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Veterinary journal (London, England : 1997)2011; 192(3); 345-351; doi: 10.1016/j.tvjl.2011.08.036

The comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse.

Abstract: A chondrocyte progenitor population isolated from the surface zone of articular cartilage presents a promising cell source for cell-based cartilage repair. In this study, equine articular cartilage progenitor cells (ACPCs) and equine bone marrow-derived stromal cells (BMSCs) were compared as potential cell sources for repair. Clonally derived BMSCs and ACPCs demonstrated expression of the cell fate selector gene, Notch-1, and the putative stem cell markers STRO-1, CD90 and CD166. Chondrogenic induction revealed positive labelling for collagen type II and aggrecan. Collagen type X was not detected in ACPC pellets but was observed in all BMSC pellets. In addition, it was observed that BMSCs labelled for Runx2 and matrilin-1 antibodies, whereas ACPC labelling was significantly less or absent. For both cell types, osteogenic induction revealed positive von Kossa staining in addition to positive labelling for osteocalcin. Adipogenic induction revealed a positive result via oil red O staining in both cell types. ACPCs and BMSCs have demonstrated functional equivalence in their multipotent differentiation capacity. Chondrogenic induction of BMSCs resulted in a hypertrophic cartilage (endochondral) phenotype, which can limit cartilage repair as the tissue can undergo mineralisation. ACPCs may therefore be considered superior to BMSCs in producing cartilage capable of functional repair.
Copyright © 2011 Elsevier Ltd. All rights reserved.
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Publication Date: 2011-10-02 PubMed ID: 21968294DOI: 10.1016/j.tvjl.2011.08.036Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates and compares two types of cells – equine articular cartilage progenitor cells (ACPCs) and equine bone marrow-derived stromal cells (BMSCs) – to determine their potential for use in repairing damaged cartilage in horses. The study indicated that while both cell types have multipotent differentiation capacity, ACPCs may prove superior to BMSCs in the context of cartilage repair, due to the latter’s propensity for mineralisation, which could limit the functionality of the repaired cartilage.

Overview of the Comparison

  • A specific population of progenitor cells, known as chondrocyte progenitors, located in the surface zone of articular cartilage has been isolated. These cells could be potentially useful for cartilage repair.
  • These articular cartilage progenitor cells (ACPCs), and bone marrow-derived stromal cells (BMSCs) in horses were compared in this study, examining their potential as sources for repair.

Findings from the Cell Analysis

  • BMSCs and ACPCs showed the expression of Notch-1 gene that determines the destiny of cells, and supposed stem cell markers such as STRO-1, CD90 and CD166.
  • Both cell types, after being induced for chondrogenesis, showed positive results for collagen type II and aggrecan. However, collagen type X, another widely present collagen, was found in BMSCs but not in ACPCs.
  • Following this, it was noted that BMSCs labelled for Runx2 and matrilin-1 antibodies, while ACPC labelling was significantly less or even completely absent, indicating a difference between these two cell types’ responses.

Osteogenic and Adipogenic Induction Results

  • Both the ACPCs and BMSCs demonstrated positive von Kossa staining after osteogenic induction, which indicates the presence of osteocalcin. This suggests that both these cell types could potentially differentiate into bone cells.
  • In terms of adipogenic induction, both cell types also showed positive results with oil red O staining. This suggests that both these cell types have the potential to become fat cells.

Implications for Cartilage Repair

  • While both ACPCs and BMSCs showed a similar capacity for multipotent differentiation, which is the ability of a single cell to develop into more than one type of mature cell depending on the conditions, the type of cartilage produced by these two cell types differs.
  • BMSCs ended up in a hypertrophic (or overgrown) cartilage phenotype, which can turn into bone, limiting their usefulness for cartilage repair.
  • Because of this, ACPCs may be seen as a better option for producing reparative cartilage tissue, as they do not seem to produce this bone-forming hypertrophic cartilage.

