The effects of pyrantel tartrate on Sarcocystis neurona merozoite viability.
Abstract: Sarcocystis neurona is the etiologic agent of equine protozoal myeloencephalitis, a neurologic disease of horses. The present study was designed to test the hypothesis that pyrantel tartrate can kill S. neurona merozoites growing in equine dermal cell culture. Sarcocystis neurona merozoites were exposed to a range of concentrations of pyrantel tartrate or sodium tartrate ranging from 0.001 to 0.01 M. Merozoites were then placed onto equine dermal cell cultures and incubated for 2 weeks to check for viability. At 1 and 2 weeks after inoculation, plaque counts were compared between treatments and, between treatments and controls. Merozoites exposed to concentrations of pyrantel tartrate higher than 0.0025 M (8.91 x 10(-4) g/ml) did not produce plaques in equine dermal cells, whereas those exposed to similar concentrations of the tartrate salt or medium alone produced significant numbers of plaques. These results demonstrate that pyrantel tartrate has activity against S. neurona merozoites in vitro and suggest that it may have activity against the sporozoite stage of the parasite found in the equine gut.
Publication Date: 2001-07-01 PubMed ID: 19746670
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research examines the potential of the drug pyrantel tartrate to kill Sarcocystis neurona merozoites, a cause of horse neurological disease, when grown in horse skin cell culture.
Objective of the Research
- The research was done to test if the drug pyrantel tartrate can destroy Sarcocystis neurona merozoites, the cause of a severe neurological disease in horses known as equine protozoal myeloencephalitis. The testing was conducted on merozoites developing in equine dermal (skin) cell culture.
Methods of the Research
- In the experiment, S. neurona merozoites were exposed to varying levels of pyrantel tartrate or sodium tartrate, ranging from 0.001 to 0.01 M.
- Subsequently, these merozoites were infused into equine dermal cell cultures and left to incubate for a period of two weeks. This was done to check whether they remained viable.
Results of the Research
- One and two weeks following the inoculation, the count of plaques (presumably an indication of merozoite viability and replication) was compared across the different treatments, and between the treatments and controls.
- The findings pointed out that merozoites exposed to pyrantel tartrate concentrations higher than 0.0025 M (8.91 x 10(-4) g/ml) did not produce plaques in the equine dermal cells. It means that this concentration of pyrantel tartrate managed to kill the merozoites.
- On the other hand, merozoites in contact with similar levels of tartrate salt or medium alone showed significant numbers of plaques, indicating that these conditions did not inhibit the merozoites.
Conclusion of the Research
- The experiment showed that pyrantel tartrate can act against S. neurona merozoites in vitro, meaning outside of a living organism. This outcome suggests the drug might also be effective against the sporozoite stage of the parasite, which is found in the horse’s gut.
Cite This Article
APA
Kruttlin EA, Rossano MG, Murphy AJ, Vrable RA, Kaneene JB, Schott HC, Mansfield LS.
(2001).
The effects of pyrantel tartrate on Sarcocystis neurona merozoite viability.
Vet Ther, 2(3), 268-276.
Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, D201 Veterinary Medicine Center, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
MeSH Terms
- Animals
- Antiprotozoal Agents / pharmacology
- Cell Line
- Dose-Response Relationship, Drug
- Horses
- Merozoites / drug effects
- Pyrantel Tartrate / pharmacology
- Sarcocystis / drug effects
Citations
This article has been cited 3 times.- Bowden GD, Land KM, O'Connor RM, Fritz HM. High-throughput screen of drug repurposing library identifies inhibitors of Sarcocystis neurona growth.. Int J Parasitol Drugs Drug Resist 2018 Apr;8(1):137-144.
- Dubey JP, Howe DK, Furr M, Saville WJ, Marsh AE, Reed SM, Grigg ME. An update on Sarcocystis neurona infections in animals and equine protozoal myeloencephalitis (EPM).. Vet Parasitol 2015 Apr 15;209(1-2):1-42.
- Elsheikha HM, Murphy AJ, Mansfield LS. Phylogenetic congruence of Sarcocystis neurona Dubey et al., 1991 (Apicomplexa: Sarcocystidae) in the United States based on sequence analysis and restriction fragment length polymorphism (RFLP).. Syst Parasitol 2005 Jul;61(3):191-202.
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