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Animals : an open access journal from MDPI2019; 9(8); doi: 10.3390/ani9080579

The First Identification and Antibiogram of Clostridium perfringens Type C Isolated from Soil and The Feces of Dead Foals in South Korea.

Abstract: Clostridium (C.) perfringens was isolated from 25 (11.1%) of 225 sampled horses and from 16 (35.56%) of 45 farms. All of the samples were negative for cpe, etx, itx, NetF genes and cpa gene were detected in 100% (25 of 25) of the samples that were positive for C. perfringens. cpb and cpb2 were detected in 40.0% (10 of 25) and 60.0% (15 of 25) of the samples that were positive for C. perfringens, respectively. Of the 25 C. perfringens isolates, 15 (60%) were type A and 10 (40%) were type C. Type C was observed on all the farms where the foals' deaths occurred. None of the isolates were positive for type B, type D, or type E. The MIC Evaluator strips antimicrobial susceptibility test showed meropenem (96%), ampicillin (92%), amoxicillin/clavulanic acid (84%), and tetracycline (8%) sensitivity.
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Publication Date: 2019-08-20 PubMed ID: 31434197PubMed Central: PMC6719196DOI: 10.3390/ani9080579Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research work focuses on the identification and antibiotic sensitivity assessment of the Clostridium perfringens bacteria Type C, found in soil and dead foal feces in South Korea.

Overview and Methodology

  • This research was undertaken to identify the presence and assess the antibiotic resistance of C. perfringens bacteria in horses and farms across South Korea. Samples were taken from 225 horses across 45 farms.
  • Genetic testing was performed on the isolated samples to determine the presence of various genes associated with specific types of C. perfringens – cpe, ect, itx, NetF, and cpa.

Results of the Study

  • C. perfringens bacteria was detected in 11.1% or 25 of the 225 horses and on 35.56% or 16 of the 45 farms that were sampled.
  • No samples came out positive for cpe, etx, itx, NetF genes while cpa gene was found in all (100%) 25 positive samples of C. perfringens.
  • The genes cpb and cpb2 were found in 40.0% (10 of 25) and 60.0% (15 of 25) of the positive samples, respectively.

Types of C. perfringens and Antibiotic Sensitivity

  • Out of 25 C. perfringens isolates, 15 (60%) were type A and 10 (40%) were Type C. Type C was present on all farms where there were reported foal deaths. None of the isolated strains were type B, D, or E.
  • A test for antibiotic susceptibility showed varying sensitivity to different antibiotics. The majority of the bacteria were sensitive to meropenem (96%) and ampicillin (92%), and to a lesser extent amoxicillin/clavulanic acid (84%), and only 8% were sensitive to tetracycline.

Cite This Article

APA
Park CS, Hwang JY, Cho GJ. (2019). The First Identification and Antibiogram of Clostridium perfringens Type C Isolated from Soil and The Feces of Dead Foals in South Korea. Animals (Basel), 9(8). https://doi.org/10.3390/ani9080579

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 9
Issue: 8

Researcher Affiliations

Park, Chul Song
  • Laboratory of Equine Medicine, College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 41566, Korea.
Hwang, Ji Yong
  • Laboratory of Equine Medicine, College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 41566, Korea.
Cho, Gil Jae
  • Laboratory of Equine Medicine, College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 41566, Korea. chogj@knu.ac.kr.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 4 times.
  1. Li J, Xie K, Yang J, Zhang J, Yang Q, Wang P, Gun S, Huang X. S100A9 plays a key role in Clostridium perfringens beta2 toxin-induced inflammatory damage in porcine IPEC-J2 intestinal epithelial cells. BMC Genomics 2023 Jan 12;24(1):16.
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