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Home / Equine Research Bank / Vet Res Commun / The effects of furosemide and pentoxifylline on the flow pro...
Veterinary research communications1994; 18(5); 373-381; doi: 10.1007/BF01839288

The effects of furosemide and pentoxifylline on the flow properties of equine erythrocytes: in vitro studies.

Abstract: The effects of various concentrations of furosemide and pentoxifylline on equine RBC in vitro were evaluated to facilitate better understanding of the potential effects of these drugs on blood flow properties. Furosemide induced increased mean cell volume (MCV), increased RBC potassium concentration, increased whole blood viscosity, and decreased the RBC filtrability. These data indicate that furosemide may block the RBC membrane transport pathways resulting in potassium and water retention. The increase in size and the resultant decrease in the surface-area-to-volume ratio may have caused the impaired RBC filtrability and increased blood viscosity. Pentoxifylline improved RBC filtrability without changing the RBC size or the potassium or chloride concentrations, suggesting that pentoxifylline may increase the deformability of the RBC membrane. The study indicated that pentoxifylline has potential therapeutic applications for improving microvascular blood flow but that furosemide may have adverse effects on blood flow.
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Publication Date: 1994-01-01 PubMed ID: 7863609DOI: 10.1007/BF01839288Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the impact of the drugs furosemide and pentoxifylline on the properties of horse red blood cells in a laboratory setting. It was discovered that furosemide could cause changes negatively impacting blood flow, whereas pentoxifylline could potentially improve it.

Study Objectives and Approach

  • The research aimed to study the potential effects of different concentrations of furosemide and pentoxifylline on the properties of red blood cells (RBCs) in horses.
  • This investigation was approached from in vitro or laboratory conditions, which means that it was conducted outside of a living organism.
  • The effects of these drugs on RBC characteristics such as volume, potassium concentration, blood viscosity, and RBC filtrability were the primary concerns.

Key Findings

  • Furosemide was found to increase the mean cell volume, RBC potassium concentration, and the viscosity of whole blood. It also reduced the RBC filtrability.
  • This suggests that furosemide may interfere with (or “block”) the pathways across the red blood cell membrane, causing potassium and water to be retained in the cell.
  • The expanded size of cells and reduced surface-area-to-volume ratio may be responsible for the decreased RBC filtrability and increased blood viscosity.
  • On the other hand, pentoxifylline improved the RBC’s ability to be filtered without causing changes in its size or concentrations of potassium and chloride molecules.
  • This suggests that pentoxifylline may enhance the deformability (flexibility) of the red blood cell membrane.

Research Implications

  • This study highlights potential therapeutic applications of pentoxifylline for improving microvascular blood flow, owing to its effects on red blood cell characteristics.
  • In contrast, the use of furosemide might have adverse effects on blood flow, presumably due to how it impacts red blood cell properties and behaviours.
  • This study provides valuable insights to guide medical decisions for equine health, particularly regarding drug implications for their blood flow and cell characteristics.

Cite This Article

APA
Weiss DJ, Evanson OA, Geor RJ. (1994). The effects of furosemide and pentoxifylline on the flow properties of equine erythrocytes: in vitro studies. Vet Res Commun, 18(5), 373-381. https://doi.org/10.1007/BF01839288

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 18
Issue: 5
Pages: 373-381

Researcher Affiliations

Weiss, D J
  • Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Evanson, O A
    Geor, R J

      MeSH Terms

      • Animals
      • Blood Viscosity / drug effects
      • Erythrocyte Indices / drug effects
      • Erythrocyte Indices / veterinary
      • Erythrocytes / chemistry
      • Erythrocytes / drug effects
      • Erythrocytes / physiology
      • Furosemide / pharmacology
      • Horses / blood
      • Pentoxifylline / pharmacology
      • Potassium / analysis
      • Regional Blood Flow

