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Animals : an open access journal from MDPI2019; 9(8); doi: 10.3390/ani9080579

The First Identification and Antibiogram of Clostridium perfringens Type C Isolated from Soil and The Feces of Dead Foals in South Korea.

Abstract: Clostridium (C.) perfringens was isolated from 25 (11.1%) of 225 sampled horses and from 16 (35.56%) of 45 farms. All of the samples were negative for cpe, etx, itx, NetF genes and cpa gene were detected in 100% (25 of 25) of the samples that were positive for C. perfringens. cpb and cpb2 were detected in 40.0% (10 of 25) and 60.0% (15 of 25) of the samples that were positive for C. perfringens, respectively. Of the 25 C. perfringens isolates, 15 (60%) were type A and 10 (40%) were type C. Type C was observed on all the farms where the foals' deaths occurred. None of the isolates were positive for type B, type D, or type E. The MIC Evaluator strips antimicrobial susceptibility test showed meropenem (96%), ampicillin (92%), amoxicillin/clavulanic acid (84%), and tetracycline (8%) sensitivity.
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Publication Date: 2019-08-20 PubMed ID: 31434197PubMed Central: PMC6719196DOI: 10.3390/ani9080579Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research work focuses on the identification and antibiotic sensitivity assessment of the Clostridium perfringens bacteria Type C, found in soil and dead foal feces in South Korea.

Overview and Methodology

  • This research was undertaken to identify the presence and assess the antibiotic resistance of C. perfringens bacteria in horses and farms across South Korea. Samples were taken from 225 horses across 45 farms.
  • Genetic testing was performed on the isolated samples to determine the presence of various genes associated with specific types of C. perfringens – cpe, ect, itx, NetF, and cpa.

Results of the Study

  • C. perfringens bacteria was detected in 11.1% or 25 of the 225 horses and on 35.56% or 16 of the 45 farms that were sampled.
  • No samples came out positive for cpe, etx, itx, NetF genes while cpa gene was found in all (100%) 25 positive samples of C. perfringens.
  • The genes cpb and cpb2 were found in 40.0% (10 of 25) and 60.0% (15 of 25) of the positive samples, respectively.

Types of C. perfringens and Antibiotic Sensitivity

  • Out of 25 C. perfringens isolates, 15 (60%) were type A and 10 (40%) were Type C. Type C was present on all farms where there were reported foal deaths. None of the isolated strains were type B, D, or E.
  • A test for antibiotic susceptibility showed varying sensitivity to different antibiotics. The majority of the bacteria were sensitive to meropenem (96%) and ampicillin (92%), and to a lesser extent amoxicillin/clavulanic acid (84%), and only 8% were sensitive to tetracycline.

Cite This Article

APA
Park CS, Hwang JY, Cho GJ. (2019). The First Identification and Antibiogram of Clostridium perfringens Type C Isolated from Soil and The Feces of Dead Foals in South Korea. Animals (Basel), 9(8). https://doi.org/10.3390/ani9080579

