The cDNA sequences of equine antioxidative enzyme genes Cu/Zn-SOD and Mn-SOD, and these expressions in equine tissues.

The research examines the cDNA sequences of two equine antioxidative enzymes, Cu/Zn-SOD and Mn-SOD, and studies their expressions in equine tissues. The sequences were realized using RT-PCR and RACE techniques and their expressions confirmed via RT-PCR and in situ hybridization.

Determination of cDNA sequences

• The research used reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques to determine the cDNA sequences.
• The sequences were obtained from the antioxidative enzymes copper/zinc superoxide dismutase (Cu/Zn-SOD) and manganese superoxide dismutase (Mn-SOD).
• The researchers obtained the RNA used for the sequences from the testis of an adult Thoroughbred.

Findings for Cu/Zn-SOD

• They discovered that the protein coding region for equine Cu/Zn-SOD totaled 465 base pairs.
• This resulted in an estimated 154 residues of amino acids.

Findings for Mn-SOD

• For the Mn-SOD, the coding region contained a total of 669 base pairs.
• This coded for an estimated 222 residues of amino acids.

Confirmation of gene expression

• After obtaining the sequences, the researchers confirmed the expression of both Cu/Zn-SOD and Mn-SOD genes.
• This confirmation was achieved using RT-PCR and in situ hybridization techniques, and the expressions were observed in equine tissues.

In summary, the research was able to establish the cDNA sequences of equine antioxidative enzymes and determine their expressions in equine tissues. This molecular data adds to our understanding of equine genetics and these two important antioxidant enzymes.