The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection.
Abstract: The most used and reliable indicator of Equine infectious anemia virus (EIAV) infection is the detection of its specific antibodies in horse serum. In the present study, the performance of two commercial ELISA tests for the detection of EIAV antibodies as well as the potential advantages of their use as an EIAV infection screening tool were evaluated in 302 horse serum samples. Both ELISA assays showed 100% diagnostic sensitivity, and 92.3-94.3% diagnostic specificity. Discordant results were analyzed by immunoblot. The results showed that both ELISA tests are very efficient at detecting EIAV infected animals, allowing to identify a higher number of positive horse cases. Thus, ELISA assays can be useful tools in EIA control and eradication.
Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.
Publication Date: 2015-02-28 PubMed ID: 25735214DOI: 10.1016/j.ram.2014.12.001Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Antibodies
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Health
- Equine Infectious Anemia
- Horses
- Immunology
- Infection
- Infectious Disease
- Laboratory Methods
- Serology
- Veterinary Care
- Veterinary Medicine
- Veterinary Research
- Virus
Summary
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This research study explored the effectiveness of two commercial ELISA tests in identifying Equine infectious anemia virus (EIAV) in horse serum samples, discovering high accuracy and sensitivity in detecting the virus, thereby promoting their use for disease control and eradication.
Overview of the Research
- The study aimed to verify the performance of two commercial ELISA (Enzyme-Linked Immunosorbent Assay) tests in the detection of EIAV (Equine infectious anemia virus) antibodies.
- EIAV is a retrovirus that infects horses and causes anemia, intermittent fever, and severe medical conditions. Identifying this virus in its early stages is essential for the horse’s health.
- The tests were administered on 302 horse serum samples as part of the study.
Evaluation of ELISA Tests
- Both ELISA tests showcased 100% diagnostic sensitivity, meaning they were able to correctly identify all samples that did have EIAV.
- The tests also exhibited a diagnostic specificity between 92.3-94.3%, indicating they could correctly identify those samples without the virus at a high rate. This is an important measure in ensuring that non-infected horses aren’t mistakenly identified as infected.
Analyses of Discordant Results
- Discordant results, the few instances where the ELISA test results did not agree, were analyzed with immunoblotting, a technique used to detect specific proteins in a sample.
- The research doesn’t specify the outcomes of this analysis, but it denotes there was an additional step to interpret any conflicting results.
Advantages of Using ELISA Tests
- The study suggests that ELISA tests can help detect higher numbers of EIAV positive cases due to their high diagnostic sensitivity and specificity.
- Due to their productivity, these tests play a significant role in developing strategies for controlling and eradicating EIA. They can be used in surveillance programs that aim to prevent the spread of EIA among horses.
Cite This Article
APA
Alvarez I, Cipolini F, Wigdorovitz A, Trono K, Barrandeguy ME.
(2015).
The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection.
Rev Argent Microbiol, 47(1), 25-28.
https://doi.org/10.1016/j.ram.2014.12.001 Publication
Researcher Affiliations
- Instituto de Virología, CICVyA, INTA, Hurlingham, Buenos Aires, Argentina; CONICET, Argentina. Electronic address: alvarez.irene@inta.gob.ar.
- Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, Corrientes, Argentina.
- Instituto de Virología, CICVyA, INTA, Hurlingham, Buenos Aires, Argentina; CONICET, Argentina.
- Instituto de Virología, CICVyA, INTA, Hurlingham, Buenos Aires, Argentina.
- Instituto de Virología, CICVyA, INTA, Hurlingham, Buenos Aires, Argentina; Escuela de Veterinaria, Universidad del Salvador, Pilar, Buenos Aires, Argentina.
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Enzyme-Linked Immunosorbent Assay
- Equine Infectious Anemia / diagnosis
- Horses
- Infectious Anemia Virus, Equine / immunology
- Reagent Kits, Diagnostic
Citations
This article has been cited 1 times.- Idris SM, Eltom KH, Okuni JB, Ojok L, Elmagzoub WA, El Wahed AA, Eltayeb E, Gameel AA. Paratuberculosis: The Hidden Killer of Small Ruminants.. Animals (Basel) 2021 Dec 21;12(1).
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