This Article
APA
Nagata S, Tsunoda N, Nagamine N, Tanaka Y, Taniyama H, Nambo Y, Watanabe G, Taya K.
(1998).
Testicular inhibin in the stallion: cellular source and seasonal changes in its secretion.
Biol Reprod, 59(1), 62-68.
https://doi.org/10.1095/biolreprod59.1.62 Publication
Researcher Affiliations
- Laboratory of Racing Chemistry, Tokyo, Japan. BXD01742@niftyserve.or.jp
MeSH Terms
- 3-Hydroxysteroid Dehydrogenases / analysis
- Animals
- Aromatase / analysis
- Estradiol / blood
- Follicle Stimulating Hormone / blood
- Horses / metabolism
- Immunohistochemistry
- Inhibins / blood
- Inhibins / metabolism
- Leydig Cells / chemistry
- Luteinizing Hormone / blood
- Male
- Seasons
- Testis / chemistry
- Testis / metabolism
- Testosterone / blood
Citations
This article has been cited 15 times.- Shakeel M, Yoon M. Functions of somatic cells for spermatogenesis in stallions.. J Anim Sci Technol 2022 Jul;64(4):654-670.
- Zhou R, Wu J, Liu B, Jiang Y, Chen W, Li J, He Q, He Z. The roles and mechanisms of Leydig cells and myoid cells in regulating spermatogenesis.. Cell Mol Life Sci 2019 Jul;76(14):2681-2695.
- Chaichanathong S, Taya K, Watanabe G, Nagaoka K, Wajjwalku W, Sudsukh A, Thongtip N. Immunohistochemical localization of inhibin/activin subunits in adult Asian elephant (Elephas maximus) testes.. J Vet Med Sci 2018 Mar 30;80(3):549-552.
- Katsumata E, Jaroenporn S, Ueda Y, Arai K, Katsumata H, Watanabe G, Taya K. Circulating gonadotropins and testicular hormones during sexual maturation and annual changes in male bottlenose dolphins (Tursiops truncatus).. J Vet Med Sci 2017 Nov 17;79(11):1899-1905.
- Banco B, Grilli G, Giudice C, Marques AT, Cotti Cometti S, Visigalli G, Grieco V. Immunophenotyping of Rabbit Testicular Germ and Sertoli Cells Across Maturational Stages.. J Histochem Cytochem 2016 Nov;64(11):715-726.
- Suzuki T, Mizukami H, Nambo Y, Ishimaru M, Miyata K, Akiyama K, Korosue K, Naito H, Nagaoka K, Watanabe G, Taya K. Different effects of an extended photoperiod treatment on growth, gonadal function, and condition of hair coats in Thoroughbred yearlings reared under different climate conditions.. J Equine Sci 2015;26(4):113-24.
- Kunii H, Nambo Y, Okano A, Matsui A, Ishimaru M, Asai Y, Sato F, Fujii K, Nagaoka K, Watanabe G, Taya K. Effects of an extended photoperiod on gonadal function and condition of hair coats in Thoroughbred colts and fillies.. J Equine Sci 2015;26(2):57-66.
- Mizukami H, Suzuki T, Nambo Y, Ishimaru M, Naito H, Korosue K, Akiyama K, Miyata K, Yamanobe A, Nagaoka K, Watanabe G, Taya K. Comparison of growth and endocrine changes in Thoroughbred colts and fillies reared under different climate conditions.. J Equine Sci 2015;26(2):49-56.
- Dhakal P, Tsunoda N, Nakai R, Kitaura T, Harada T, Ito M, Nagaoka K, Toishi Y, Taniyama H, Gen W, Taya K. Annual Changes in Day-length, Temperature, and Circulating Reproductive Hormones in Thoroughbred Stallions and Geldings.. J Equine Sci 2011;22(2):29-36.
- Koyago M, Nakada K, Tsunoda N, Moriyoshi M, Sawamukai Y. Change in Morphology of Spermatozoa from Dismount Semen during the Breeding Season in Thoroughbred Stallions in Japan.. J Equine Sci 2009;20(1):1-5.
- Zhang M, Sheng X, Sun R, Li Q, Zhang H, Zhou J, Xu M, Weng Q, Watanabe G, Taya K. Seasonal changes in immunoreactivity of inhibin/activin subunits in the epididymis of wild ground squirrels (Citellus dauricus Brandt).. J Reprod Dev 2013;59(3):302-7.
- Li Q, Weng J, Zhang H, Lu L, Ma X, Wang Q, Cao H, Liu S, Xu M, Weng Q, Watanabe G, Taya K. Immunohistochemical evidence: testicular and scented glandular androgen synthesis in muskrats (Ondatra zibethicus) during the breeding season.. Eur J Histochem 2011 Oct 19;55(4):e32.
- Kim Y, Kim JS, Song MS, Seo HS, Kim JC, Bae CS, Kim S, Shin T, Kim SH, Moon C. The expression and localization of inhibin isotypes in mouse testis during postnatal development.. J Vet Sci 2008 Dec;9(4):345-9.
- Weng Q, Medan MS, Okano T, Murase T, Tsubota T, Xu M, Watanabe G, Taya K. Changes in serum inhibin levels and immunolocalization of inhibin/activin subunits during the breeding season in the wild male Japanese black bear (Ursus thibetanus japonicus).. Endocrine 2006 Apr;29(2):345-50.
- Sakurada Y, Shirota M, Inoue K, Uchida N, Shirota K. New approach to in situ quantification of ovarian gene expression in rat using a laser microdissection technique: relationship between follicle types and regulation of inhibin-alpha and cytochrome P450aromatase genes in the rat ovary.. Histochem Cell Biol 2006 Dec;126(6):735-41.
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