The effect of systemic administration of cloprostenol on ovulation in mares treated with a prostaglandin synthetase inhibitor.
Abstract: Prostaglandins (PGs) are essential to trigger the cascade of events that degrade the extracellular matrix of follicles leading to follicular rupture and ovulation. In mares, systemic administration of flunixin meglumine (FM), a PG synthetase inhibitor, blocks ovulation by inducing luteinized unruptured follicles (LUF). In the rat, the administration of PGF(2α) (PGF) and PGE restored ovulation in indomethacin treated animals. The mares were treated with FM 0, 12, 24 and 36 h after human chorionic gonadotrophin (hCG) administration to induce experimentally LUF (n = 15) or were left untreated (controls, n = 5). In addition, 250 μg of cloprostenol were administered intravenously to the mares 33, 35 and 36 h (CLO 33, n = 5) or 48, 49 and 50 h (CLO 48, n = 5) after hCG. One group was treated with FM but not with cloprostenol (FM-control, n = 5). The ovulation rate, follicular diameter and progesterone concentration were compared amongst groups. The ovulation rate at 48 h was higher (p < 0.05) in the controls (100%) than in the FM-control (0%), CLO 33 (0%) or CLO 48 (20%) mares. All but one FM treated mares developed LUF by 48 h after hCG administration. Two LUF collapsed between 48 and 60 h and 72 and 84 h in one mare from FM-control and from the CLO 33 group each, respectively. Progesterone concentration was significantly higher (p < 0.05) in the control mares than in any of the FM treated mares 5, 9 and 13 days after hCG. In conclusion, FM administered during the periovulatory period blocked ovulation in the mares. In contrast, the administration of cloprostenol, a PGF analogue, in the previously FM treated mares failed to restore ovulation.
© 2011 Blackwell Verlag GmbH.
Publication Date: 2011-04-20 PubMed ID: 21507082DOI: 10.1111/j.1439-0531.2011.01796.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research paper explores the impact of a prostaglandin synthetase inhibitor (Flunixin Meglumine or FM) and a prostaglandin F2 alpha analogue (cloprostenol) on ovulation in mares. The study found that the administration of FM during the periovulatory period blocks ovulation, while the administration of cloprostenol does not restore ovulation in mares previously treated with FM.
Study Design and Procedure
- Two groups of mares were treated with FM at different intervals (0, 12, 24 and 36 hours) after a human chorionic gonadotropin (hCG) treatment to induce experimentally luteinized unruptured follicles (LUF), while a control group was left untreated.
- In addition to the FM treatment, cloprostenol was administered intravenously at specific times (either 33, 35 and 36 hours or 48, 49 and 50 hours) after the administration of hCG.
- The effect on ovulation rate, follicular diameter, and progesterone concentration in these mares were then observed and compared.
Key Findings
- The rate of ovulation within 48 hours of treatment was found to be significantly higher in the control group than in the groups treated with FM and cloprostenol.
- All but one mare treated with FM developed LUF within 48 hours of treatment, suggesting that FM effectively blocks ovulation.
- The experiment also found that two LUF collapsed between 48-60 hours and 72-84 hours in one mare from the FM-control group and from the cloprostenol grouped at 33 hours respectively.
- Progesterone concentration from 5 to 13 days after hCG treatment was markedly higher in control mares compared to FM-treated mares, implying that FM treatment significantly reduces progesterone levels, which are necessary for successful ovulation and pregnancy.
Conclusion
- The administration of FM during the periovulatory period successfully blocked ovulation in mares.
- Contrarily, the administration of cloprostenol in mares previously treated with FM was not successful in restoring ovulation, suggesting that this prostaglandin analogue’s effects could not reverse the impact of FM.
Cite This Article
APA
Cuervo-Arango J.
(2011).
The effect of systemic administration of cloprostenol on ovulation in mares treated with a prostaglandin synthetase inhibitor.
Reprod Domest Anim, 47(1), 32-38.
https://doi.org/10.1111/j.1439-0531.2011.01796.x Publication
Researcher Affiliations
- Departamento de Medicina y Cirugía Animal, Universidad CEU Cardenal Herrera, Valencia, Spain. juan.cuervo@uch.ceu.es
MeSH Terms
- Animals
- Chorionic Gonadotropin / administration & dosage
- Clonixin / administration & dosage
- Clonixin / analogs & derivatives
- Cloprostenol / administration & dosage
- Cyclooxygenase Inhibitors / administration & dosage
- Female
- Horses / physiology
- Luteinization
- Luteolytic Agents / administration & dosage
- Ovarian Follicle / diagnostic imaging
- Ovarian Follicle / drug effects
- Ovarian Follicle / physiology
- Ovulation / drug effects
- Ovulation Induction / veterinary
- Progesterone / blood
- Ultrasonography
Citations
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