The complete mitochondrial genome of Oxyuris equi: Comparison with other closely related species and phylogenetic implications.
Abstract: The equine pinworm Oxyuris equi (Nematoda: Oxyuridomorpha) is the most common horse nematode, has a worldwide distribution, and causes major economic losses. In the present study, the complete O. equi mitochondrial (mt) genome was sequenced, and the mt genome structure and organization were compared with those of other closely related pinworm species, Enterobius vermicularis and Wellcomia siamensis. The O. equi mt genome is a 13,641-bp circular DNA molecule that encodes 36 genes (12 protein-coding genes, 22 tRNAs, and two rRNAs) and one non-coding region, which is slightly shorter than that of E. vermicularis and W. siamensis. The O. equi mt gene arrangement was consistent with that of GA13-type E. vermicularis but it differs from GA12-type W. siamensis. Phylogenetic analyses using concatenated amino acid sequences of the 12 protein-coding genes with three different computational algorithms (maximum parsimony, maximum likelihood, and Bayesian inference) revealed that there were two distinct clades in Chromadorea nematodes that reflected infraorder. Spiruromorpha formed one clade, whereas Rhabditomorpha, Ascaridomorpha, and Oxyuridomorpha formed another clade. O. equi, E. vermicularis, and W. siamensis represent distinct but closely related species, which indicated that Oxyuridomorpha is paraphyletic. Sequencing the O. equi mt genome provides novel genetic markers for studying the molecular epidemiology and population genetics of pinworms.
Copyright © 2015 Elsevier Inc. All rights reserved.
Publication Date: 2015-10-09 PubMed ID: 26452611DOI: 10.1016/j.exppara.2015.09.013Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers conducted a comprehensive study of the mitochondrial genome of the equine pinworm, Oxyuris equi. They compared this genome structure with those of other pinworm species, highlighting genetic similarities and differences which contribute to our understanding of pinworm evolution.
Study Overview
- This research focused on the complete mitochondrial genome of the equine pinworm, Oxyuris equi, which is regarded as the most prevalent horse nematode causing major economic losses.
- The researchers sequenced the pinworm’s mitochondrial (mt) genome and compared it to those of related pinworm species (Enterobius vermicularis and Wellcomia siamensis).
Results: Genome Structure
- The O. equi’s mitochondrial genome is a 13,641-bp circular DNA molecule that encodes 36 genes (12 protein-coding genes, 22 tRNAs, two rRNAs), and one non-coding region.
- Compared to E. vermicularis and W. siamensis, O. equi’s genome is slightly shorter.
- The gene arrangement observed was consistent with that of GA13-type E. vermicularis, but differed from GA12-type W. siamensis.
Phylogenetic Analysis
- Researchers used maximum parsimony, maximum likelihood, and Bayesian inference computational algorithms to conduct phylogenetic analysis using concatenated amino acid sequences of the 12 protein-coding genes.
- Within the family of Chromadorea nematodes, two distinct clades were identified. One clade was formed by Spiruromorpha, while Rhabditomorpha, Ascaridomorpha, and Oxyuridomorpha formed another clade.
- O. equi, E. vermicularis, and W. siamensis were found to represent distinct but closely related species, thus indicating that Oxyuridomorpha is paraphyletic.
Implications
- The completion of the O. equi mitochondrial genome sequencing contributes to studies on the molecular epidemiology and population genetics of pinworms – it provides new genetic markers for this purpose.
- The results illuminate the evolutional relationship between different types of nematodes, providing valuable insights for further research on the control and prevention of diseases caused by these parasites.
Cite This Article
APA
Zhang Y, Xu WW, Guo DH, Liu ZX, Duan H, Su X, Fu X, Yue DM, Gao Y, Wang CR.
(2015).
The complete mitochondrial genome of Oxyuris equi: Comparison with other closely related species and phylogenetic implications.
Exp Parasitol, 159, 215-221.
https://doi.org/10.1016/j.exppara.2015.09.013 Publication
Researcher Affiliations
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: zhangyan1825@163.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: 516330711@qq.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: 423239958@qq.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: 712377765@qq.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: 604907499@qq.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: 1984546659@qq.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: 421096250@qq.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: dongmeiyue@163.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: gaoyuangy89@126.com.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, PR China. Electronic address: Chunren@sohu.com.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA, Mitochondrial / chemistry
- Genetic Markers
- Genome, Mitochondrial / genetics
- Helminth Proteins / genetics
- Horse Diseases / parasitology
- Horses
- Molecular Sequence Data
- Oxyuriasis / parasitology
- Oxyuriasis / veterinary
- Oxyuroidea / classification
- Oxyuroidea / genetics
- Phylogeny
- RNA, Ribosomal / genetics
- RNA, Transfer / genetics
- Sequence Alignment
- Sequence Analysis
Citations
This article has been cited 2 times.- Huo C, Bao F, Long H, Qin T, Zhang S. The complete mitochondrial genome of Wellcomia compar (Spirurina: Oxyuridae) and its genome characterization and phylogenetic analysis. Sci Rep 2023 Sep 2;13(1):14426.
- Gao Y, Zhang Y, Yang X, Qiu JH, Duan H, Xu WW, Chang QC, Wang CR. Mitochondrial DNA Evidence Supports the Hypothesis that Triodontophorus Species Belong to Cyathostominae. Front Microbiol 2017;8:1444.
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