Cite This Article

APA
McCarthy HE, Bara JJ, Brakspear K, Singhrao SK, Archer CW. (2011). The comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse. Vet J, 192(3), 345-351. https://doi.org/10.1016/j.tvjl.2011.08.036

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 192
Issue: 3
Pages: 345-351

Researcher Affiliations

McCarthy, Helen E
  • School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3AX, UK. mccarthyhe1@cf.ac.uk
Bara, Jennifer J
    Brakspear, Karen
      Singhrao, Sim K
        Archer, Charles W

          MeSH Terms

          • Animals
          • Bone Marrow Cells / cytology
          • Bone Marrow Cells / physiology
          • Cartilage, Articular / cytology
          • Cartilage, Articular / physiology
          • Cell Culture Techniques
          • Cell Differentiation
          • Cell- and Tissue-Based Therapy / veterinary
          • Cells, Cultured
          • Collagen / classification
          • Collagen / metabolism
          • Gene Expression Regulation / physiology
          • Horses / physiology
          • Immunohistochemistry / veterinary
          • Stem Cells / cytology
          • Stem Cells / physiology
          • Stromal Cells / cytology
          • Stromal Cells / physiology

          Citations

          This article has been cited 84 times.
          1. Du J, Zhu Z, Liu J, Bao X, Wang Q, Shi C, Zhao C, Xu G, Li D. 3D-printed gradient scaffolds for osteochondral defects: Current status and perspectives. Int J Bioprint 2023;9(4):724.
            doi: 10.18063/ijb.724pubmed: 37323482google scholar: lookup
          2. Jiang Y, Tuan RS. Bioactivity of human adult stem cells and functional relevance of stem cell-derived extracellular matrix in chondrogenesis. Stem Cell Res Ther 2023 Jun 14;14(1):160.
            doi: 10.1186/s13287-023-03392-7pubmed: 37316923google scholar: lookup
          3. Vinod E, Parasuraman G, Lisha J J, Amirtham SM, Livingston A, Varghese JJ, Rani S, Francis DV, Rebekah G, Daniel AJ, Ramasamy B, Sathishkumar S. Human fetal cartilage-derived chondrocytes and chondroprogenitors display a greater commitment to chondrogenesis than adult cartilage resident cells. PLoS One 2023;18(4):e0285106.
            doi: 10.1371/journal.pone.0285106pubmed: 37104525google scholar: lookup
          4. Mahdavi-Jouibari F, Parseh B, Kazeminejad E, Khosravi A. Hopes and opportunities of stem cells from human exfoliated deciduous teeth (SHED) in cartilage tissue regeneration. Front Bioeng Biotechnol 2023;11:1021024.
            doi: 10.3389/fbioe.2023.1021024pubmed: 36860887google scholar: lookup
          5. Strecanska M, Danisovic L, Ziaran S, Cehakova M. The Role of Extracellular Matrix and Hydrogels in Mesenchymal Stem Cell Chondrogenesis and Cartilage Regeneration. Life (Basel) 2022 Dec 9;12(12).
            doi: 10.3390/life12122066pubmed: 36556431google scholar: lookup
          6. Chawla S, Mainardi A, Majumder N, Dönges L, Kumar B, Occhetta P, Martin I, Egloff C, Ghosh S, Bandyopadhyay A, Barbero A. Chondrocyte Hypertrophy in Osteoarthritis: Mechanistic Studies and Models for the Identification of New Therapeutic Strategies. Cells 2022 Dec 13;11(24).
            doi: 10.3390/cells11244034pubmed: 36552796google scholar: lookup
          7. Vinod E, Padmaja K, Ramasamy B, Sathishkumar S. Systematic review of articular cartilage derived chondroprogenitors for cartilage repair in animal models. J Orthop 2023 Jan;35:43-53.
            doi: 10.1016/j.jor.2022.10.012pubmed: 36387762google scholar: lookup
          8. Khan IM, McKenna J, Zhang Y. Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation. Methods Mol Biol 2023;2598:29-44.
            doi: 10.1007/978-1-0716-2839-3_4pubmed: 36355283google scholar: lookup
          9. Liu W, Feng M, Xu P. From regeneration to osteoarthritis in the knee joint: The role shift of cartilage-derived progenitor cells. Front Cell Dev Biol 2022;10:1010818.
