The distribution of types I and III collagen and fibronectin in the healing equine tendon.
Abstract: During tissue response to injury the glycoproteins fibronectin and Type III collagen are synthesized in increased amounts. We have studied the distribution of these molecules in the healing tendon at various times after injury by comparison with that of the major constituent of normal tendon, Type I collagen. Immunofluorescent localization demonstrated the presence of fibronectin throughout the tendon within one week after injury. Staining was found in the matrix, both around capillaries and around fibroblast-like cells. Fibronectin was still apparent in the healing tendon at one month after injury, but after a further two months was no longer detectable. Type III collagen was present both in pericellular and matrix locations until three months after injury, and matrix staining was apparent during the entire fourteen-month period under study. Type III collagen was also found throughout the matrix of the contralateral superficial flexor tendon during this period.
Publication Date: 1984-01-01 PubMed ID: 6478822DOI: 10.3109/03008208409013684Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article focuses on the study of the distribution patterns of the proteins fibronectin and Type III collagen in injured equine tendons, and its comparison with the pattern of Type I collagen, a key component of healthy tendons.
Objective of the Research
- The primary objective of this research was to understand the distribution and presence of Type I and Type III collagen, and fibronectin glycoproteins in injured equine tendons. The intent was to compare their quantities and locations over the healing period following an injury.
Methodology
- Immunofluorescent localization was used as the methodology to visualize and study the distribution of these proteins in tendon tissue.
- Observations were made on the healing tendons at different stages – one week, one month, two months, three months, and a longer period of fourteen months after the injury.
Key Findings
- Fibronectin was found to be present in the tendon tissue within only a week of injury. It was found distributed in the matrix, around fibroblast-like cells and capillaries.
- However, fibronectin only remained detectable in the healing tendon up to one month following injury. After another two months, it was no longer detectable.
- On the other hand, Type III collagen was present both around the fibroblast cells and in the larger matrix of the tendon until three months post-injury. It was still evident in the tendon matrix till the end of the fourteen-month period studied.
- Interestingly, Type III collagen was also noticed distributed throughout the matrix of the unaffected, contralateral superficial flexor tendon during the study’s duration.
Significance of the Findings
- The patterns of protein distribution indicated in this research can be significant in understanding the healing process of tendons following injury.
- The presence of Type III collagen for a more prolonged period than fibronectin suggests its significant role in the healing and remodeling of tendon tissue following injury.
- The detection of Type III collagen in the contralateral healthy tendon indicates a possible systemic or generalized response to injury.
Cite This Article
APA
Williams IF, McCullagh KG, Silver IA.
(1984).
The distribution of types I and III collagen and fibronectin in the healing equine tendon.
Connect Tissue Res, 12(3-4), 211-227.
https://doi.org/10.3109/03008208409013684 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Collagen / analysis
- Fibronectins / analysis
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Tendon Injuries / metabolism
- Tendon Injuries / pathology
- Tendon Injuries / veterinary
- Wound Healing
Citations
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