The effect of sodium bicarbonate and validation of beckman coulter AU680 analyzers for measuring total carbon dioxide (TCO2) concentrations in horse serum.
Abstract: This study evaluated the usage of Beckman Coulter AU680 analyzers for measurement of TCO in horse serum, and the effect of sodium bicarbonate administrations on serum TCO levels in resting horses. Treatment of horses with sodium bicarbonate did not result in any adverse events. Mean TCO concentration was significantly higher from 1 to 8 h in the sodium bicarbonate-treated horses compared to the untreated controls. Within an hour, administration of sodium bicarbonate increased the TCO level from 31.5 ± -2.5 (SD) to 34.0 ± 2.65 (SD) mmol/L and at 2-8 h post-administration, the TCO level was above the 36 mmol/L cut-off level. In all quality control analysis of Australian standard by Beckman Coulter AU680 analyzer, the instrument slightly over estimated the TCO level but the values were in close agreement with mean TCO level being 38.03 with ± 0.87 mmol/L (SD). Expanded uncertainty was calculated using different levels of confidence interval. Based on 99.5% confidence interval using 0.805% expanded uncertainty using mean measured concentration of 38.05 mmol/L, it was estimated that any race samples TCO level higher than 38.5 mmol/L will be indicative of sodium bicarbonate administration using Beckman Coulter AU680 analyzer in Louisiana.
Publication Date: 2017-10-25 PubMed ID: 29152319PubMed Central: PMC5677778DOI: 10.1002/vms3.82Google Scholar: Lookup
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- Journal Article
Summary
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This research investigated the use of Beckman Coulter AU680 analyzers for accurately measuring the concentration of total carbon dioxide (TCO2) in horse serum, and how administering sodium bicarbonate affects these levels.
Methodology and Objective
- The research aimed to understand the effectiveness and accuracy of Beckman Coulter AU680 analyzers in measuring the concentration of TCO2 in horse serum.
- Simultaneously, the study also aimed to examine the effects of sodium bicarbonate administration on TCO2 levels in horses’ blood serum, primarily focused on resting horses. The duration after administering that these effects would take place was also considered.
Administration of Sodium Bicarbonate
- In this study, sodium bicarbonate was administered to horses and it was observed that there were no adverse events following this injection.
- The concentration of TCO2 was found to be significantly higher from 1 to 8 hours in the horses treated with sodium bicarbonate, compared to those that were untreated.
- Findings showed that within an hour of administration, the levels of TCO2 increased from an average of 31.5 mmol/L to 34.0 mmol/L. From 2-8 hours following administration, TCO2 level exceeded the 36 mmol/L cut-off level indicative of sodium bicarbonate administration.
Quality Control Analysis using Beckman Coulter AU680 Analyzers
- An important part of the study focused on the use of Beckman Coulter AU680 analyzers for measuring TCO2 concentration in horse serum.
- The analyzers, while slightly overestimating the TCO2 levels, still provided values that were in close agreement with the average TCO2 levels – around 38.03 mmol/L. Thus, it was concluded that the analyzer was effective for this purpose.
- The expanded uncertainty was calculated using different levels of confidence intervals. It was deduced that based on 99.5% confidence interval and 0.805% expanded uncertainty, any race samples with TCO2 levels higher than 38.5 mmol/L would indicate sodium bicarbonate administration.
General Conclusions
- The study provided valuable insight into the administration of sodium bicarbonate and its effects on TCO2 levels in horse serum, which can be relevant in equine sports for detecting potential doping.
- Further, it was concluded that Beckman Coulter AU680 analyzers can effectively measure these levels, albeit slightly overestimating them. This tool can be a reliable resource for monitoring these changes in a race environment.
Cite This Article
APA
Dirikolu L, Waller P, Waguespack ML, Andrews FM, Keowen ML, Gaunt SD.
(2017).
The effect of sodium bicarbonate and validation of beckman coulter AU680 analyzers for measuring total carbon dioxide (TCO2) concentrations in horse serum.
Vet Med Sci, 3(4), 263-269.
https://doi.org/10.1002/vms3.82 Publication
Researcher Affiliations
- Equine Medication Surveillance LaboratoryDepartment of Comparative Biomedical SciencesSchool of Veterinary MedicineLouisiana State UniversitySkip Bertman DriveBaton RougeLouisiana70803.
- Equine Medication Surveillance LaboratoryDepartment of Comparative Biomedical SciencesSchool of Veterinary MedicineLouisiana State UniversitySkip Bertman DriveBaton RougeLouisiana70803.
- Equine Medication Surveillance LaboratoryDepartment of Comparative Biomedical SciencesSchool of Veterinary MedicineLouisiana State UniversitySkip Bertman DriveBaton RougeLouisiana70803.
- Equine Health Studies ProgramVeterinary Clinical SciencesLouisiana State University1843 Veterinary Teaching HospitalBaton RougeLouisiana70803.
- Equine Health Studies ProgramVeterinary Clinical SciencesLouisiana State University1843 Veterinary Teaching HospitalBaton RougeLouisiana70803.
- Louisiana Animal Disease Diagnostic LaboratoryDepartment of Pathobiological SciencesSchool of Veterinary MedicineLouisiana State UniversitySkip Bertman Drive Baton RougeLouisiana70803.
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Citations
This article has been cited 1 times.- Ding X, Li M, Peng C, Wang Z, Qian S, Ma Y, Fang T, Feng S, Li Y, Wang X, Li J, Wu J. Uric acid transporters BCRP and MRP4 involved in chickens uric acid excretion. BMC Vet Res 2019 May 30;15(1):180.
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