            doi: 10.3389/fcell.2022.1010818pubmed: 36340024google scholar: lookup
          10. Desai S, Dooner M, Newberry J, Twomey-Kozak J, Molino J, Trivedi J, Patel JM, Owens BD, Jayasuriya CT. Stable human cartilage progenitor cell line stimulates healing of meniscal tears and attenuates post-traumatic osteoarthritis. Front Bioeng Biotechnol 2022;10:970235.
            doi: 10.3389/fbioe.2022.970235pubmed: 36312551google scholar: lookup
          11. Hu N, Qiu J, Xu B, Zhang S, Guo Z, Xie J, Yang W. The Role of Cartilage Stem/Progenitor Cells in Cartilage Repair in Osteoarthritis. Curr Stem Cell Res Ther 2023;18(7):892-903.
            doi: 10.2174/1574888X17666221006113739pubmed: 36201278google scholar: lookup
          12. Padmaja K, Amirtham SM, Rebekah G, Sathishkumar S, Vinod E. Supplementation of articular cartilage-derived chondroprogenitors with bone morphogenic protein-9 enhances chondrogenesis without affecting hypertrophy. Biotechnol Lett 2022 Sep;44(9):1037-1049.
            doi: 10.1007/s10529-022-03280-9pubmed: 35920961google scholar: lookup
          13. Xu W, Wang W, Liu D, Liao D. Roles of Cartilage-Resident Stem/Progenitor Cells in Cartilage Physiology, Development, Repair and Osteoarthritis. Cells 2022 Jul 27;11(15).
            doi: 10.3390/cells11152305pubmed: 35892602google scholar: lookup
          14. Gu J, Wang B, Wang T, Zhang N, Liu H, Gui J, Lu Y. Effects of Cartilage Progenitor Cells, Bone Marrow Mesenchymal Stem Cells and Chondrocytes on Cartilage Repair as Seed Cells: An in vitro Study. Drug Des Devel Ther 2022;16:1217-1230.
            doi: 10.2147/DDDT.S356936pubmed: 35509492google scholar: lookup
          15. Johnson NN, Amirtham SM, Sandya Rani B, Sathishkumar S, Rebekah G, Vinod E. Assessment of the inherent chondrogenic potential of human articular cartilage-derived chondroprogenitors in pellet culture using a novel whole pellet processing approach. J Orthop 2022 May-Jun;31:45-51.
            doi: 10.1016/j.jor.2022.03.007pubmed: 35368732google scholar: lookup
          16. Shen X, Zhu T, Xue J, Zhang Y, Lu Y, Yang H, Yu Z, Zhu Y, Zhu X. Influence of bFGF on in vitro expansion and chondrogenic construction of articular cartilage-derived progenitor cells. Ann Transl Med 2022 Jan;10(2):36.
            doi: 10.21037/atm-21-5604pubmed: 35282057google scholar: lookup
          17. Rikkers M, Korpershoek JV, Levato R, Malda J, Vonk LA. The clinical potential of articular cartilage-derived progenitor cells: a systematic review. NPJ Regen Med 2022 Jan 10;7(1):2.
            doi: 10.1038/s41536-021-00203-6pubmed: 35013329google scholar: lookup
          18. Vinod E, Johnson NN, Kumar S, Amirtham SM, James JV, Livingston A, Rebekah G, Daniel AJ, Ramasamy B, Sathishkumar S. Migratory chondroprogenitors retain superior intrinsic chondrogenic potential for regenerative cartilage repair as compared to human fibronectin derived chondroprogenitors. Sci Rep 2021 Dec 8;11(1):23685.
            doi: 10.1038/s41598-021-03082-5pubmed: 34880351google scholar: lookup
          19. Rikkers M, Korpershoek JV, Levato R, Malda J, Vonk LA. Progenitor Cells in Healthy and Osteoarthritic Human Cartilage Have Extensive Culture Expansion Capacity while Retaining Chondrogenic Properties. Cartilage 2021 Dec;13(2_suppl):129S-142S.
            doi: 10.1177/19476035211059600pubmed: 34802263google scholar: lookup
          20. Vinod E, Padmaja K, Livingston A, James JV, Amirtham SM, Sathishkumar S, Ramasamy B, Rebekah G, Daniel AJ, Kachroo U. Prospective Isolation and Characterization of Chondroprogenitors from Human Chondrocytes Based on CD166/CD34/CD146 Surface Markers. Cartilage 2021 Dec;13(2_suppl):808S-817S.
            doi: 10.1177/19476035211042412pubmed: 34528493google scholar: lookup
