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Pediatric pharmacology (New York, N.Y.)1980; 1(2); 143-151;

The effect of benzo(a)pyrene on fertility, primordial oocyte number, and ovarian response to pregnant mare’s serum gonadotropin.

Abstract: The polycyclic aromatic hydrocarbon (PAH) benzo(a)pyrene (BP) reduced the fertility of DBA/2N mice in a dose-dependent fashion. Control mice produced offspring at a rate of 0.91 pups/mouse per week. Treatment with BP at doses of 10, 100, 200, and 500 mg/kg decreased offspring production rates to 0.61, 0.20, zero and zero pups/mouse per week, respectively. BP also destroyed primordial oocytes in similarly treated mice. Treatment with BP at doses of 10, 50, 100, and 500 mg/kg destroyed 20%, 58%, 88%, and 100%, respectively, of the primordial oocytes in DBA/2N mouse ovaries. Dose-response curves for fertility reduction and primordial oocyte destruction were identical. The threshold for fertility reduction was 3.4 mg/kg and for primordial oocyte destruction it was 2.7 mg/kg. The 50% effect doses for fertility reduction and primordial oocyte destruction were 25.5 mg/kg and 24.5 mg/kg, respectively. Although BP reduced fertility and destroyed primordial oocytes, it had no effect on the ovarian weight response to pregnant mare's serum gonadotropin (PMSG), consistent with the observations that BP at these doses does not destroy growing or preovulatory oocytes or follicles. The similarity of the dose-response curves for fertility reduction and primordial oocyte destruction suggested that their mechanism of action is similar and resides within the ovary. As BP had no effect on ovarian response to PMSG, the effect was likely to reside outside the regulatory mechanisms controlling ovulation. These data suggest that the site of fertility reduction by BP may reside in the mechanisms of fertilization, implantation, or early conceptus development.
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Publication Date: 1980-01-01 PubMed ID: 6287394
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study demonstrates the effect of benzo(a)pyrene (BP), a polycyclic aromatic hydrocarbon, on the fertility of DBA/2N mice, including its damaging effect on oocytes and its lack of effect on ovarian weight in response to a specific gonadotropin. The research reveals how BP reduces fertility and the destruction it causes on a cellular level, suggesting its impact on processes such as fertilization, implantation, or early development stages.

Objectives of the Study

  • The primary aim of the study was to assess the impact of the polycyclic aromatic hydrocarbon, benzo(a)pyrene (BP), on the fertility rates in DBA/2N mice.
  • The study also investigated the effect of BP on the amount of primordial oocytes in similarly treated mice.
  • Additionally, the research sought to establish any correlation between BP dosage and a decrease in fertility, while determining the threshold levels for fertility reduction and oocyte destruction.

Key Findings

  • The fertility rates in the mice decreased in a dose-dependent manner as the dosage of BP increased. Similarly, the destruction of primordial oocytes also increased with higher BP dosage.
  • Dose-response curves for fertility reduction and primordial oocyte destruction were identical, suggesting a similar mechanism of action within the ovary.
  • Despite causing fertility reduction and oocyte destruction, BP did not affect ovarian weight response to pregnant mare’s serum gonadotropin, implying its effect likely resides outside the regulatory mechanisms controlling ovulation.

Implications

  • The findings suggest that the reduction in fertility caused by BP might be linked to processes such as fertilization, implantation, or early conceptus development. This discovery has critical implications for a better understanding of how external compounds, such as BP, can affect fertility levels.

Limitations and Future Research

  • The study was conducted on DBA/2N mice, and the results may not directly apply to other species. Future research could broaden the scope to include other animals for a more holistic view.
  • Despite the damaging effects of BP on fertility and oocytes, more research is needed to fully understand the specific mechanisms of action, including its associated factors and underlying processes that lead to these impacts.

Cite This Article

APA
Mattison DR, White NB, Nightingale MR. (1980). The effect of benzo(a)pyrene on fertility, primordial oocyte number, and ovarian response to pregnant mare’s serum gonadotropin. Pediatr Pharmacol (New York), 1(2), 143-151.

Publication

Pediatric pharmacology (New York, N.Y.)
ISSN: 0270-322X
NlmUniqueID: 8400778
Country: United States
Language: English
Volume: 1
Issue: 2
Pages: 143-151

Researcher Affiliations

Mattison, D R
    White, N B
      Nightingale, M R

        MeSH Terms

        • Animals
        • Benzo(a)pyrene
        • Benzopyrenes / pharmacology
        • Dose-Response Relationship, Drug
        • Female
        • Fertility / drug effects
        • Gonadotropins, Equine / pharmacology
        • Male
        • Mice
        • Mice, Inbred DBA
        • Oocytes / drug effects
        • Ovary / drug effects
        • Ovum / drug effects
        • Time Factors

        Citations

        This article has been cited 11 times.
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        6. Luderer U, Christensen F, Johnson WO, She J, Ip HS, Zhou J, Alvaran J, Krieg EF Jr, Kesner JS. Associations between urinary biomarkers of polycyclic aromatic hydrocarbon exposure and reproductive function during menstrual cycles in women. Environ Int 2017 Mar;100:110-120.
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        7. Lim J, Ortiz L, Nakamura BN, Hoang YD, Banuelos J, Flores VN, Chan JY, Luderer U. Effects of deletion of the transcription factor Nrf2 and benzo [a]pyrene treatment on ovarian follicles and ovarian surface epithelial cells in mice. Reprod Toxicol 2015 Dec;58:24-32.
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          doi: 10.1093/icb/ict080pubmed: 23842611google scholar: lookup
        9. Archibong AE, Ramesh A, Inyang F, Niaz MS, Hood DB, Kopsombut P. Endocrine disruptive actions of inhaled benzo(a)pyrene on ovarian function and fetal survival in fisher F-344 adult rats. Reprod Toxicol 2012 Dec;34(4):635-43.
          doi: 10.1016/j.reprotox.2012.09.003pubmed: 23059060google scholar: lookup
        10. Hoang YD, Nakamura BN, Luderer U. Follicle-stimulating hormone and estradiol interact to stimulate glutathione synthesis in rat ovarian follicles and granulosa cells. Biol Reprod 2009 Oct;81(4):636-46.
          doi: 10.1095/biolreprod.109.078378pubmed: 19516019google scholar: lookup
        11. Novbatova G, Fox I, Timme K, Keating AF. High fat diet-induced obesity and gestational DMBA exposure alter folliculogenesis and the proteome of the maternal ovary†. Biol Reprod 2024 Aug 15;111(2):496-511.
          doi: 10.1093/biolre/ioae070pubmed: 38813940google scholar: lookup
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