FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Veterinary sciences2021; 8(10); 208; doi: 10.3390/vetsci8100208

The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection.

Abstract: Bovine papillomavirus (BPV) types 1 and 2 are recognized as the main cause of equine sarcoids. However, some studies report that up to a quarter of these tumors do not contain detectible BPV1 or BPV2 DNA. The absence of detectible BPV1 or BPV2 in these sarcoids suggests the possible involvement of other papillomavirus types. Currently, five are recognized to cause mesenchymal neoplasia after cross-species infection. In addition to BPV1 and BPV2, BPV13 has been associated with equine sarcoids in Brazil, BPV14 has been associated with feline sarcoids, and Ovis aries papillomavirus 2 caused a sarcoid-like lesion in a pig. To investigate the cause of equine sarcoids, PCR primers were developed to specifically amplify each of the five different that have been associated with mesenchymal neoplasia. The specificity of these primers was confirmed using samples of formalin-fixed tissue known to contain each PV type. These primers allow rapid and sensitive detection of DNA in equine sarcoids. As studies have revealed marked regional variability in the cause of equine sarcoids, these primers will be useful to determine the predominant PV type causing sarcoids in a region. Additionally, there is a single report describing mixed infections by BPV1 and BPV2 in equine sarcoids. The specific primer sets are expected to enable more sensitive detection of mixed infections in equine sarcoids. Determining the cause of equine sarcoids is important as vaccines are developed to prevent these common malignant neoplasms.
Get Full Text
Publication Date: 2021-09-26 PubMed ID: 34679038PubMed Central: PMC8541201DOI: 10.3390/vetsci8100208Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research involves the development of Polymerase Chain Reaction (PCR) primer sets that are designed to be able to differentiate and identify each of the five types of deltapapillomaviruses linked to causing neoplasia (tumor formation). This tool aims to assist in the more precise diagnosis of equine sarcoids, a common type of horse tumor, which can often be caused by varying types of these viruses in different regions or in mixed-infection scenarios.

Objective and Significance

  • The research aims to develop PCR primer sets that are capable of specifically identifying and differentiating each of the five variants of Deltapapillomaviruses (BPV 1, 2, 13, 14 and Ovis aries papillomavirus 2).
  • These viruses are associated with the formation of neoplasia or tumors following cross-species infections, such as equine sarcoids in horses, which are commonly caused by BPV1 and BPV2.
  • A more accurate diagnosis of the cause of these equine sarcoids could aid in the development of more effective vaccines and treatments.

Methodology

  • The newly developed PCR primers were used to attempt to specifically amplify each of the five different Deltapapillomaviruses.
  • Their specificity was confirmed through their application onto samples of formalin-fixed tissue that were identified to contain each type of papillomavirus (PV).

Key Findings

  • The novel PCR primers enabled the rapid and highly sensitive detection of Deltapapillomavirus DNA in equine sarcoids.
  • Since studies have shown that there can be significant regional variability in the specific type of Deltapapillomavirus causing the equine sarcoids, these primers may be crucial in identifying the predominant PV type in a particular region.
  • The primers also have the potential to improve the detection of mixed infections of Deltapapillomaviruses in equine sarcoids, as evidenced by a report describing mixed infections by BPV1 and BPV2 in equine sarcoids.

Implications

  • The findings are significant as they provide a mechanism for better understanding and diagnosing the cause of equine sarcoids, which can be crucial in curbing these common malignant tumors in horses.
  • The development of these specific primers could aid in the future generation of specific treatments or preventive vaccines for equine sarcoids.

Cite This Article

APA
Munday JS, Gedye K, Daudt C, Chaves Da Silva F. (2021). The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection. Vet Sci, 8(10), 208. https://doi.org/10.3390/vetsci8100208

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 8
Issue: 10
PII: 208

Researcher Affiliations

Munday, John S
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.
Gedye, Kristene
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.
Daudt, Cíntia
  • Laboratório de Virologia Geral e Parasitologia, Universidade Federal do Acre, Rio Branco 69920-900, Brazil.
Chaves Da Silva, Flavio
  • Laboratório de Virologia Geral e Parasitologia, Universidade Federal do Acre, Rio Branco 69920-900, Brazil.