          21. de Souza JB, Rosa GDS, Rossi MC, Stievani FC, Pfeifer JPH, Krieck AMT, Bovolato ALC, Fonseca-Alves CE, Borrás VA, Alves ALG. In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery. Front Bioeng Biotechnol 2021;9:674581.
            doi: 10.3389/fbioe.2021.674581pubmed: 34513806google scholar: lookup
          22. Korpershoek JV, Rikkers M, de Windt TS, Tryfonidou MA, Saris DBF, Vonk LA. Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin: A Novel Approach in Meniscus Tissue Engineering. Int J Mol Sci 2021 Aug 10;22(16).
            doi: 10.3390/ijms22168614pubmed: 34445320google scholar: lookup
          23. Mok CH, MacLeod JN. Kinetics of Gene Expression Changes in Equine Fetal Interzone and Anlagen Cells Over 14 Days of Induced Chondrogenesis. Front Vet Sci 2021;8:722324.
            doi: 10.3389/fvets.2021.722324pubmed: 34434986google scholar: lookup
          24. Ramasamy TS, Yee YM, Khan IM. Chondrocyte Aging: The Molecular Determinants and Therapeutic Opportunities. Front Cell Dev Biol 2021;9:625497.
            doi: 10.3389/fcell.2021.625497pubmed: 34336816google scholar: lookup
          25. Fülber J, Agreste FR, Seidel SRT, Sotelo EDP, Barbosa ÂP, Michelacci YM, Baccarin RYA. Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system. World J Stem Cells 2021 Jun 26;13(6):645-658.
            doi: 10.4252/wjsc.v13.i6.645pubmed: 34249233google scholar: lookup
          26. Chen CH, Tang N, Xue K, Zhang HZ, Chen YH, Xu P, Sun K, Tao K, Liu K. Long-Term Tri-Modal In Vivo Tracking of Engrafted Cartilage-Derived Stem/Progenitor Cells Based on Upconversion Nanoparticles. Biomolecules 2021 Jun 29;11(7).
            doi: 10.3390/biom11070958pubmed: 34209859google scholar: lookup
          27. Hu H, Liu W, Sun C, Wang Q, Yang W, Zhang Z, Xia Z, Shao Z, Wang B. Endogenous Repair and Regeneration of Injured Articular Cartilage: A Challenging but Promising Therapeutic Strategy. Aging Dis 2021 Jun;12(3):886-901.
            doi: 10.14336/AD.2020.0902pubmed: 34094649google scholar: lookup
          28. Vinod E, Kachroo U, Rebekah G, Thomas S, Ramasamy B. In vitro chondrogenic differentiation of human articular cartilage derived chondroprogenitors using pulsed electromagnetic field. J Clin Orthop Trauma 2021 Mar;14:22-28.
            doi: 10.1016/j.jcot.2020.09.034pubmed: 33717892google scholar: lookup
          29. Schmidt S, Abinzano F, Mensinga A, Teßmar J, Groll J, Malda J, Levato R, Blunk T. Differential Production of Cartilage ECM in 3D Agarose Constructs by Equine Articular Cartilage Progenitor Cells and Mesenchymal Stromal Cells. Int J Mol Sci 2020 Sep 25;21(19).
            doi: 10.3390/ijms21197071pubmed: 32992847google scholar: lookup
          30. Vinod E, Parameswaran R, Ramasamy B, Kachroo U. Pondering the Potential of Hyaline Cartilage-Derived Chondroprogenitors for Tissue Regeneration: A Systematic Review. Cartilage 2021 Dec;13(2_suppl):34S-52S.
            doi: 10.1177/1947603520951631pubmed: 32840123google scholar: lookup
          31. Twomey-Kozak J, Desai S, Liu W, Li NY, Lemme N, Chen Q, Owens BD, Jayasuriya CT. Distal-Less Homeobox 5 Is a Therapeutic Target for Attenuating Hypertrophy and Apoptosis of Mesenchymal Progenitor Cells. Int J Mol Sci 2020 Jul 8;21(14).
            doi: 10.3390/ijms21144823pubmed: 32650430google scholar: lookup
          32. Jessop ZM, Al-Sabah A, Simoes IN, Burnell SEA, Pieper IL, Thornton CA, Whitaker IS. Isolation and characterisation of nasoseptal cartilage stem/progenitor cells and their role in the chondrogenic niche. Stem Cell Res Ther 2020 May 14;11(1):177.
            doi: 10.1186/s13287-020-01663-1pubmed: 32408888google scholar: lookup
          33. Kachroo U, Zachariah SM, Thambaiah A, Tabasum A, Livingston A, Rebekah G, Srivastava A, Vinod E. Comparison of Human Platelet Lysate versus Fetal Bovine Serum for Expansion of Human Articular Cartilage-Derived Chondroprogenitors. Cartilage 2021 Dec;13(2_suppl):107S-116S.
