The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons.
Abstract: Neural tissues from specific pathogen-free ponies that had been experimentally infected with equine herpesvirus 1 (EHV-1) were analysed by in situ hybridization. Digoxigenin-labelled EHV-1 BamHI fragments spanning almost the entire EHV-1 genome were hybridized to RNA in tissue sections from latently infected trigeminal ganglia. The BamHI E fragment detected EHV-1 RNA antisense to gene 63 (HSV-1 homologue ICP0) in a small number of neurons. Sixteen other BamHI fragments gave negative results in 20 sections tested with each fragment. Latency associated transcripts (LATs) were localized to the neuronal nuclei. EHV-1 nucleotide sequence data in the region reveals the presence of a putative EHV-1 LAT promoter that shares a similar motifs with the HSV-1 LAT promoter, including the LAT promoter-binding factor, and may have a role in EHV-1 LAT expression.
Publication Date: 1995-12-01 PubMed ID: 8847517DOI: 10.1099/0022-1317-76-12-3113Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the presence of latency-associated transcripts (LATs) of Equine Herpesvirus 1 (EHV-1) in horse neural tissues, using an experimental technique called in situ hybridization.
Latency-Associated Transcripts Detection
- The researchers first infected ponies with EHV-1, particularly those which are free of specific pathogen. Following this, neural tissues from these ponies were examined.
- The examination was conducted through in situ hybridization, a technique that enables viewing of specific DNA or RNA molecules in tissue sections.
- During this process, fragments across nearly all the EHV-1 genome were marked with Digoxigenin (a steroid used as a molecular probe) and then introduced to RNA in tissue sections. These Digoxigenin-labelled fragments are also known as BamHI fragments.
Findings from the Investigation
- The researchers found that the BamHI E fragment (a specific piece of the EHV-1 genome) detected RNA corresponding to EHV-1 in a minor number of neurons.
- This RNA is ‘antisense’ to the EHV-1 gene 63, meaning it has the opposite sequence to this gene. This gene is a homolog to ICP0, a gene found in a human herpesvirus (HSV-1).
- Negative results were observed from 16 other BamHI fragments in 20 sections tested, indicating that these fragments did not bind to any corresponding RNA in the sample tissues.
- The detected LATs were localized within the nuclei of neurons. LATs are transcripts produced during the latent phase of a herpesvirus infection, when the virus is dormant.
Implications of the Findings
- The study discovered the presence of a potential EHV-1 LAT promoter in the nucleotide sequence. This promoter possesses similar motifs or patterns to the LAT promoter of HSV-1.
- The promoter includes the LAT promoter-binding factor, a protein that indicates it may play a function in the expression or production of EHV-1 LATs.
- Through understanding LATs in EHV-1, it could help understand how herpesviruses establish and maintain latency, potentially influencing therapeutic approaches in managing these viruses.
Cite This Article
APA
Baxi MK, Efstathiou S, Lawrence G, Whalley JM, Slater JD, Field HJ.
(1995).
The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons.
J Gen Virol, 76 ( Pt 12), 3113-3118.
https://doi.org/10.1099/0022-1317-76-12-3113 Publication
Researcher Affiliations
- Centre for Veterinary Science, Cambridge University Veterinary School, UK.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Nucleus / virology
- Herpesvirus 1, Equid / genetics
- Herpesvirus 1, Equid / physiology
- Herpesvirus 1, Human / genetics
- Horses
- In Situ Hybridization
- Molecular Sequence Data
- Promoter Regions, Genetic / genetics
- RNA Probes
- RNA, Antisense / analysis
- RNA, Messenger / analysis
- RNA, Viral / analysis
- Specific Pathogen-Free Organisms
- Trigeminal Ganglion / virology
- Viral Proteins / genetics
- Virus Latency / genetics
- Virus Shedding
Citations
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