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 9
Issue: 8

Researcher Affiliations

Park, Chul Song
  • Laboratory of Equine Medicine, College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 41566, Korea.
Hwang, Ji Yong
  • Laboratory of Equine Medicine, College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 41566, Korea.
Cho, Gil Jae
  • Laboratory of Equine Medicine, College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 41566, Korea. chogj@knu.ac.kr.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 33 references
  1. Deguchi A, Miyamoto K, Kuwahara T, Miki Y, Kaneko I, Li J, McClane BA, Akimoto S. Genetic characterization of type A enterotoxigenic Clostridium perfringens strains.. PLoS One 2009 May 19;4(5):e5598.
    doi: 10.1371/journal.pone.0005598pmc: PMC2682570pubmed: 19479065google scholar: lookup
  2. Diab SS, Kinde H, Moore J, Shahriar MF, Odani J, Anthenill L, Songer G, Uzal FA. Pathology of Clostridium perfringens type C enterotoxemia in horses.. Vet Pathol 2012 Mar;49(2):255-63.
    doi: 10.1177/0300985811404710pubmed: 21502373google scholar: lookup
  3. Li J, Adams V, Bannam TL, Miyamoto K, Garcia JP, Uzal FA, Rood JI, McClane BA. Toxin plasmids of Clostridium perfringens.. Microbiol Mol Biol Rev 2013 Jun;77(2):208-33.
    doi: 10.1128/MMBR.00062-12pmc: PMC3668675pubmed: 23699255google scholar: lookup
  4. Nagahama M, Ochi S, Oda M, Miyamoto K, Takehara M, Kobayashi K. Recent insights into Clostridium perfringens beta-toxin.. Toxins (Basel) 2015 Feb 3;7(2):396-406.
    doi: 10.3390/toxins7020396pmc: PMC4344631pubmed: 25654787google scholar: lookup
  5. Othani K, Shimizu T. Regulation of Toxin Production in Clostridium perfringens. Toxins 2016;8:207.
  6. Uzal FA, Vidal JE, McClane BA, Gurjar AA. Clostridium Perfringens Toxins Involved in Mammalian Veterinary Diseases.. Open Toxinology J 2010;2:24-42.
    doi: 10.2174/1875414701003010024pmc: PMC3917546pubmed: 24511335google scholar: lookup
  7. Bokori-Brown M, Savva CG, Fernandes da Costa SP, Naylor CE, Basak AK, Titball RW. Molecular basis of toxicity of Clostridium perfringens epsilon toxin.. FEBS J 2011 Dec;278(23):4589-601.
    doi: 10.1111/j.1742-4658.2011.08140.xpubmed: 21518257google scholar: lookup
  8. Nakano V, Ignacio A, Llanco L, Bueris V, Sircili MP, Avila-Campos MJ. Multilocus sequence typing analyses of Clostridium perfringens type A strains harboring tpeL and netB genes.. Anaerobe 2017 Apr;44:99-105.
    doi: 10.1016/j.anaerobe.2017.02.017pubmed: 28238845google scholar: lookup
  9. Coursodon CF, Glock RD, Moore KL, Cooper KK, Songer JG. TpeL-producing strains of Clostridium perfringens type A are highly virulent for broiler chicks.. Anaerobe 2012 Feb;18(1):117-21.
    doi: 10.1016/j.anaerobe.2011.10.001pubmed: 22019986google scholar: lookup
  10. Songer JG. Clostridial enteric diseases of domestic animals.. Clin Microbiol Rev 1996 Apr;9(2):216-34.
    doi: 10.1128/CMR.9.2.216pmc: PMC172891pubmed: 8964036google scholar: lookup
  11. Gkiourtzidis K, Frey J, Bourtzi-Hatzopoulou E, Iliadis N, Sarris K. PCR detection and prevalence of alpha-, beta-, beta 2-, epsilon-, iota- and enterotoxin genes in Clostridium perfringens isolated from lambs with clostridial dysentery.. Vet Microbiol 2001 Sep 3;82(1):39-43.
    doi: 10.1016/S0378-1135(01)00327-3pubmed: 11423193google scholar: lookup
  12. Klaasen HL, Molkenboer MJ, Bakker J, Miserez R, Häni H, Frey J, Popoff MR, van den Bosch JF. Detection of the beta2 toxin gene of Clostridium perfringens in diarrhoeic piglets in The Netherlands and Switzerland.. FEMS Immunol Med Microbiol 1999 Jul;24(3):325-32.
    pubmed: 10397318doi: 10.1111/j.1574-695x.1999.tb01301.xgoogle scholar: lookup
  13. Mahony DE, Clark GA, Stringer MF, MacDonald MC, Duchesne DR, Mader JA. Rapid extraction of plasmids from Clostridium perfringens.. Appl Environ Microbiol 1986 Mar;51(3):521-3.
    pmc: PMC238912pubmed: 2870680doi: 10.1128/aem.51.3.521-523.1986google scholar: lookup
  14. Chalmers G, Bruce HL, Hunter DB, Parreira VR, Kulkarni RR, Jiang YF, Prescott JF, Boerlin P. Multilocus sequence typing analysis of Clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations.. J Clin Microbiol 2008 Dec;46(12):3957-64.
    doi: 10.1128/JCM.01548-08pmc: PMC2593256pubmed: 18945840google scholar: lookup