            doi: 10.1177/1947603520918635pubmed: 32406256google scholar: lookup
          34. Morgan BJ, Bauza-Mayol G, Gardner OFW, Zhang Y, Levato R, Archer CW, van Weeren R, Malda J, Conlan RS, Francis LW, Khan IM. Bone Morphogenetic Protein-9 Is a Potent Chondrogenic and Morphogenic Factor for Articular Cartilage Chondroprogenitors. Stem Cells Dev 2020 Jul;29(14):882-894.
            doi: 10.1089/scd.2019.0209pubmed: 32364057google scholar: lookup
          35. Grandi FC, Baskar R, Smeriglio P, Murkherjee S, Indelli PF, Amanatullah DF, Goodman S, Chu C, Bendall S, Bhutani N. Single-cell mass cytometry reveals cross-talk between inflammation-dampening and inflammation-amplifying cells in osteoarthritic cartilage. Sci Adv 2020 Mar;6(11):eaay5352.
            doi: 10.1126/sciadv.aay5352pubmed: 32201724google scholar: lookup
          36. Rubí-Sans G, Recha-Sancho L, Pérez-Amodio S, Mateos-Timoneda MÁ, Semino CE, Engel E. Development of a Three-Dimensional Bioengineered Platform for Articular Cartilage Regeneration. Biomolecules 2019 Dec 28;10(1).
            doi: 10.3390/biom10010052pubmed: 31905668google scholar: lookup
          37. Contentin R, Demoor M, Concari M, Desancé M, Audigié F, Branly T, Galéra P. Comparison of the Chondrogenic Potential of Mesenchymal Stem Cells Derived from Bone Marrow and Umbilical Cord Blood Intended for Cartilage Tissue Engineering. Stem Cell Rev Rep 2020 Feb;16(1):126-143.
            doi: 10.1007/s12015-019-09914-2pubmed: 31745710google scholar: lookup
          38. Newberry J, Desai S, Adler C, Li N, Karamchedu NP, Fleming BC, Jayasuriya CT. SDF-1 preconditioned HPC scaffolds mobilize cartilage-derived progenitors and stimulate meniscal fibrocartilage repair in human explant tissue culture. Connect Tissue Res 2020 May-Jul;61(3-4):338-348.
            doi: 10.1080/03008207.2019.1689966pubmed: 31744353google scholar: lookup
          39. Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates. Front Bioeng Biotechnol 2019;7:155.
            doi: 10.3389/fbioe.2019.00155pubmed: 31355191google scholar: lookup
          40. Matta C, Boocock DJ, Fellows CR, Miosge N, Dixon JE, Liddell S, Smith J, Mobasheri A. Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis. Sci Rep 2019 Jun 21;9(1):9018.
            doi: 10.1038/s41598-019-44957-ypubmed: 31227739google scholar: lookup
          41. Si H, Liang M, Cheng J, Shen B. [Effects of cartilage progenitor cells and microRNA-140 on repair of osteoarthritic cartilage injury]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2019 May 15;33(5):650-658.
            doi: 10.7507/1002-1892.201806060pubmed: 31090363google scholar: lookup
          42. Vinod E, James JV, Sabareeswaran A, Amirtham SM, Thomas G, Sathishkumar S, Ozbey O, Boopalan PRJVC. Intraarticular injection of allogenic chondroprogenitors for treatment of osteoarthritis in rabbit knee model. J Clin Orthop Trauma 2019 Jan-Feb;10(1):16-23.
            doi: 10.1016/j.jcot.2018.07.003pubmed: 30705526google scholar: lookup
          43. Pattappa G, Johnstone B, Zellner J, Docheva D, Angele P. The Importance of Physioxia in Mesenchymal Stem Cell Chondrogenesis and the Mechanisms Controlling Its Response. Int J Mol Sci 2019 Jan 23;20(3).
            doi: 10.3390/ijms20030484pubmed: 30678074google scholar: lookup
          44. Boere J, Malda J, van de Lest CHA, van Weeren PR, Wauben MHM. Extracellular Vesicles in Joint Disease and Therapy. Front Immunol 2018;9:2575.
            doi: 10.3389/fimmu.2018.02575pubmed: 30483255google scholar: lookup
          45. Wu Y, Kennedy P, Bonazza N, Yu Y, Dhawan A, Ozbolat I. Three-Dimensional Bioprinting of Articular Cartilage: A Systematic Review. Cartilage 2021 Jan;12(1):76-92.
            doi: 10.1177/1947603518809410pubmed: 30373384google scholar: lookup
