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Home / Equine Research Bank / Vet Res Commun / The complete ITS2 barcoding region for Strongylus vulgaris a...
Veterinary research communications2023; 47(3); 1767-1771; doi: 10.1007/s11259-022-10067-w

The complete ITS2 barcoding region for Strongylus vulgaris and Strongylus edentatus.

Abstract: Gastrointestinal nematode parasites are of major concern for horses, where Strongylus vulgaris is considered the most pathogenic among the Strongylus species. Diagnosis of S. vulgaris infections can be determined with next generation sequencing techniques, which are inherently dependent on reference sequences. The best marker for parasitic nematodes is internal transcribed spacer 2 (ITS2) and we provide the first complete ITS2 sequences from five morphologically identified S. vulgaris and additional sequences from two S. edentatus. These sequences have high similarity to already published partial sequences and amplicon sequence variants (ASV) based on next generation sequencing (NGS). The ITS2 sequences from S. vulgaris matched available partial ITS2 sequences and the full ASVs, whereas the S. edentatus sequence matched another complete sequence. We also compare Sanger sequencing and NGS methods and conclude that the ITS2 variation is better represented with NGS methods. Based on this, we recommend that further sequencing of morphologically identified specimens of various species should be performed with NGS cover the intraspecific variation in the ITS2.
© 2023. The Author(s).
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Publication Date: 2023-01-04 PubMed ID: 36598645PubMed Central: PMC10485102DOI: 10.1007/s11259-022-10067-wGoogle Scholar: Lookup
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  • Journal Article

Summary

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The research article focuses on determining the best techniques for diagnosing gastrointestinal nematode parasites, particularly Strongylus vulgaris and Strongylus edentatus in horses. The researchers provide first of its kind comprehensive sequences of internal transcribed spacer 2 (ITS2), the most reliable marker for these parasites.

Background

  • Gastrointestinal nematode parasites present a significant health concern for horses, and out of all Strongylus species, Strongylus vulgaris is most pathological.
  • The diagnosis of S. vulgaris infections largely depends on next-generation sequencing techniques, which need reference sequences for accurate determination.
  • The internal transcribed spacer 2 (ITS2) is a very effective marker for tracking parasitic nematodes.

Research Findings

  • The authors offer the first comprehensive ITS2 sequences obtained from five morphologically identified S. vulgaris and some additional sequences from two S. edentatus.
  • These sequences were found to have high similarity to already published partial sequences and next-generation sequencing (NGS) derived amplicon sequence variants (ASV).
  • The ITS2 sequences from S. vulgaris were found to match the available partial ITS2 sequences and the complete ASVs, while the S. edentatus sequence matched another complete sequence.

Comparison of Sanger Sequencing and NGS

  • The researchers also conducted a comparative study between Sanger sequencing and modern NGS methods.
  • They concluded that ITS2 variation is represented better with NGS methodologies.

Recommendations

  • The study ends recommending that future sequencing of morphologically identified specimens across varying species should be carried out with NGS, as it effectively covers the intraspecific variation in ITS2.

Cite This Article

APA
Halvarsson P, Tydén E. (2023). The complete ITS2 barcoding region for Strongylus vulgaris and Strongylus edentatus. Vet Res Commun, 47(3), 1767-1771. https://doi.org/10.1007/s11259-022-10067-w

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 47
Issue: 3
Pages: 1767-1771

Researcher Affiliations

Halvarsson, Peter
  • Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, PO Box 7036, 750 05, Uppsala, Sweden. peter.halvarsson@slu.se.
Tydén, Eva
  • Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, PO Box 7036, 750 05, Uppsala, Sweden.

MeSH Terms

  • Animals
  • Horses / genetics
  • Strongylus
  • Intestinal Diseases, Parasitic / veterinary
  • Horse Diseases

Grant Funding

  • H-15-47-097 / Foundation for Swedish and Norwegian Equine Research

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 1 times.
  1. Mason B, Cervena B, Frias L, Goossens B, Hasegawa H, Keuk K, Langgeng A, Majewski K, Matsumoto T, Matsuura K, Mendonça R, Okamoto M, Peter S, Petrzelkova KJ, Sipangkui S, Xu Z, Pafco B, MacIntosh AJJ. Novel insight into the genetic diversity of strongylid nematodes infecting South-East and East Asian primates. Parasitology 2024 Apr;151(5):514-522.
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