The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony.
Abstract: Serum haptoglobin concentration was used as an indicator of the acute phase response in ponies undergoing exploratory laparotomy. Preoperative, 1 h intraoperative, 3 h, 6 h, 12 h and 24 h postoperative blood samples and 48 h postoperative peritoneal fluid samples were obtained for haptoglobin analysis. A spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC) was used to determine haptoglobin concentrations. The preoperative reference range for serum haptoglobin concentrations in these ponies was 25-60 mg CyanBC/dL. Intraoperative and 3 h postoperative blood samples had decreased haptoglobin concentrations when compared to preoperative values. Serum haptoglobin concentrations began to rise by the 6 h postoperative sample and were generally elevated above preoperative values by the 24 h postoperative sample. Two of the ten ponies had mild signs of postoperative colic which were associated with twofold elevations in serum haptoglobin concentrations and fivefold elevations in peritoneal fluid haptoglobin concentrations.
Publication Date: 1993-01-01 PubMed ID: 8431803PubMed Central: PMC1263587
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study observes how the concentration of serum haptoglobin, a marker of inflammation, changes after ponies undergo exploratory laparotomy surgery. The researchers found that the levels of haptoglobin initially decreased, then significantly increased within 24 hours following the operation, with ponies experiencing postoperative complications showing further increased levels in blood and peritoneal fluid.
Research methodology
- The study focuses on using serum haptoglobin concentration in ponies as an indicator of the acute phase response. This response typically includes inflammation and happens shortly after tissue damage or infection.
- The researchers collected samples before the surgery, 1 hour into the surgery, and 3, 6, 12, and 24 hours after the surgery. They also collected fluid from the peritoneum, the cavity lining the abdomen, 48 hours after the operation.
- These samples were analyzed using a spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC), a method that can accurately determine haptoglobin concentrations.
Key findings
- The normal or preoperative range for serum haptoglobin concentrations in the study’s ponies was determined to be between 25-60 mg CyanBC/dL.
- Clear changes were tracked as haptoglobin concentrations decreased during and 3 hours after the operation, compared to pre-surgery levels. Haptoglobin levels then started to rise by 6 hours post-operation and were consistently higher than preoperative levels by the 24th hour post-operation.
- In some cases where postoperative colic (abdominal pain) was observed, there was a marked elevation in haptoglobin concentrations in both serum and peritoneal fluid. Specifically, serum haptoglobin levels rose twofold, and peritoneal fluid haptoglobin levels rose fivefold, signifying a potential correlation between increased haptoglobin levels and associated postoperative complications.
Conclusion
- This research helps to understand the pattern of haptoglobin levels change following abdominal surgery in ponies, akin to exploratory laparotomy. The findings may be useful in detecting postoperative complications early by monitoring haptoglobin concentrations.
- The particular increase in haptoglobin concentrations in serum and peritoneal fluid in cases of postoperative complications suggests a possible role of haptoglobin as not only an inflammation marker but also a potential indicator of postoperative distress in ponies undergoing surgery.
Cite This Article
APA
Eurell TE, Wilson DA, Baker GJ.
(1993).
The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony.
Can J Vet Res, 57(1), 42-44.
Publication
Researcher Affiliations
- College of Veterinary Medicine, University of Illinois, Urbana 61801.
MeSH Terms
- Acute-Phase Reaction / diagnosis
- Acute-Phase Reaction / etiology
- Acute-Phase Reaction / veterinary
- Animals
- Ascitic Fluid / chemistry
- Ascitic Fluid / veterinary
- Female
- Haptoglobins / analysis
- Horse Diseases / diagnosis
- Horse Diseases / etiology
- Horses / blood
- Horses / surgery
- Intestines / surgery
- Intraoperative Period
- Male
- Postoperative Period
References
This article includes 10 references
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Citations
This article has been cited 3 times.- Przewoźny M, Senderska-Płonowska M, Rząsa A, Wierzbicki H, Borkowski J, Swagemakers JH, Żak-Bochenek A, Stefaniak T. Usefulness of Selected Acute-Phase Proteins in the Postsurgical Monitoring of Arthroscopy and Splint Bone Removal in Horses. Animals (Basel) 2021 Oct 13;11(10).
- Taylor S. A review of equine sepsis. Equine Vet Educ 2015 Feb;27(2):99-109.
- Pihl TH, Scheepers E, Sanz M, Goddard A, Page P, Toft N, Andersen PH, Jacobsen S. Influence of disease process and duration on acute phase proteins in serum and peritoneal fluid of horses with colic. J Vet Intern Med 2015 Mar-Apr;29(2):651-8.
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