Grant Funding

  • ET5.2020 / New Zealand Equine Trust

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 27 references
  1. Rector A, Van Ranst M. Animal papillomaviruses.. Virology 2013 Oct;445(1-2):213-23.
    doi: 10.1016/j.virol.2013.05.007pubmed: 23711385google scholar: lookup
  2. Doorbar J, Quint W, Banks L, Bravo IG, Stoler M, Broker TR, Stanley MA. The biology and life-cycle of human papillomaviruses.. Vaccine 2012 Nov 20;30 Suppl 5:F55-70.
    doi: 10.1016/j.vaccine.2012.06.083pubmed: 23199966google scholar: lookup
  3. Bernard HU, Burk RD, Chen Z, van Doorslaer K, zur Hausen H, de Villiers EM. Classification of papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendments.. Virology 2010 May 25;401(1):70-9.
    doi: 10.1016/j.virol.2010.02.002pmc: PMC3400342pubmed: 20206957google scholar: lookup
  4. Nasir L, Brandt S. Papillomavirus associated diseases of the horse.. Vet Microbiol 2013 Nov 29;167(1-2):159-67.
    doi: 10.1016/j.vetmic.2013.08.003pubmed: 24016387google scholar: lookup
  5. Bogaert L, Van Poucke M, De Baere C, Dewulf J, Peelman L, Ducatelle R, Gasthuys F, Martens A. Bovine papillomavirus load and mRNA expression, cell proliferation and p53 expression in four clinical types of equine sarcoid.. J Gen Virol 2007 Aug;88(Pt 8):2155-2161.
    doi: 10.1099/vir.0.82876-0pubmed: 17622617google scholar: lookup
  6. Carr EA, Théon AP, Madewell BR, Griffey SM, Hitchcock ME. Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States.. Am J Vet Res 2001 May;62(5):741-4.
    doi: 10.2460/ajvr.2001.62.741pubmed: 11341396google scholar: lookup
  7. Bloch N, Breen M, Spradbrow PB. Genomic sequences of bovine papillomaviruses in formalin-fixed sarcoids from Australian horses revealed by polymerase chain reaction.. Vet Microbiol 1994 Jul;41(1-2):163-72.
    doi: 10.1016/0378-1135(94)90145-7pubmed: 7801519google scholar: lookup
  8. Wobeser BK, Davies JL, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL. Epidemiology of equine sarcoids in horses in western Canada.. Can Vet J 2010 Oct;51(10):1103-8.
    pmc: PMC2942047pubmed: 21197201
  9. Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids.. J Clin Microbiol 2013 Jul;51(7):2167-71.
    doi: 10.1128/JCM.00371-13pmc: PMC3697707pubmed: 23637294google scholar: lookup
  10. Munday JS, Knight CG, Howe L. The same papillomavirus is present in feline sarcoids from North America and New Zealand but not in any non-sarcoid feline samples.. J Vet Diagn Invest 2010 Jan;22(1):97-100.
    doi: 10.1177/104063871002200119pubmed: 20093693google scholar: lookup
  11. Munday JS, Thomson N, Dunowska M, Knight CG, Laurie RE, Hills S. Genomic characterisation of the feline sarcoid-associated papillomavirus and proposed classification as Bos taurus papillomavirus type 14.. Vet Microbiol 2015 Jun 12;177(3-4):289-95.
    doi: 10.1016/j.vetmic.2015.03.019pubmed: 25840470google scholar: lookup
  12. Munday JS, Fairley R, Lowery I. Detection of Ovis aries papillomavirus type 2 DNA sequences in a sarcoid-like mass in the mouth of a pig.. Vet Microbiol 2020 Sep;248:108801.
    doi: 10.1016/j.vetmic.2020.108801pubmed: 32827929google scholar: lookup
  13. Hainisch EK, Abel-Reichwald H, Shafti-Keramat S, Pratscher B, Corteggio A, Borzacchiello G, Wetzig M, Jindra C, Tichy A, Kirnbauer R, Brandt S. Potential of a BPV1 L1 VLP vaccine to prevent BPV1- or BPV2-induced pseudo-sarcoid formation and safety and immunogenicity of EcPV2 L1 VLPs in horse.. J Gen Virol 2017 Feb;98(2):230-241.
    doi: 10.1099/jgv.0.000673pubmed: 28284277google scholar: lookup
  14. Hainisch EK, Brandt S, Shafti-Keramat S, Van den Hoven R, Kirnbauer R. Safety and immunogenicity of BPV-1 L1 virus-like particles in a dose-escalation vaccination trial in horses.. Equine Vet J 2012 Jan;44(1):107-11.
    doi: 10.1111/j.2042-3306.2011.00390.xpmc: PMC3939654pubmed: 21895749google scholar: lookup
  15. Malagón T, Drolet M, Boily MC, Franco EL, Jit M, Brisson J, Brisson M. Cross-protective efficacy of two human papillomavirus vaccines: a systematic review and meta-analysis.. Lancet Infect Dis 2012 Oct;12(10):781-9.
    doi: 10.1016/S1473-3099(12)70187-1pubmed: 22920953google scholar: lookup
  16. Pitisuttithum P, Velicer C, Luxembourg A. 9-Valent HPV vaccine for cancers, pre-cancers and genital warts related to HPV.. Expert Rev Vaccines 2015;14(11):1405-19.
    doi: 10.1586/14760584.2015.1089174pubmed: 26366475google scholar: lookup
  17. Munday JS, Cullum AA, Thomson NA, Bestbier M, McCormack T, Julian AF. Anal fibropapillomas containing bovine papillomavirus type 2 DNA in two groups of heifers.. N Z Vet J 2018 Sep;66(5):267-271.
    doi: 10.1080/00480169.2018.1479317pubmed: 29791810google scholar: lookup
  18. Munday JS, Kiupel M, French AF, Howe L, Squires RA. Detection of papillomaviral sequences in feline Bowenoid in situ carcinoma using consensus primers.. Vet Dermatol 2007 Aug;18(4):241-5.
    doi: 10.1111/j.1365-3164.2007.00600.xpubmed: 17610489google scholar: lookup
  19. Szczerba-Turek A, Siemionek J, Ras A, Bancerz-Kisiel A, Platt-Samoraj A, Lipczynska-Ilczuk K, Szweda W. Genetic evaluation of bovine papillomavirus types detected in equine sarcoids in Poland.. Pol J Vet Sci 2019 Mar;22(1):25-29.
    pubmed: 30997762doi: 10.24425/pjvs.2018.125602google scholar: lookup
  20. Meng Q, Ning C, Wang L, Ren Y, Li J, Xiao C, Li Y, Li Z, He Z, Cai X, Qiao J. Molecular detection and genetic diversity of bovine papillomavirus in dairy cows in Xinjiang, China.. J Vet Sci 2021 Jul;22(4):e50.
    doi: 10.4142/jvs.2021.22.e50pmc: PMC8318792pubmed: 34170091google scholar: lookup
  21. Figueirêdo RP, Santos GF, Oliveira LB, Santos LABO, Barreto DM, Cândido AL, Campos AC, Azevedo EO, Batista MVA. High Genotypic Diversity, Putative New Types and Intra-Genotype Variants of Bovine Papillomavirus in Northeast Brazil.. Pathogens 2020 Sep 15;9(9).
    doi: 10.3390/pathogens9090748pmc: PMC7558504pubmed: 32942526google scholar: lookup
  22. Roperto S, Russo V, Leonardi L, Martano M, Corrado F, Riccardi MG, Roperto F. Bovine Papillomavirus Type 13 Expression in the Urothelial Bladder Tumours of Cattle.. Transbound Emerg Dis 2016 Dec;63(6):628-634.
    doi: 10.1111/tbed.12322pubmed: 25597262google scholar: lookup
  23. Chambers G, Ellsmore VA, O'Brien PM, Reid SWJ, Love S, Campo MS, Nasir L. Association of bovine papillomavirus with the equine sarcoid.. J Gen Virol 2003 May;84(Pt 5):1055-1062.
    doi: 10.1099/vir.0.18947-0pubmed: 12692268google scholar: lookup
  24. Yamashita-Kawanishi N, Chambers JK, Uchida K, Tobari Y, Yoshimura H, Yamamoto M, Yumoto N, Aoki H, Sugiura K, Higuchi T, Saito S, Haga T. Genomic characterisation of bovine papillomavirus types 1 and 2 identified in equine sarcoids in Japan.. Equine Vet J 2021 Nov;53(6):1199-1209.
    doi: 10.1111/evj.13398pubmed: 33300145google scholar: lookup
  25. Manos MM, Ting Shin Y, Wright DK, Lewis AL, Broker TR, Wolinsky SM, Manos M, Ting YC. The use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cells 1989;7:209–214.
  26. Antonsson A, Forslund O, Ekberg H, Sterner G, Hansson BG. The ubiquity and impressive genomic diversity of human skin papillomaviruses suggest a commensalic nature of these viruses.. J Virol 2000 Dec;74(24):11636-41.
    doi: 10.1128/JVI.74.24.11636-11641.2000pmc: PMC112445pubmed: 11090162google scholar: lookup
  27. Bialek R, Cirera AC, Herrmann T, Aepinus C, Shearn-Bochsler VI, Legendre AM. Nested PCR assays for detection of Blastomyces dermatitidis DNA in paraffin-embedded canine tissue.. J Clin Microbiol 2003 Jan;41(1):205-8.
    doi: 10.1128/JCM.41.1.205-208.2003pmc: PMC149559pubmed: 12517849google scholar: lookup

