The effect of prior thecal puncture on cerebrospinal fluid analytes in normal adult horses.
Abstract: Serial cerebrospinal fluid (CSF) analysis might be required in clinical neurologic disease. The effect of lumbosacral (LS) or cervical (C1-C2) centesis on subsequent CSF cytologic analyses has not been investigated in horses. Objective: To evaluate the effect of thecal puncture on subsequent CSF analyses ANIMALS: Ten healthy adult horses. Methods: Prospective study. Horses were randomly assigned to undergo CSF collection twice, 14 days apart, from either the C1-C2 or LS space. After a 4-month washout period, CSF collection was repeated from the alternate site. Continuous data were analyzed using linear mixed-effects models and count data using mixed-effects negative binomial regression. Statistical significance was set at P < .05. Results: There was no significant effect of collection day (day 0 or day 14) for any CSF analytes, including protein concentration (C1-C2: 45 [95% CI: 33-57] mg/dL day 0 vs 49 [95% CI: 39-62] mg/dL day 14, P = .12; LS: 64 [95% CI: 41-100] mg/dL day 0 vs 83 [95% CI: 53-129] mg/dL day 14, P = .37), or nucleated cell count (C1-C2: 2 [95% CI: 1-4] cells/μL day 0 vs 3 [95% CI: 1-4] cells/μL day 14, P = .65; LS: 3 [95% CI: 2-5] cells/μL day 0 vs 5 [95% CI: 3-8] cells/μL day 14, P = .10). There was no significant difference in EPM titer or EPM serum : CSF ratio between days 0 and 14. Conclusions: Repeat thecal puncture from the LS or C1-C2 space 2 weeks apart does not appear to impact CSF analytes.
© 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC. on behalf of the American College of Veterinary Internal Medicine.
Publication Date: 2020-07-02 PubMed ID: 32614120PubMed Central: PMC7517835DOI: 10.1111/jvim.15842Google Scholar: Lookup
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- Clinical Trial
- Veterinary
- Journal Article
Summary
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The research studied the effect of repeated thecal puncture (spinal tap procedure) on cerebrospinal fluid (CSF) characteristics in horses. The findings suggest that repeated thecal puncture, performed two weeks apart, does not affect CSF analytes, irrespective of whether the procedure is done at the lumbosacral (LS) or cervical (C1-C2) space.
Research Objective and Methodology
- The primary objective of this research was to investigate if thecal punctures (a procedure to collect CSF samples) performed either at the lumbosacral (LS) or cervical (C1-C2) space have an impact on the subsequent CSF analysis in horses.
- The study was carried out using ten healthy adult horses. The selection was random, wherein each horse was subjected to CSF collection twice, with a 14-day interval, either from the C1-C2 or LS space. After a 4-month period, the CSF collection procedure was repeated from the alternate space not initially used.
- Data collected was analyzed through the use of linear mixed-effects models (for continuous data) and mixed-effects negative binomial regression (for count data). A significance level of P < .05 was set for the study.
Results and Findings
- There were no significant changes in the analyzed components of the CSF (analytes), such as protein concentration and nucleated cell count, between day 0 (first collection) and day 14 (second collection), regardless of whether the CSF was collected from C1-C2 or LS space.
- Similarly, there were no significant changes found in Equine Protozoal Myeloencephalitis (EPM) titer or EPM serum : CSF ratio between days 0 and 14.
Conclusions
- Based on the data analysis, the study concluded that thecal punctures performed twice, with a 14-day interval, doesn’t appear to affect CSF analytes irrespective of the area from where CSF is collected, either LS or C1-C2 space.
- This finding can be critical for veterinary neurology as it indicates that repeated thecal punctures for the assessment or monitoring of neurologic diseases should not influence the interpretation of the CSF analysis.
Cite This Article
APA
Chidlow H, Giguère S, Camus M, Wells B, Berghaus R, McConachie Beasley E.
(2020).
The effect of prior thecal puncture on cerebrospinal fluid analytes in normal adult horses.
J Vet Intern Med, 34(5), 2117-2121.
https://doi.org/10.1111/jvim.15842 Publication
Researcher Affiliations
- Department of Large Animal Medicine and Surgery, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA.
- Department of Large Animal Medicine and Surgery, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA.
- Department of Pathology, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA.
- Department of Pathology, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA.
- Department of Population Health, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA.
- Department of Large Animal Medicine and Surgery, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA.
MeSH Terms
- Animals
- Cerebrospinal Fluid
- Horse Diseases
- Horses
- Prospective Studies
- Punctures / veterinary
Grant Funding
- UGA CVM Veterinary Medical Experiment Station
Conflict of Interest Statement
Authors declare no conflict of interest.
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This article includes 17 references
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Citations
This article has been cited 1 times.- Malmström E, Cole RC, Hofmeister EH, Stern JK, Passler T. Comparison of atlantoaxial and lumbosacral cerebrospinal fluid centesis techniques in South American camelids. J Vet Intern Med 2024 Mar-Apr;38(2):1232-1239.
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