The application of technetium-99m hexamethylpropyleneamine oxime (99mTc-HMPAO) labeled white blood cells for the diagnosis of right dorsal ulcerative colitis in two horses.
Abstract: The application of 99mTc-HMPAO labeled white blood cells to support the diagnosis of right dorsal ulcerative colitis was studied in two horses with a history and clinical signs consistent with phenylbutazone toxicity. These images were compared to a reference horse unaffected by right dorsal ulcerative colitis. Blood was collected aseptically in heparinized syringes from the patients for in vitro white blood cell (WBC) radiolabeling. The buffy coat was separated out and radiolabeled with 99mTc-HMPAO. The radiolabeled blood was re-injected i.v. and four images of the right and left side of the patient's abdomen were acquired at 4 hours and 20 hours post-injection. Results of the nuclear study revealed no abnormal findings in the abdomen at the four-hour post-injection images in any horse. Images obtained 20 hours post-injection revealed a linear uptake of radiolabeled WBCs in the right cranioventral abdomen in the region of the right dorsal colon in both horses with right dorsal ulcerative colitis. The reference horse had no radiopharmaceutical uptake in this region. This nuclear imaging study was a rapid, non-invasive method to identify right dorsal colon inflammation. These findings not only supported the diagnosis of right dorsal ulcerative colitis, but also facilitated appropriate medical management of each horse.
Publication Date: 2000-08-24 PubMed ID: 10955501DOI: 10.1111/j.1740-8261.2000.tb02088.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research article discusses the use of radio-labeled white blood cells to diagnose a specific intestinal syndrome in horses, known as right dorsal ulcerative colitis – particularly in horses showing signs of phenylbutazone toxicity.
Objective
- The primary objective of this research was to investigate the effectiveness of using 99mTc-HMPAO labeled white blood cells as a diagnostic tool for identifying right dorsal ulcerative colitis in horses. Specifically, this application was trialed on two horses displaying signs of the condition due to phenylbutazone toxicity.
Procedure
- A sterile method was used to collect blood from the patient horses into syringes containing an anti-coagulant (heparin).
- A layer of the blood containing concentrated white blood cells, known as the buffy coat, was identified and tagged with the radioactive tracer 99mTc-HMPAO.
- The buffy coat containing the labeled white blood cells was then re-introduced into the horse’s bloodstream and allowed to distribute.
- Images were captured of the horse’s abdomen at two specific post-injection intervals – four hours and twenty hours – to track the distribution and location of the labeled white blood cells.
Results
- The study noted that there were no abnormal findings in the abdomen four hours after the buffy coat of radio-labeled white blood cells was re-injected into the affected horses.
- However, at the twenty hour interval, the images captured showed an uptake of labeled white blood cells in a specific region of the abdomen corresponding to the right dorsal colon.
- This highlighted inflammation in this part of the colon, confirming the occurrence of right dorsal ulcerative colitis.
- A reference horse, unaffected by the same type of colitis, showed no uptake of the radio-pharmaceutical tracer in the corresponding region of the abdomen.
Conclusion
- Based on the findings of this study, the researchers concluded that using technetium-99m hexamethylpropyleneamine oxime (99mTc-HMPAO) labeled white blood cells can be a swift, non-invasive method of diagnosing right dorsal ulcerative colitis in horses.
- Moreover, this study also showed that this method could potentially aid in effective medical management of the condition in affected horses.
Cite This Article
APA
East LM, Trumble TN, Steyn PF, Savage CJ, Dickinson CE, Traub-Dargatz JL.
(2000).
The application of technetium-99m hexamethylpropyleneamine oxime (99mTc-HMPAO) labeled white blood cells for the diagnosis of right dorsal ulcerative colitis in two horses.
Vet Radiol Ultrasound, 41(4), 360-364.
https://doi.org/10.1111/j.1740-8261.2000.tb02088.x Publication
Researcher Affiliations
- Department of Clinical Sciences, at College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
MeSH Terms
- Animals
- Colitis, Ulcerative / diagnosis
- Colitis, Ulcerative / diagnostic imaging
- Colitis, Ulcerative / veterinary
- Female
- Horse Diseases / diagnosis
- Horse Diseases / diagnostic imaging
- Horses
- Leukocytes
- Male
- Radionuclide Imaging / veterinary
- Technetium Tc 99m Exametazime
Citations
This article has been cited 1 times.- Malberg JA, Webb BT, Hackett ES. Colonic gastrointestinal stromal tumor resulting in recurrent colic and hematochezia in a warmblood gelding. Can Vet J 2014 May;55(5):471-4.
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