The effect of benzo(a)pyrene on fertility, primordial oocyte number, and ovarian response to pregnant mare’s serum gonadotropin.
Abstract: The polycyclic aromatic hydrocarbon (PAH) benzo(a)pyrene (BP) reduced the fertility of DBA/2N mice in a dose-dependent fashion. Control mice produced offspring at a rate of 0.91 pups/mouse per week. Treatment with BP at doses of 10, 100, 200, and 500 mg/kg decreased offspring production rates to 0.61, 0.20, zero and zero pups/mouse per week, respectively. BP also destroyed primordial oocytes in similarly treated mice. Treatment with BP at doses of 10, 50, 100, and 500 mg/kg destroyed 20%, 58%, 88%, and 100%, respectively, of the primordial oocytes in DBA/2N mouse ovaries. Dose-response curves for fertility reduction and primordial oocyte destruction were identical. The threshold for fertility reduction was 3.4 mg/kg and for primordial oocyte destruction it was 2.7 mg/kg. The 50% effect doses for fertility reduction and primordial oocyte destruction were 25.5 mg/kg and 24.5 mg/kg, respectively. Although BP reduced fertility and destroyed primordial oocytes, it had no effect on the ovarian weight response to pregnant mare's serum gonadotropin (PMSG), consistent with the observations that BP at these doses does not destroy growing or preovulatory oocytes or follicles. The similarity of the dose-response curves for fertility reduction and primordial oocyte destruction suggested that their mechanism of action is similar and resides within the ovary. As BP had no effect on ovarian response to PMSG, the effect was likely to reside outside the regulatory mechanisms controlling ovulation. These data suggest that the site of fertility reduction by BP may reside in the mechanisms of fertilization, implantation, or early conceptus development.
Publication Date: 1980-01-01 PubMed ID: 6287394
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- Journal Article
Summary
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The research study demonstrates the effect of benzo(a)pyrene (BP), a polycyclic aromatic hydrocarbon, on the fertility of DBA/2N mice, including its damaging effect on oocytes and its lack of effect on ovarian weight in response to a specific gonadotropin. The research reveals how BP reduces fertility and the destruction it causes on a cellular level, suggesting its impact on processes such as fertilization, implantation, or early development stages.
Objectives of the Study
- The primary aim of the study was to assess the impact of the polycyclic aromatic hydrocarbon, benzo(a)pyrene (BP), on the fertility rates in DBA/2N mice.
- The study also investigated the effect of BP on the amount of primordial oocytes in similarly treated mice.
- Additionally, the research sought to establish any correlation between BP dosage and a decrease in fertility, while determining the threshold levels for fertility reduction and oocyte destruction.
Key Findings
- The fertility rates in the mice decreased in a dose-dependent manner as the dosage of BP increased. Similarly, the destruction of primordial oocytes also increased with higher BP dosage.
- Dose-response curves for fertility reduction and primordial oocyte destruction were identical, suggesting a similar mechanism of action within the ovary.
- Despite causing fertility reduction and oocyte destruction, BP did not affect ovarian weight response to pregnant mare’s serum gonadotropin, implying its effect likely resides outside the regulatory mechanisms controlling ovulation.
Implications
- The findings suggest that the reduction in fertility caused by BP might be linked to processes such as fertilization, implantation, or early conceptus development. This discovery has critical implications for a better understanding of how external compounds, such as BP, can affect fertility levels.
Limitations and Future Research
- The study was conducted on DBA/2N mice, and the results may not directly apply to other species. Future research could broaden the scope to include other animals for a more holistic view.
- Despite the damaging effects of BP on fertility and oocytes, more research is needed to fully understand the specific mechanisms of action, including its associated factors and underlying processes that lead to these impacts.
Cite This Article
APA
Mattison DR, White NB, Nightingale MR.
(1980).
The effect of benzo(a)pyrene on fertility, primordial oocyte number, and ovarian response to pregnant mare’s serum gonadotropin.
Pediatr Pharmacol (New York), 1(2), 143-151.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Benzo(a)pyrene
- Benzopyrenes / pharmacology
- Dose-Response Relationship, Drug
- Female
- Fertility / drug effects
- Gonadotropins, Equine / pharmacology
- Male
- Mice
- Mice, Inbred DBA
- Oocytes / drug effects
- Ovary / drug effects
- Ovum / drug effects
- Time Factors
Citations
This article has been cited 10 times.- Rafiee A, Hoseini M, Akbari S, Mahabee-Gittens EM. Exposure to Polycyclic Aromatic Hydrocarbons and adverse reproductive outcomes in women: current status and future perspectives.. Rev Environ Health 2023 Jan 2;.
- Luderer U, Lim J, Ortiz L, Nguyen JD, Shin JH, Allen BD, Liao LS, Malott K, Perraud V, Wingen LM, Arechavala RJ, Bliss B, Herman DA, Kleinman MT. Exposure to environmentally relevant concentrations of ambient fine particulate matter (PM(2.5)) depletes the ovarian follicle reserve and causes sex-dependent cardiovascular changes in apolipoprotein E null mice.. Part Fibre Toxicol 2022 Jan 7;19(1):5.
- Cotena M, Auffan M, Tassistro V, Resseguier N, Rose J, Perrin J. In Vitro Co-Exposure to CeO(2) Nanomaterials from Diesel Engine Exhaust and Benzo(a)Pyrene Induces Additive DNA Damage in Sperm and Cumulus Cells but Not in Oocytes.. Nanomaterials (Basel) 2021 Feb 13;11(2).
- Luderer U, Meier MJ, Lawson GW, Beal MA, Yauk CL, Marchetti F. In Utero Exposure to Benzo[a]pyrene Induces Ovarian Mutations at Doses That Deplete Ovarian Follicles in Mice.. Environ Mol Mutagen 2019 Jun;60(5):410-420.
- Luderer U, Myers MB, Banda M, McKim KL, Ortiz L, Parsons BL. Ovarian effects of prenatal exposure to benzo[a]pyrene: Roles of embryonic and maternal glutathione status.. Reprod Toxicol 2017 Apr;69:187-195.
- Luderer U, Christensen F, Johnson WO, She J, Ip HS, Zhou J, Alvaran J, Krieg EF Jr, Kesner JS. Associations between urinary biomarkers of polycyclic aromatic hydrocarbon exposure and reproductive function during menstrual cycles in women.. Environ Int 2017 Mar;100:110-120.
- Lim J, Ortiz L, Nakamura BN, Hoang YD, Banuelos J, Flores VN, Chan JY, Luderer U. Effects of deletion of the transcription factor Nrf2 and benzo [a]pyrene treatment on ovarian follicles and ovarian surface epithelial cells in mice.. Reprod Toxicol 2015 Dec;58:24-32.
- Whitehead A. Interactions between oil-spill pollutants and natural stressors can compound ecotoxicological effects.. Integr Comp Biol 2013 Oct;53(4):635-47.
- Archibong AE, Ramesh A, Inyang F, Niaz MS, Hood DB, Kopsombut P. Endocrine disruptive actions of inhaled benzo(a)pyrene on ovarian function and fetal survival in fisher F-344 adult rats.. Reprod Toxicol 2012 Dec;34(4):635-43.
- Hoang YD, Nakamura BN, Luderer U. Follicle-stimulating hormone and estradiol interact to stimulate glutathione synthesis in rat ovarian follicles and granulosa cells.. Biol Reprod 2009 Oct;81(4):636-46.
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