          46. Martin-Pena A, Porter RM, Plumton G, McCarrel TM, Morton AJ, Guijarro MV, Ghivizzani SC, Sharma B, Palmer GD. Lentiviral-based reporter constructs for profiling chondrogenic activity in primary equine cell populations. Eur Cell Mater 2018 Oct 12;36:156-170.
            doi: 10.22203/eCM.v036a12pubmed: 30311630google scholar: lookup
          47. Baird A, Lindsay T, Everett A, Iyemere V, Paterson YZ, McClellan A, Henson FMD, Guest DJ. Osteoblast differentiation of equine induced pluripotent stem cells. Biol Open 2018 May 10;7(5).
            doi: 10.1242/bio.033514pubmed: 29685993google scholar: lookup
          48. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
            doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
          49. He R, Wang B, Cui M, Xiong Z, Lin H, Zhao L, Li Z, Wang Z, Peggrem S, Xia Z, Shao Z. Link Protein N-Terminal Peptide as a Potential Stimulating Factor for Stem Cell-Based Cartilage Regeneration. Stem Cells Int 2018;2018:3217895.
            doi: 10.1155/2018/3217895pubmed: 29531532google scholar: lookup
          50. Gurruchaga H, Saenz Del Burgo L, Garate A, Delgado D, Sanchez P, Orive G, Ciriza J, Sanchez M, Pedraz JL. Cryopreservation of Human Mesenchymal Stem Cells in an Allogeneic Bioscaffold based on Platelet Rich Plasma and Synovial Fluid. Sci Rep 2017 Nov 16;7(1):15733.
            doi: 10.1038/s41598-017-16134-6pubmed: 29146943google scholar: lookup
          51. Vinod E, Boopalan PRJVC, Sathishkumar S. Reserve or Resident Progenitors in Cartilage? Comparative Analysis of Chondrocytes versus Chondroprogenitors and Their Role in Cartilage Repair. Cartilage 2018 Apr;9(2):171-182.
            doi: 10.1177/1947603517736108pubmed: 29047310google scholar: lookup
          52. Levato R, Webb WR, Otto IA, Mensinga A, Zhang Y, van Rijen M, van Weeren R, Khan IM, Malda J. The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells. Acta Biomater 2017 Oct 1;61:41-53.
            doi: 10.1016/j.actbio.2017.08.005pubmed: 28782725google scholar: lookup
          53. Huang YZ, Xie HQ, Silini A, Parolini O, Zhang Y, Deng L, Huang YC. Mesenchymal Stem/Progenitor Cells Derived from Articular Cartilage, Synovial Membrane and Synovial Fluid for Cartilage Regeneration: Current Status and Future Perspectives. Stem Cell Rev Rep 2017 Oct;13(5):575-586.
            doi: 10.1007/s12015-017-9753-1pubmed: 28721683google scholar: lookup
          54. Anderson DE, Markway BD, Weekes KJ, McCarthy HE, Johnstone B. Physioxia Promotes the Articular Chondrocyte-Like Phenotype in Human Chondroprogenitor-Derived Self-Organized Tissue. Tissue Eng Part A 2018 Feb;24(3-4):264-274.
            doi: 10.1089/ten.TEA.2016.0510pubmed: 28474537google scholar: lookup
          55. Cao Y, Gang X, Sun C, Wang G. Mesenchymal Stem Cells Improve Healing of Diabetic Foot Ulcer. J Diabetes Res 2017;2017:9328347.
            doi: 10.1155/2017/9328347pubmed: 28386568google scholar: lookup
          56. Wang M, Yuan Z, Ma N, Hao C, Guo W, Zou G, Zhang Y, Chen M, Gao S, Peng J, Wang A, Wang Y, Sui X, Xu W, Lu S, Liu S, Guo Q. Advances and Prospects in Stem Cells for Cartilage Regeneration. Stem Cells Int 2017;2017:4130607.
            doi: 10.1155/2017/4130607pubmed: 28246531google scholar: lookup
          57. Fellows CR, Williams R, Davies IR, Gohil K, Baird DM, Fairclough J, Rooney P, Archer CW, Khan IM. Characterisation of a divergent progenitor cell sub-populations in human osteoarthritic cartilage: the role of telomere erosion and replicative senescence. Sci Rep 2017 Feb 2;7:41421.
            doi: 10.1038/srep41421pubmed: 28150695google scholar: lookup
          58. Zayed M, Caniglia C, Misk N, Dhar MS. Donor-Matched Comparison of Chondrogenic Potential of Equine Bone Marrow- and Synovial Fluid-Derived Mesenchymal Stem Cells: Implications for Cartilage Tissue Regeneration. Front Vet Sci 2016;3:121.
            doi: 10.3389/fvets.2016.00121pubmed: 28149840google scholar: lookup
