The Effect of Perfusate Volume on Amikacin Concentration in the Metacarpophalangeal Joint Following Cephalic Regional Limb Perfusion in Standing Horses.
Abstract: To determine the influence of 3 perfusate volumes on amikacin concentration in the metacarpophalangeal joint following cephalic regional limb perfusion (RLP) in standing horses. Methods: Seven healthy horses. Methods: Three perfusate volumes (100, 60, and 30 mL), containing 2 grams of amikacin, were tested during intravenous RLP at the cephalic vein, placing the tourniquet at mid antebrachium, in standing sedated horses. Synovial fluid was collected from the metacarpophalangeal joint before perfusion and at 30 and 120 minutes after perfusion. Serum samples were taken from the jugular vein at the same time points. Samples were analyzed for amikacin concentrations and a repeated measures ANOVA, followed by least squares difference pairwise comparisons to identify differences in amikacin concentration across perfusate volumes. Differences were considered significant at P<.05. Results: The mean amikacin concentration in synovial fluid at 30 minutes after perfusion was significantly higher following perfusate volume of 100 mL (579 μg/mL), compared to volumes of 60 mL (227 μg/mL) or 30 mL (282 μg/mL) (P<.05). When a threshold of 160 μg/mL was used, more horses reached the synovial therapeutic threshold following perfusate volume of 100 mL (100%), than horses receiving 60 mL (43%) and 30 mL (57%) at 30 minutes after injection. Conclusions: The use of 100 mL volume for RLP at the cephalic vein in standing horses resulted in higher concentration of amikacin in the synovial fluid and is recommended for use in clinical cases.
© Copyright 2016 by The American College of Veterinary Surgeons.
Publication Date: 2016-06-08 PubMed ID: 27273831DOI: 10.1111/vsu.12490Google Scholar: Lookup
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- Journal Article
Summary
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The research study investigates the impact of three different volumes of perfusate on the concentration of amikacin in the metacarpophalangeal joint in horses. The study found that higher volumes result in a greater concentration of amikacin in the synovial fluid.
Objective
The study aims to determine how varying volumes (30mL, 60mL, 100mL ) of chosen perfusate carrying 2 grams of antibiotic drug called amikacin can influence its concentration within a particular joint (metacarpophalangeal joint) in horses following a procedure called cephalic regional limb perfusion (RLP).
Methodology
- Seven healthy horses were utilized for the study and three different perfusate volumes were tested.
- Each volume, containing 2 grams of amikacin, was used in a procedure called the regional limb perfusion at the cephalic vein (a necessary vein in a horse’s leg). The process was carried out while the horse stood sedated, with a tourniquet placed at the mid antebrachium (forearm region).
- Samples of synovial fluid, which aids joint movement, were collected from the metacarpophalangeal joint before and at intervals of 30 and 120 minutes after perfusion was administered.
- Simultaneously, serum samples from the jugular vein were also collected at the same time points for analysis.
- These samples were then analysed for amikacin concentrations using a statistical method, including ANOVA and least squares difference pairwise comparisons.
Results and Conclusion
- The analysis showed that the concentration of amikacin was significantly higher 30 minutes after perfusion using the 100mL volume of perfusate (579 μg/mL), as compared to the 60mL (227 μg/mL) and 30mL (282 μg/mL) volumes.
- Furthermore, when tested against a threshold of 160 μg/mL, more horses achieved the therapeutic threshold after perfusion with the 100 mL volume (100%) in comparison to those that received 60 mL (43%) and 30 mL (57%).
- The study concludes that using a 100 mL volume for RLP at the cephalic vein in standing horses resulted in a higher concentration of amikacin in the synovial fluid and therefore it is recommended for use in clinical cases where high amikacin concentration in the joint is ideal.
Cite This Article
APA
Oreff GL, Dahan R, Tatz AJ, Raz T, Britzi M, Kelmer G.
(2016).
The Effect of Perfusate Volume on Amikacin Concentration in the Metacarpophalangeal Joint Following Cephalic Regional Limb Perfusion in Standing Horses.
Vet Surg, 45(5), 625-630.
https://doi.org/10.1111/vsu.12490 Publication
Researcher Affiliations
- Department of Large Animal, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- Department of Large Animal, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- Department of Large Animal, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- Department of Large Animal, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- Kimron Veterinary Institute, National Residue Control Laboratory, Bet Dagan, Israel.
- Department of Large Animal, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
MeSH Terms
- Amikacin / administration & dosage
- Amikacin / pharmacokinetics
- Animals
- Anti-Bacterial Agents / administration & dosage
- Anti-Bacterial Agents / pharmacokinetics
- Dose-Response Relationship, Drug
- Female
- Forelimb / blood supply
- Horses / physiology
- Male
- Metacarpophalangeal Joint / metabolism
- Perfusion / methods
- Perfusion / veterinary
- Synovial Fluid / chemistry
- Tissue Distribution
Citations
This article has been cited 7 times.- O'Brien M, Mochel JP, Kersh K, Wang C, Troy J. Phenylbutazone concentrations in synovial fluid following administration via intravenous regional limb perfusion in the forelimbs of six adult horses. Front Vet Sci 2023;10:1236976.
- Redding LE, Elzer EJ, Ortved KF. Effects of regional limb perfusion technique on concentrations of antibiotic achieved at the target site: A meta-analysis. PLoS One 2022;17(4):e0265971.
- Bonilla AG, Causeret L, Torrent-Crosa A. Pharmacokinetics of ceftiofur in the metacarpophalangeal joint after standing intravenous regional limb perfusion in horses. Can Vet J 2021 Sep;62(9):975-981.
- Gustafsson K, Tatz AJ, Dahan R, Abu Ahmad W, Britzi M, Sutton GA, Kelmer G. Synovial Concentration of Trimethoprim-Sulphadiazine Following Regional Limb Perfusion in Standing Horses. Animals (Basel) 2021 Jul 13;11(7).
- Dahan R, Oreff GL, Tatz AJ, Raz T, Britzi M, Kelmer G. Pharmacokinetics of regional limb perfusion using a combination of amikacin and penicillin in standing horses. Can Vet J 2019 Mar;60(3):294-299.
- Khatibzadeh SM, Dahlgren LA, Caswell CC, Ducker WA, Werre SR, Bogers SH. Equine bone marrow-derived mesenchymal stromal cells reduce established S. aureus and E. coli biofilm matrix in vitro. PLoS One 2024;19(10):e0312917.
- Valverde A, Cribb N, Arroyo L. Morphine concentrations in distal thoracic limb synovial fluid following intravenous regional limb perfusion in horses. Can J Vet Res 2023 Oct;87(4):254-259.
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