The effect of metamizole and tolfenamic acid on canine and equine adipose-derived mesenchymal stem cells (ASCs) an in vitro research.

This study investigates how two NSAIDs (Nonsteroidal Anti-inflammatory Drugs): metamizole and tolfenamic acid, affect canine and equine adipose-derived stem cells in vitro, revealing that lower concentrations may stimulate cell activity while higher concentrations can be cytotoxic.

Research Method

• The researchers assessed the influence of two Nonsteroidal Anti-inflammatory Drugs (NSAIDs), metamizole which is a non-selective drug, and tolfenamic acid, which acts selectively, on canine (Ca) and equine (Eq) adipose-derived mesenchymal stem cells (ASCs).
• They monitored the cells’ morphology, ultrastructure and cytophysiological activity under different concentrations of the two drugs.

Lowest Concentration Effects

• The lowest concentration of the drug metamizole (0.01 mg/mL) was found to enhance the viability and cytophysiological activity of canine ASCs, without affecting cell morphology.
• The cells demonstrated an appropriate fibroblastic shape, with large, eccentrically located nuclei.
• Both drugs at this concentration yielded similar effects on equine ASCs as seen in the canine cells.

Intermediate and High Concentrations

• Cells treated with intermediate (0.1 mg/mL) doses of the NSAIDs retained their morphology, but they became more flattened.
• The highest concentrations of the drugs (1 mg/mL) were found to produce cytotoxic effects in both canine and equine ASCs.

Conclusions

• The study concludes that the stimulation of canine and equine ASCs by metamizole and equine ASCs by tolfenamic acid can yield positive effects, but only when the lowest drug concentrations are used.
• The study highlights that different cellular responses are seen in canine and equine ASCs when treated with metamizole and tolfenamic acid. The observed effects at different concentrations become crucial information when considering the use of these drugs in veterinary medicine.

Implications

• The findings from this research may be potentially useful in the investigation of functionalized veterinary biomaterials, especially regarding the safe and effective doses of NSAIDs to apply in treatment protocols involving mesenchymal stem cells.