The effect of supramaximal exercise on equine platelet function.
Abstract: When blood is collected into sodium citrate in the proportion of 9 parts blood:1 part sodium citrate, the concentration of plasma sodium citrate in the sample will depend on the packed cell volume (PCV) of the blood sample. This difference in plasma sodium citrate concentration secondary to alterations in PCV significantly affects human platelet aggregation responses. Since horses attain a high PCV in response to high-intensity exercise we investigated the effect of differences in sample plasma sodium citrate concentration on equine platelet aggregability. In addition, low molecular weight heparin (LMWH) was evaluated as an alternative anticoagulant for assessment of platelet aggregability during strenuous exercise in horses. Blood samples were collected pre-exercise and at fatigue after supramaximal treadmill exercise into either 3.8% sodium citrate (9 parts blood:1 part sodium citrate) or 20 u LMWH/ml of blood. Platelet aggregation responses to 1.25 mumol/l adenosine diphosphate (ADP) were measured via optical aggregometry. For samples collected into sodium citrate, aggregability was significantly less than pre-exercise values in samples collected at fatigue and in pre-exercise samples in which sodium citrate concentrations were adjusted to equal those in fatigue samples. However, samples collected into LMWH showed significantly increased platelet aggregability in samples collected at fatigue when compared to pre-exercise samples. In conclusion, higher plasma sodium citrate concentration had a marked inhibitory effect on equine platelet aggregation responses. Low molecular weight heparin was a good alternative anticoagulant for assessment of equine platelet function and results indicate that equine platelet aggregability was enhanced in response to supramaximal exercise.
Publication Date: 2000-02-05 PubMed ID: 10659248DOI: 10.1111/j.2042-3306.1999.tb05214.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This research investigation analyzes the impact of high-intensity exercise (specifically supramaximal workout) on the function of platelets in horses. It specifically studies the influence of plasma sodium citrate concentration, which can differ due to varying packed cell volumes (PCV) in response to the intensity of exercise. The research also explores the utility of low molecular weight heparin as an alternative anticoagulant for assessment of platelet aggregability.
The Study Purpose and Design
- The purpose of the study was to examine the potential influence of changes in plasma sodium citrate concentration pertaining to variations in PCV on the aggregability of equine platelets. The study was triggered by the fact that horses tend to reach a high PCV level when subjected to intense exercise.
- The research also aimed to evaluate low molecular weight heparin (LMWH) as an alternative anticoagulant for assessing platelet aggregability in horses during intense physical exercises.
- To accomplish these objectives, blood samples were collected from horses before and after performing supramaximal treadmill exercises. The samples were collected into vessels containing either 3.8% sodium citrate or 20 u LMWH/ml of blood.
Evaluation And Findings
- The research measured the amount of platelet aggregation in response to 1.25 mumol/l adenosine diphosphate using optical aggregometry.
- The aggregability of samples collected into sodium citrate significantly dropped, in samples obtained after exercise, compared to pre-exercise samples.
- For samples where sodium citrate levels were adjusted to match those in post-exercise samples, the reduction in platelet aggregability was also observed.
- On the contrary, samples collected into LMWH revealed a significantly increased platelet aggregability in samples collected after exercise compared to pre-exercise samples.
Conclusions Reached From The Study
- The study found that increased plasma sodium citrate concentration led to a conspicuous inhibitory effect on equine platelet aggregation responses.
- Low molecular weight heparin emerged as a promising alternative anticoagulant for assessing equine platelet function.
- The research concluded that intense physical exercise (especially supramaximal workouts) enhanced the aggregability of equine platelets.
Cite This Article
APA
Kingston JK, Sampson SN, Beard LA, Meyers KM, Sellon DC, Bayly WM.
(2000).
The effect of supramaximal exercise on equine platelet function.
Equine Vet J Suppl(30), 181-183.
https://doi.org/10.1111/j.2042-3306.1999.tb05214.x Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164-6610, USA.
MeSH Terms
- Adenosine Diphosphate / pharmacology
- Animals
- Blood Coagulation / drug effects
- Blood Platelets / physiology
- Citrates / blood
- Exercise Test / veterinary
- Hematocrit / veterinary
- Heparin, Low-Molecular-Weight / pharmacology
- Horses / blood
- Horses / physiology
- Physical Conditioning, Animal / physiology
- Platelet Aggregation / drug effects
- Sodium Citrate
Grant Funding
- T35RR07049 / NCRR NIH HHS
Citations
This article has been cited 3 times.- Miglio A, Falcinelli E, Mezzasoma AM, Cappelli K, Mecocci S, Gresele P, Antognoni MT. Effect of First Long-Term Training on Whole Blood Count and Blood Clotting Parameters in Thoroughbreds. Animals (Basel) 2021 Feb 9;11(2).
- Bayly W, Lopez C, Sides R, Bergsma G, Bergsma J, Gold J, Sellon D. Effect of different protocols on the mitigation of exercise-induced pulmonary hemorrhage in horses when administered 24 hours before strenuous exercise. J Vet Intern Med 2019 Sep;33(5):2319-2326.
- Miglio A, Falcinelli E, Cappelli K, Mecocci S, Mezzasoma AM, Antognoni MT, Gresele P. Effect of Regular Training on Platelet Function in Untrained Thoroughbreds. Animals (Basel) 2024 Jan 27;14(3).
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