      References

      This article includes 22 references
      1. Tsukimoto K, Mathieu-Costello O, Prediletto R, Elliott AR, West JB. Ultrastructural appearances of pulmonary capillaries at high transmural pressures.. J Appl Physiol (1985) 1991 Aug;71(2):573-82.
        pubmed: 1718936doi: 10.1152/jappl.1991.71.2.573google scholar: lookup
      2. McClay CB, Weiss DJ, Smith CM 2nd, Gordon B. Evaluation of hemorheologic variables as implications for exercise-induced pulmonary hemorrhage in racing thoroughbreds.. Am J Vet Res 1992 Aug;53(8):1380-5.
        pubmed: 1510314
      3. Geor RJ, Weiss DJ, Burris SM, Smith CM 2nd. Effects of furosemide and pentoxifylline on blood flow properties in horses.. Am J Vet Res 1992 Nov;53(11):2043-9.
        pubmed: 1466500
      4. Manohar M, Hutchens E, Coney E. Frusemide attenuates the exercise-induced rise in pulmonary capillary blood pressure in horses.. Equine Vet J 1994 Jan;26(1):51-4.
        pubmed: 8143666doi: 10.1111/j.2042-3306.1994.tb04331.xgoogle scholar: lookup
      5. Ambrus JL, Anain JM, Anain SM, Anain PM, Anain JM Jr, Stadler S, Mitchell P, Brobst JA, Cobert BL, Savitsky JP. Dose-response effects of pentoxifylline on erythrocyte filterability: clinical and animal model studies.. Clin Pharmacol Ther 1990 Jul;48(1):50-6.
        pubmed: 2196145doi: 10.1038/clpt.1990.117google scholar: lookup
      6. Weiss DJ, Geor RJ, Burris SM, Smith CM 2nd. Effects of pentoxifylline on equine neutrophil function and flow properties.. Can J Vet Res 1992 Oct;56(4):313-7.
        pubmed: 1335832
      7. Coyne CP, Carlson GP, Spensley MS, Smith J. Preliminary investigation of alterations in blood viscosity, cellular composition, and electrophoresis plasma protein fraction profile after competitive racing activity in Thoroughbred horses.. Am J Vet Res 1990 Dec;51(12):1956-63.
        pubmed: 2085222
      8. Andrews FM, Korenek NL, Sanders WL, Hamlin RL. Viscosity and rheologic properties of blood from clinically normal horses.. Am J Vet Res 1992 Jun;53(6):966-70.
        pubmed: 1626789
      9. Manohar M. Furosemide attenuates the exercise-induced increase in pulmonary artery wedge pressure in horses.. Am J Vet Res 1993 Jun;54(6):952-8.
        pubmed: 8323066
      10. Manohar M. Pulmonary artery wedge pressure increases with high-intensity exercise in horses.. Am J Vet Res 1993 Jan;54(1):142-6.
        pubmed: 8427458
      11. Hinchcliff KW, Muir WW 3rd. Pharmacology of furosemide in the horse: a review.. J Vet Intern Med 1991 Jul-Aug;5(4):211-8.
        pubmed: 1941755doi: 10.1111/j.1939-1676.1991.tb00951.xgoogle scholar: lookup
      12. Beermann B, Ings R, Månsby J, Chamberlain J, McDonald A. Kinetics of intravenous and oral pentoxifylline in healthy subjects.. Clin Pharmacol Ther 1985 Jan;37(1):25-8.
        pubmed: 3965236doi: 10.1038/clpt.1985.6google scholar: lookup
      13. Crisman MV, Wilcke JR, Correll LS, Irby MH. Pharmacokinetic disposition of intravenous and oral pentoxifylline in horses.. J Vet Pharmacol Ther 1993 Mar;16(1):23-31.
        pubmed: 8478997doi: 10.1111/j.1365-2885.1993.tb00285.xgoogle scholar: lookup
      14. Ellory JC, Hall AC, Ody SO, Englert HC, Mania D, Lang HJ. Selective inhibitors of KCl cotransport in human red cells.. FEBS Lett 1990 Mar 26;262(2):215-8.
        pubmed: 2335202doi: 10.1016/0014-5793(90)80193-mgoogle scholar: lookup
      15. Serrani RE, Corchs JL. Furosemide-sensitive sodium and potassium fluxes in human neonatal erythrocytes.. Arch Int Physiol Biochim 1987 Nov;95(4):341-6.
        pubmed: 2453178
      16. Berkowitz LR. Loop diuretic and anion modification of NEM-induced K transport in human red blood cells.. Am J Physiol 1990 Apr;258(4 Pt 1):C622-9.
        pubmed: 2333949doi: 10.1152/ajpcell.1990.258.4.C622google scholar: lookup
      17. Clemens MR, Ruess M. Effect of pentoxifylline on erythrocyte membrane and plasma lipids.. Eur J Clin Pharmacol 1991;41(6):623-4.
        pubmed: 1815980doi: 10.1007/BF00315000google scholar: lookup
      18. Weiss DJ, Geor R, Smith CM 2nd, McClay CB. Furosemide-induced electrolyte depletion associated with echinocytosis in horses.. Am J Vet Res 1992 Oct;53(10):1769-72.
        pubmed: 1456517
      19. O'Callaghan MW, Pascoe JR, Tyler WS, Mason DK. Exercise-induced pulmonary haemorrhage in the horse: results of a detailed clinical, post mortem and imaging study. IV. Changes in the bronchial circulation demonstrated by C.T. scanning and microradiography.. Equine Vet J 1987 Sep;19(5):405-10.
        pubmed: 3678182doi: 10.1111/j.2042-3306.1987.tb02631.xgoogle scholar: lookup
      20. Weiss DJ, Geor RJ, Smith CM 2nd. Effects of echinocytosis on hemorrheologic values and exercise performance in horses.. Am J Vet Res 1994 Feb;55(2):204-10.
        pubmed: 8172408
      21. Contreras A, Martínez R, Devés R, Marusic ET. An unusual pattern of Na+ and K+ movements across the horse erythrocyte membrane.. Biochim Biophys Acta 1986 Apr 14;856(2):388-91.
        pubmed: 3006774doi: 10.1016/0005-2736(86)90050-7google scholar: lookup
      22. Fu Z, Costello ML, Tsukimoto K, Prediletto R, Elliott AR, Mathieu-Costello O, West JB. High lung volume increases stress failure in pulmonary capillaries.. J Appl Physiol (1985) 1992 Jul;73(1):123-33.
        pubmed: 1506359doi: 10.1152/jappl.1992.73.1.123google scholar: lookup

      Citations

      This article has been cited 0 times.
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