          59. Fellows CR, Matta C, Zakany R, Khan IM, Mobasheri A. Adipose, Bone Marrow and Synovial Joint-Derived Mesenchymal Stem Cells for Cartilage Repair. Front Genet 2016;7:213.
            doi: 10.3389/fgene.2016.00213pubmed: 28066501google scholar: lookup
          60. Chen Y, Chen Y, Zhang S, Du X, Bai B. Parathyroid Hormone-Induced Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation and its Repair of Articular Cartilage Injury in Rabbits. Med Sci Monit Basic Res 2016 Nov 16;22:132-145.
            doi: 10.12659/msmbr.900242pubmed: 27847384google scholar: lookup
          61. Anderson DE, Markway BD, Bond D, McCarthy HE, Johnstone B. Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity. Stem Cell Res Ther 2016 Oct 20;7(1):154.
            doi: 10.1186/s13287-016-0419-8pubmed: 27765063google scholar: lookup
          62. Jayasuriya CT, Chen Y, Liu W, Chen Q. The influence of tissue microenvironment on stem cell-based cartilage repair. Ann N Y Acad Sci 2016 Nov;1383(1):21-33.
            doi: 10.1111/nyas.13170pubmed: 27464254google scholar: lookup
          63. Angele P, Kujat R, Koch M, Zellner J. Role of mesenchymal stem cells in meniscal repair. J Exp Orthop 2014 Dec;1(1):12.
            doi: 10.1186/s40634-014-0012-ypubmed: 26914757google scholar: lookup
          64. Neumann AJ, Gardner OF, Williams R, Alini M, Archer CW, Stoddart MJ. Human Articular Cartilage Progenitor Cells Are Responsive to Mechanical Stimulation and Adenoviral-Mediated Overexpression of Bone-Morphogenetic Protein 2. PLoS One 2015;10(8):e0136229.
            doi: 10.1371/journal.pone.0136229pubmed: 26292283google scholar: lookup
          65. Jayasuriya CT, Chen Q. Potential benefits and limitations of utilizing chondroprogenitors in cell-based cartilage therapy. Connect Tissue Res 2015;56(4):265-71.
            doi: 10.3109/03008207.2015.1040547pubmed: 26075411google scholar: lookup
          66. Nelson L, McCarthy HE, Fairclough J, Williams R, Archer CW. Evidence of a Viable Pool of Stem Cells within Human Osteoarthritic Cartilage. Cartilage 2014 Oct;5(4):203-14.
            doi: 10.1177/1947603514544953pubmed: 26069699google scholar: lookup
          67. Chen S, Fu P, Cong R, Wu H, Pei M. Strategies to minimize hypertrophy in cartilage engineering and regeneration. Genes Dis 2015 Mar 1;2(1):76-95.
            doi: 10.1016/j.gendis.2014.12.003pubmed: 26000333google scholar: lookup
          68. Jiang Y, Tuan RS. Origin and function of cartilage stem/progenitor cells in osteoarthritis. Nat Rev Rheumatol 2015 Apr;11(4):206-12.
            doi: 10.1038/nrrheum.2014.200pubmed: 25536487google scholar: lookup
          69. Mazor M, Lespessailles E, Coursier R, Daniellou R, Best TM, Toumi H. Mesenchymal stem-cell potential in cartilage repair: an update. J Cell Mol Med 2014 Dec;18(12):2340-50.
            doi: 10.1111/jcmm.12378pubmed: 25353372google scholar: lookup
          70. Candela ME, Yasuhara R, Iwamoto M, Enomoto-Iwamoto M. Resident mesenchymal progenitors of articular cartilage. Matrix Biol 2014 Oct;39:44-9.
            doi: 10.1016/j.matbio.2014.08.015pubmed: 25179676google scholar: lookup
          71. Löfgren M, Ekman S, Svala E, Lindahl A, Ley C, Skiöldebrand E. Cell and matrix modulation in prenatal and postnatal equine growth cartilage, zones of Ranvier and articular cartilage. J Anat 2014 Nov;225(5):548-68.
            doi: 10.1111/joa.12232pubmed: 25175365google scholar: lookup
          72. Somoza RA, Welter JF, Correa D, Caplan AI. Chondrogenic differentiation of mesenchymal stem cells: challenges and unfulfilled expectations. Tissue Eng Part B Rev 2014 Dec;20(6):596-608.
            doi: 10.1089/ten.TEB.2013.0771pubmed: 24749845google scholar: lookup
          73. Anderson JA, Little D, Toth AP, Moorman CT 3rd, Tucker BS, Ciccotti MG, Guilak F. Stem cell therapies for knee cartilage repair: the current status of preclinical and clinical studies. Am J Sports Med 2014 Sep;42(9):2253-61.
