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The Journal of comparative neurology1984; 227(1); 78-91; doi: 10.1002/cne.902270109

The distribution and origin of a novel brain peptide, neuropeptide Y, in the spinal cord of several mammals.

Abstract: The distribution of neuropeptide Y [NPY]-immunoreactive material was examined in the spinal cord and dorsal root ganglia of rat, guinea-pig, cat, marmoset, and horse. Considerable concentrations of NPY and similar distribution patterns of immunoreactive nerve fibres were found in the spinal cord of all species investigated. The dorsal root ganglia of the cat and the horse contained numerous immunoreactive nerve fibres, but in these species, as in the other three studied [rat, guinea-pig, marmoset], no positively stained cell bodies were found. Neuropeptide Y-immunoreactive nerves were observed at all levels of the spinal cord, being most concentrated in the dorsal horn. In the rat, guinea-pig, and marmoset, there was a marked increase of NPY-immunoreactive fibres in the lumbosacral regions of the spinal cord, and this was reflected by a considerable increase of extractable NPY. Estimations of NPY-immunoreactive material in the various regions of the rat spinal cord were as follows: cervical, 13.8 +/- 1.0; thoracic, 21.1 +/- 2.5; lumbar, 16.3 +/- 2.9; sacral, 92.4 +/- 8.5 pmol/gm wet weight of tissue +/- SEM. In the ventral portion of the guinea-pig spinal cord they were as follows: cervical, 7.1 +/- 1.2; thoracic, 8.2 +/- 3.6; lumbar, 22.6 +/- 7.0; sacral, 36.7 +/- 9.5 pmol/gm wet weight of tissue +/- SEM. Analysis of spinal cord extracts by reverse phase high performance liquid chromatography [HPLC] demonstrated that NPY-immunoreactive material elutes in the position of pure NPY standard. No changes in the concentration and distribution of the NPY-like material in the rat spinal cord were observed following a variety of surgical and pharmacological manipulations, including cervical rhizotomy, sciatic nerve section and ligation, and local application of capsaicin [50 mM] to one sciatic nerve. It is therefore suggested that most of the NPY-immunoreactive material in the spinal cord is derived either from intrinsic nerve cell bodies or from supraspinal tracts.
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Publication Date: 1984-07-20 PubMed ID: 6432858DOI: 10.1002/cne.902270109Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on studying the distribution and origin of the brain peptide called neuropeptide Y (NPY) in the spinal cord of multiple mammalian species.

Objective and Methodology

  • The objective of the research was to study the presence and distribution of neuropeptide Y (NPY) in the spinal cord and dorsal root ganglia of various mammals including rat, guinea-pig, cat, marmoset, and horse.
  • The methodology involved analyzing the concentrations of NPY and observing the distribution patterns of nerve fibers in the spinal cord of each species. The patterns were visualized using a specific method that highlights immunoreactive nerve fibers, which react specifically to the application of antibodies against NPY.

Results

  • The study found substantial concentrations of NPY in the spinal cords of all investigated species and similar distribution patterns of immunoreactive nerve fibers.
  • NPY-immunoreactive nerves were observed across all levels of the spinal cord, with a major concentration in the dorsal horn of rat, guinea-pig, and marmoset, and similarly in the lumbosacral regions of their spinal cord. The researchers quantified these observations in the rat and guinea-pig spinal cord regions using weight-related measurements.
  • The dorsal root ganglia of the cat and the horse were found to contain many immunoreactive nerve fibers. However, the study found no positively stained cell bodies in these species or in the others.
  • The researchers used high performance liquid chromatography (HPLC) to analyze the spinal cord extracts, confirming that the NPY-immunoreactive material indeed corresponds to NPY.

Conclusions

  • The presence and distribution of NPY-immunoreactive material in the spinal cord did not change after a variety of surgical and pharmacological manipulations in the rat. This lead to the suggestion that the majority of the NPY-immunoreactive material in the spinal cord originates either from intrinsic nerve cell bodies or from supraspinal tracts, not from peripheral sensory nerve fibers as commonly believed.
  • This finding theorizes a potential significant role of this neuropeptide within the spinal cord that could lead to further advances in understanding neurological functions and potential implications in neurodegenerative disorders.

Cite This Article

APA
Gibson SJ, Polak JM, Allen JM, Adrian TE, Kelly JS, Bloom SR. (1984). The distribution and origin of a novel brain peptide, neuropeptide Y, in the spinal cord of several mammals. J Comp Neurol, 227(1), 78-91. https://doi.org/10.1002/cne.902270109

Publication

The Journal of comparative neurology
ISSN: 0021-9967
NlmUniqueID: 0406041
Country: United States
Language: English
Volume: 227
Issue: 1
Pages: 78-91

Researcher Affiliations

Gibson, S J
    Polak, J M
      Allen, J M
        Adrian, T E
          Kelly, J S
            Bloom, S R

              MeSH Terms

              • Animals
              • Callitrichinae
              • Chromatography, High Pressure Liquid
              • Ganglia, Spinal / analysis
              • Ganglia, Spinal / metabolism
              • Guinea Pigs
              • Horses
              • Nerve Tissue Proteins / analysis
              • Nerve Tissue Proteins / metabolism
              • Neuropeptide Y
              • Radioimmunoassay
              • Rats
              • Species Specificity
              • Spinal Cord / analysis
              • Spinal Cord / metabolism

              Citations

              This article has been cited 31 times.
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