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The effects of aspirin and paracetamol on the aggregation of equine blood platelets.
Abstract: The responses of equine blood platelets in citrated platelet-rich plasma to arachidonic acid, U44069 (prostaglandin endoperoxide analogue), adenosine 5'-diphosphate, platelet-activating factor or collagen were investigated by turbidimetric aggregometry. Pre-treatment of the platelets with aspirin (1 mmol/l) or paracetamol (1.3 mmol/l) abolished shape change and aggregation in response to arachidonic acid; decreased the rate of aggregation in response to collagen, with no separate effect on shape change; had no marked effect on aggregation caused by the other agonists; but in no case transformed irreversible aggregation to reversible aggregation. We conclude that thromboxane A2 generation is of minor importance in the aggregation of equine platelets, and in particular that thromboxane A2 is not a significant mediator of irreversible aggregation.
Publication Date: 1994-10-01 PubMed ID: 7853463DOI: 10.1111/j.1365-2885.1994.tb00262.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research focuses on examining the effects of aspirin and paracetamol on the clotting process, specifically the aggregation (clumping together) of platelets, in horse blood. The study concludes that the generation of thromboxane A2, a compound involved in blood clotting, plays a minor role in equine platelet aggregation, and it does not significantly influence irreversible aggregation.
Study Design and Procedures
- The researchers analyze the reactions of blood platelets hosted in a specific type of plasma (citrated platelet-rich plasma) under the influence of various blood-clotting agents. These include arachidonic acid, adenosine 5′-diphosphate, platelet-activating factor, collagen, and U44069, a synthetic substance modeled after a naturally occurring prostaglandin.
- The procedure encompasses the measurement of platelet aggregation through turbidimetric aggregometry, which involves analyzing light transmission through platelet suspension. This process measures the rate of light blockage due to platelet aggregation.
- The platelets are then pretreated with either aspirin or paracetamol at specific concentrations and their response to the said clotting agents is analyzed.
Results of the Research
- Both aspirin and paracetamol drastically reduce the shape change of platelets and their aggregation when exposed to arachidonic acid.
- The rate of aggregation in response to collagen is likewise affected, albeit without any separate impact on the shape change.
- The two drugs did not significantly alter the aggregation influenced by the other tested agonists.
- In no situation were the drugs found to transform irreversible aggregation into reversible aggregation.
Conclusions of the Research
- Thromboxane A2, a specific type of prostaglandin implicated in blood clotting, contributes minimally to platelet aggregation in horse blood.
- The research further concludes that thromboxane A2 does not considerably mediate irreversible platelet aggregation, thus suggesting that aspirin and paracetamol’s effectiveness in preventing clotting may not be due to their influence on thromboxane A2 levels.
Cite This Article
APA
Heath MF, Evans RJ, Poole AW, Hayes LJ, McEvoy RJ, Littler RM.
(1994).
The effects of aspirin and paracetamol on the aggregation of equine blood platelets.
J Vet Pharmacol Ther, 17(5), 374-378.
https://doi.org/10.1111/j.1365-2885.1994.tb00262.x Publication
Researcher Affiliations
- Department of Clinical Veterinary Medicine, University of Cambridge, UK.
MeSH Terms
- Acetaminophen / administration & dosage
- Acetaminophen / pharmacology
- Adenosine Diphosphate / pharmacology
- Animals
- Arachidonic Acid / pharmacology
- Aspirin / administration & dosage
- Aspirin / pharmacology
- Blood Platelets / drug effects
- Cell Size / drug effects
- Collagen / pharmacology
- Dose-Response Relationship, Drug
- Horses / blood
- In Vitro Techniques
- Nephelometry and Turbidimetry / veterinary
- Platelet Activating Factor / pharmacology
- Platelet Aggregation / drug effects
- Prostaglandin Endoperoxides, Synthetic / pharmacology
- Thromboxane A2 / metabolism
Grant Funding
- Wellcome Trust
Citations
This article has been cited 1 times.- Yilmaz MB, Cihan G, Guray Y, Guray U, Kisacik HL, Sasmaz H, Korkmaz S. Role of mean platelet volume in triagging acute coronary syndromes. J Thromb Thrombolysis 2008 Aug;26(1):49-54.
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