            doi: 10.1177/0363546513508744pubmed: 24220016google scholar: lookup
          74. Shepard JB, Jeong JW, Maihle NJ, O'Brien S, Dealy CN. Transient anabolic effects accompany epidermal growth factor receptor signal activation in articular cartilage in vivo. Arthritis Res Ther 2013;15(3):R60.
            doi: 10.1186/ar4233pubmed: 23705804google scholar: lookup
          75. John JL, Singh G, Parasuraman G, Livingston A, Rebekah G, Daniel AJ, Sathishkumar S, Vinod E. Comparative assessment of human chondroprogenitor viability and molecular phenotype in various parenteral transport media. Histochem Cell Biol 2025 Aug 20;163(1):82.
            doi: 10.1007/s00418-025-02416-ypubmed: 40833641google scholar: lookup
          76. Lu J, Gao Y, Cao C, Wang H, Ruan Y, Qin K, Liu H, Wang Y, Yang P, Liu Y, Ma Y, Yu Z, Wang Y, Zhong Z, Chang F. 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications. Mater Today Bio 2025 Jun;32:101834.
            doi: 10.1016/j.mtbio.2025.101834pubmed: 40487176google scholar: lookup
          77. Esa A, Ahmed N, Elsheikh MF, Ahmed H, Cherif RA, Archer C. Isolation and Analysis of Matched Osteoarthritic Cartilage Progenitor Cells and Bone Marrow Mesenchymal Stem Cells. Cureus 2025 Mar;17(3):e80844.
            doi: 10.7759/cureus.80844pubmed: 40255792google scholar: lookup
          78. Vinod E, Parasuraman G, J JL, Varghese JJ, Livingston A, Rebekah G, Francis DV, Sathishkumar S, Daniel AJ, Ramasamy B. Comparative assessment of chondral defect repair using human bone marrow- and adipose tissue-derived mesenchymal stem cells, adult and foetal articular cartilage-derived chondrocytes, and chondroprogenitors: an ex-vivo model. Biotechnol Lett 2025 Jan 8;47(1):17.
            doi: 10.1007/s10529-024-03558-0pubmed: 39775982google scholar: lookup
          79. Betensky DJ, Chen MD, Trivedi J, Desai S, Twomey-Kozak J, Wen S, Jayasuriya CT. Extracellular vesicles from cartilage progenitors stimulate type II collagen expression and wound healing in meniscal cells. J Orthop Res 2025 Mar;43(3):682-691.
            doi: 10.1002/jor.26013pubmed: 39511943google scholar: lookup
          80. Parasuraman G, Amirtham SM, Francis DV, Livingston A, Ramasamy B, Sathishkumar S, Vinod E. Evaluation of Chondral Defect Repair Using Human Fibronectin Adhesion Assay-Derived Chondroprogenitors Suspended in Lyophilized Fetal Collagen Scaffold: An Ex Vivo Osteochondral Unit Model Study. Indian J Orthop 2024 Aug;58(8):991-1000.
            doi: 10.1007/s43465-024-01192-6pubmed: 39087036google scholar: lookup
          81. Yang DS, Trivedi J, Betensky D, Desai S, Owens BD, Jayasuriya CT. Kartogenin Induces Chondrogenesis in Cartilage Progenitor Cells and Attenuates Cell Hypertrophy in Marrow-Derived Stromal Cells. Curr Stem Cell Res Ther 2025;20(5):577-583.
            doi: 10.2174/011574888X314971240511151616pubmed: 38778613google scholar: lookup
          82. Shigley C, Trivedi J, Meghani O, Owens BD, Jayasuriya CT. Suppressing Chondrocyte Hypertrophy to Build Better Cartilage. Bioengineering (Basel) 2023 Jun 20;10(6).
            doi: 10.3390/bioengineering10060741pubmed: 37370672google scholar: lookup
          83. Vinod E, Lisha J J, Parasuraman G, Livingston A, Daniel AJ, Sathishkumar S. Evaluation of ghrelin as a distinguishing marker for human articular cartilage-derived chondrocytes and chondroprogenitors. J Clin Orthop Trauma 2023 Jun;41:102175.
            doi: 10.1016/j.jcot.2023.102175pubmed: 37303495google scholar: lookup
          84. Peng Y, Li J, Lin H, Tian S, Liu S, Pu F, Zhao L, Ma K, Qing X, Shao Z. Endogenous repair theory enriches construction strategies for orthopaedic biomaterials: a narrative review. Biomater Transl 2021;2(4):343-360.
            doi: 10.12336/biomatertransl.2021.04.008pubmed: 35837417google scholar: lookup
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