The equine herpesvirus type 1 glycoprotein homologous to herpes simplex virus type 1 glycoprotein M is a major constituent of the virus particle.
Abstract: Glycoprotein 45 is a major envelope glycoprotein of equine herpesvirus type 1. The gene encoding this protein is located between map units 0.615 and 0.636 on the virus genome and evidence has suggested that it is encoded by gene 52, one of four genes within this region. Using PCR we have amplified gene 52 and subsequently cloned it into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. The gene was expressed in COS-7 cells and its product was detected by immunofluorescence and Western blotting. The results indicate that glycoprotein 45 is encoded by gene 52, and that it is the homologue of herpes simplex virus type 1 glycoprotein M.
Publication Date: 1994-02-01 PubMed ID: 8113768DOI: 10.1099/0022-1317-75-2-439Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the equine herpesvirus type 1 and identifies a major envelope glycoprotein in it called Glycoprotein 45, which is encoded by gene 52 and found to be the homologue of herpes simplex virus type 1 glycoprotein M.
About the Research
- Conducted on the equine herpesvirus type 1, the study mainly focuses on unveiling and understanding one of its most important constituents, named Glycoprotein 45.
- Location of this glycoprotein gene is situated between map units 0.615 and 0.636 of the virus genome. There are indications that it might be encoded by gene 52, one among the four genes tucked within the mentioned region.
Method of Research
- The researchers employ Polymerase Chain Reaction (PCR) to amplify gene 52, which presumably encodes the glycoprotein 45.
- Subsequently, the amplified gene 52 was cloned into a mammalian expression vector. This process was subjected to the regulation of the human cytomegalovirus immediate early gene promoter.
Results and Findings
- The researchers proceeded with the expression of gene 52 in COS-7 cells. The expression product was then detected and identified through processes like immunofluorescence and Western blotting.
- The outcome of the study indicates that Glycoprotein 45 is encoded by gene 52. Moreover, it also reveals that this glycoprotein is essentially the homologue of herpes simplex virus type 1, glycoprotein M.
Cite This Article
APA
Pilling A, Davison AJ, Telford EA, Meredith DM.
(1994).
The equine herpesvirus type 1 glycoprotein homologous to herpes simplex virus type 1 glycoprotein M is a major constituent of the virus particle.
J Gen Virol, 75 ( Pt 2), 439-442.
https://doi.org/10.1099/0022-1317-75-2-439 Publication
Researcher Affiliations
- Department of Microbiology, University of Leeds, U.K.
MeSH Terms
- Base Sequence
- Cells, Cultured
- Genes, Viral
- Herpesvirus 1, Equid / genetics
- Herpesvirus 1, Human / genetics
- Molecular Sequence Data
- Polymerase Chain Reaction
- Transfection
- Viral Envelope Proteins / genetics
Citations
This article has been cited 10 times.- Van Cleemput J, Poelaert KCK, Laval K, Vanderheijden N, Dhaenens M, Daled S, Boyen F, Pasmans F, Nauwynck HJ. An Alphaherpesvirus Exploits Antimicrobial β-Defensins To Initiate Respiratory Tract Infection.. J Virol 2020 Mar 31;94(8).
- Yamagishi Y, Sadaoka T, Yoshii H, Somboonthum P, Imazawa T, Nagaike K, Ozono K, Yamanishi K, Mori Y. Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread.. J Virol 2008 Jan;82(2):795-804.
- Molinková D, Celer V Jr, Jahn P. Isolation and partial characterization of equine herpesvirus type 1 in Czechia.. Folia Microbiol (Praha) 2004;49(5):605-11.
- Mach M, Kropff B, Dal Monte P, Britt W. Complex formation by human cytomegalovirus glycoproteins M (gpUL100) and N (gpUL73).. J Virol 2000 Dec;74(24):11881-92.
- Isegawa Y, Mukai T, Nakano K, Kagawa M, Chen J, Mori Y, Sunagawa T, Kawanishi K, Sashihara J, Hata A, Zou P, Kosuge H, Yamanishi K. Comparison of the complete DNA sequences of human herpesvirus 6 variants A and B.. J Virol 1999 Oct;73(10):8053-63.
- Lake CM, Molesworth SJ, Hutt-Fletcher LM. The Epstein-Barr virus (EBV) gN homolog BLRF1 encodes a 15-kilodalton glycoprotein that cannot be authentically processed unless it is coexpressed with the EBV gM homolog BBRF3.. J Virol 1998 Jul;72(7):5559-64.
- Wu SX, Zhu XP, Letchworth GJ. Bovine herpesvirus 1 glycoprotein M forms a disulfide-linked heterodimer with the U(L)49.5 protein.. J Virol 1998 Apr;72(4):3029-36.
- Dijkstra JM, Visser N, Mettenleiter TC, Klupp BG. Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component.. J Virol 1996 Aug;70(8):5684-8.
- Nicholas J. Determination and analysis of the complete nucleotide sequence of human herpesvirus.. J Virol 1996 Sep;70(9):5975-89.
- Osterrieder N, Neubauer A, Brandmuller C, Braun B, Kaaden OR, Baines JD. The equine herpesvirus 1 glycoprotein gp21/22a, the herpes simplex virus type 1 gM homolog, is involved in virus penetration and cell-to-cell spread of virions.. J Virol 1996 Jun;70(6):4110-5.
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