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Home / Equine Research Bank / Equine Vet J / The effect of continuous digital hypothermia on lamellar ene...
Equine veterinary journal2020; 52(4); 585-592; doi: 10.1111/evj.13215

The effect of continuous digital hypothermia on lamellar energy metabolism and perfusion during laminitis development in two experimental models.

Abstract: Continuous digital hypothermia (CDH) prevents lamellar failure in the euglycaemic hyperinsulinaemic clamp (EHC) and oligofructose (OF) laminitis models, but the mechanisms remain unclear. Objective: To evaluate the effects of CDH on lamellar energy metabolism and perfusion in healthy horses and during EHC and OF laminitis models. Methods: In vivo experiment. Methods: Archived samples were used from Standardbred geldings that received no treatment (CON) (n = 8) or underwent EHC (n = 8) or OF (n = 6) laminitis models. Both forelimbs were instrumented with a lamellar microdialysis system, and one forelimb was cooled (CDH) with the other maintained at ambient temperature (AMB). Microdialysate was collected every 6 hours and analysed for glucose, lactate and pyruvate concentrations and lactate to pyruvate ratio (L:P). Microdialysis urea clearance was used to estimate lamellar tissue perfusion. Data were analysed using a mixed-effects linear regression model. Results: Glucose did not change in CDH limbs relative to AMB in CON (P = .3), EHC (P = .3) or OF (P = .6) groups. There was a decrease in lactate (P < .001) and pyruvate (P < .01) in CDH limbs relative to AMB in all groups. L:P decreased in CON CDH relative to CON AMB (P < .001) but was not different in EHC (P = .6) and OF (P = .07) groups. Urea clearance decreased in CDH limbs relative to AMB in CON (P = .002) and EHC (P < .001), but not in OF (P = .4). Conclusions: The EHC model may not mimic natural endocrinopathic laminitis. Conclusions: CDH caused a marked decrease in lamellar glucose metabolism (CON, EHC and OF) and perfusion (CON and EHC) without affecting lamellar glucose concentration. Although cellular energy failure is not a primary pathophysiological event in EHC and OF laminitis models, CDH may act by limiting energy supply to pathologic cellular processes whilst preserving those critical to lamellar homoeostasis.
© 2019 EVJ Ltd.
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Publication Date: 2020-02-12 PubMed ID: 31793047DOI: 10.1111/evj.13215Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines how continuous digital hypothermia (CDH) affects the metabolism and blood flow in the lamellae (a part of the horse’s hoof) during the development of laminitis, a condition that can induce foot pain in horses. It looks at the effects of CDH using two different experimental models for laminitis.

Methods

  • The research involved experimentations in vivo, meaning on live specimens.
  • Samples were taken from Standardbred geldings which received no treatment (CON, control group), or underwent euglycaemic hyperinsulinaemic clamp (EHC) or oligofructose (OF) laminitis models.
  • The lamellar microdialysis system was used in both forelimbs of the test subjects. One forelimb was cooled (CDH) while the other was kept at ambient temperature (AMB).
  • Microdialysate was collected every 6 hours and analysed for glucose, lactate and pyruvate concentrations, and the lactate to pyruvate ratio (L:P).

Results

  • Glucose levels did not change in the cooled limbs when compared to those kept at ambient temperature in all three groups (CON, EHC, OF).
  • The levels of lactate and pyruvate decreased in the cooled limbs relative to the control in all groups.
  • There was a decrease in the L:P ratio in the control group with cooled limbs compared to the non-cooled ones, but these results were not seen in the EHC and OF groups.
  • Urea clearance, which was used to estimate the blood flow within lamellar tissue, decreased in the cooled limbs relative to those kept at ambient temperature in the control and EHC groups, but not in the OF group.

Conclusions

  • The results suggest that the EHC model may not mimic natural laminitis.
  • Continuous digital hypothermia caused a significant decrease in glucose metabolism and blood flow in the lamellae without altering glucose concentration. This was observed across all groups (CON, EHC, and OF).
  • Despite the results suggesting that energy failure at the cellular level is not a primary issue in EHC and OF laminitis models, it’s hypothesized that CDH may work by limiting energy supply to harmful cellular processes, while preserving those necessary for maintaining normal lamellar function.

Cite This Article

APA
Stokes SM, Bertin FR, Stefanovski D, Poulsen L, Belknap JK, Medina-Torres CE, Pollitt CC, van Eps AW. (2020). The effect of continuous digital hypothermia on lamellar energy metabolism and perfusion during laminitis development in two experimental models. Equine Vet J, 52(4), 585-592. https://doi.org/10.1111/evj.13215

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 52
Issue: 4
Pages: 585-592

Researcher Affiliations

Stokes, Simon M
  • Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Bertin, Francois-Rene
  • Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Stefanovski, Darko
  • New Bolton Center, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
Poulsen, Lea
  • Institute of Veterinary Science, University of Liverpool, Liverpool, UK.
Belknap, James K
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
Medina-Torres, Carlos E
  • Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Pollitt, Christopher C
  • Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
van Eps, Andrew W
  • Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
  • New Bolton Center, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.

MeSH Terms

  • Animals
  • Energy Metabolism
  • Foot Diseases / veterinary
  • Hoof and Claw
  • Horse Diseases
  • Horses
  • Hypothermia / veterinary
  • Inflammation / veterinary
  • Male
  • Models, Theoretical

Grant Funding

  • Grayson-Jockey Club Research Foundation

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Citations

This article has been cited 1 times.
  1. Stokes SM, Burns TA, Watts MR, Bertin FR, Stefanovski D, Medina-Torres CE, Belknap JK, van Eps AW. Effect of digital hypothermia on lamellar inflammatory signaling in the euglycemic hyperinsulinemic clamp laminitis model. J Vet Intern Med 2020 Jul;34(4):1606-1613.
    doi: 10.1111/jvim.15835pubmed: 32583504google scholar: lookup
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