Citations

This article has been cited 3 times.
  1. Rolph KE, Cavanaugh RP. Infectious Causes of Neoplasia in the Domestic Cat. Vet Sci 2022 Aug 30;9(9).
    doi: 10.3390/vetsci9090467pubmed: 36136683google scholar: lookup
  2. Emin K, Nuvit C, Serpil D, Enver B, Ataseven VS, Volkan Y, Fırat D, Hilmi N, Celal Sahin E, Ugur A, Mushap K, Ayfer Y. Molecular detection of Papillomavirus and immunohistochemical investigation of p53 gene expressions in bovine papillomas and fibropapillomas. Arch Microbiol 2022 Apr 24;204(5):278.
    doi: 10.1007/s00203-022-02902-0pubmed: 35461436google scholar: lookup
  3. Munday JS, Orbell G, Fairley RA, Hardcastle M, Vaatstra B. Evidence from a Series of 104 Equine Sarcoids Suggests That Most Sarcoids in New Zealand Are Caused by Bovine Papillomavirus Type 2, although Both BPV1 and BPV2 DNA Are Detectable in around 10% of Sarcoids. Animals (Basel) 2021 Oct 29;11(11).
    doi: 10.3390/ani11113093pubmed: 34827825google scholar: lookup
Subscribe to our newsletter
Join 99,850 horse owners like you who receive our email updates on horse health and nutrition.