  15. Howard-Martin M, Morton RJ, Qualls CW Jr, MacAllister CG. Clostridium perfringens type C enterotoxemia in a newborn foal.. J Am Vet Med Assoc 1986 Sep 1;189(5):564-5.
    pubmed: 2875986
  16. Bukar A, Mukhtar MD, Adam SA. Current trend in antimicrobial susceptibility pattern of Clostridium tetani isolated soil samples Kano. Bayero J. Pure Appl. Sci. 2008;1:112–115.
    doi: 10.4314/bajopas.v1i1.57539google scholar: lookup
  17. SANADA I, NISHIDA S. ISOLATION OF CLOSTRIDIUM TETANI FROM SOIL.. J Bacteriol 1965 Mar;89(3):626-9.
    pmc: PMC277512pubmed: 14275651doi: 10.1128/jb.89.3.626-629.1965google scholar: lookup
  18. Smith LD. Inhibition of Clostridium botulinum by strains of Clostridium perfringens isolated from soil.. Appl Microbiol 1975 Aug;30(2):319-23.
    pmc: PMC187173pubmed: 169734doi: 10.1128/am.30.2.319-323.1975google scholar: lookup
  19. Gohari IM, Arroyo L, Macinnes JI, Timoney JF, Parreira VR, Prescott JF. Characterization of Clostridium perfringens in the feces of adult horses and foals with acute enterocolitis.. Can J Vet Res 2014 Jan;78(1):1-7.
    pmc: PMC3878003pubmed: 24396174
  20. Murdoch DA. Gram-positive anaerobic cocci.. Clin Microbiol Rev 1998 Jan;11(1):81-120.
    doi: 10.1128/CMR.11.1.81pmc: PMC121377pubmed: 9457430google scholar: lookup
  21. Yoo HS, Lee SU, Park KY, Park YH. Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR.. J Clin Microbiol 1997 Jan;35(1):228-32.
    pmc: PMC229544pubmed: 8968913doi: 10.1128/jcm.35.1.228-232.1997google scholar: lookup
  22. Kalia VC, Mukherjee T, Bhushan A, Joshi J, Shankar P, Huma N. Analysis of the unexplored features of rrs (16S rDNA) of the Genus Clostridium.. BMC Genomics 2011 Jan 11;12:18.
    doi: 10.1186/1471-2164-12-18pmc: PMC3024285pubmed: 21223548google scholar: lookup
  23. Woo PC, Lau SK, Chan KM, Fung AM, Tang BS, Yuen KY. Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing.. J Clin Pathol 2005 Mar;58(3):301-7.
    doi: 10.1136/jcp.2004.022830pmc: PMC1770585pubmed: 15735165google scholar: lookup
  24. Fallani M, Rigottier-Gois L, Aguilera M, Bridonneau C, Collignon A, Edwards CA, Corthier G, Doré J. Clostridium difficile and Clostridium perfringens species detected in infant faecal microbiota using 16S rRNA targeted probes.. J Microbiol Methods 2006 Oct;67(1):150-61.
    doi: 10.1016/j.mimet.2006.03.010pubmed: 16647148google scholar: lookup
  25. Hwang JY, Cho GJ. First Identification of Taylorella equgenitalis From Genital Tracts of Thoroughbred Horses from the Inland Area of South Korea by Multilocus Sequence Typing. J. Equine Vet. Sci. 2018;60:16–22.
    doi: 10.1016/j.jevs.2017.09.011google scholar: lookup
  26. Farzan A, Kircanski J, DeLay J, Soltes G, Songer JG, Friendship R, Prescott JF. An investigation into the association between cpb2-encoding Clostridium perfringens type A and diarrhea in neonatal piglets.. Can J Vet Res 2013 Jan;77(1):45-53.
    pmc: PMC3525171pubmed: 23814355
  27. Andrews JM, Howe RA. BSAC standardized disc susceptibility testing method (version 10).. J Antimicrob Chemother 2011 Dec;66(12):2726-57.
    doi: 10.1093/jac/dkr359pubmed: 21921076google scholar: lookup
  28. Nachnani S, Scuteri A, Newman MG, Avanessian AB, Lomeli SL. E-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.. J Periodontol 1992 Jul;63(7):576-83.
    doi: 10.1902/jop.1992.63.7.576pubmed: 1324301google scholar: lookup
  29. Thornsberry C. NCCLS Standards for Antimicrobial Susceptibility Tests. Lab. Med. 2016;14:549–553.
    doi: 10.1093/labmed/14.9.549google scholar: lookup
  30. Feary DJ, Hassel DM. Enteritis and colitis in horses.. Vet Clin North Am Equine Pract 2006 Aug;22(2):437-79, ix.
    doi: 10.1016/j.cveq.2006.03.008pubmed: 16882483google scholar: lookup
  31. Lee KE, Lim SI, Shin SH, Kwon YK, Kim HY, Song JY, An DJ. Distribution of Clostridium perfringens isolates from piglets in South Korea.. J Vet Med Sci 2014 May;76(5):745-9.
    doi: 10.1292/jvms.13-0430pmc: PMC4073346pubmed: 24430655google scholar: lookup
  32. Lee YJ. Antimicrobial resistance and molecular characterization of Clostridium perfringens isolated from chicken. J. Prev. Vet. Med. 2016;40:71–79.
    doi: 10.13041/jpvm.2016.40.2.71google scholar: lookup
  33. Slavić D, Boerlin P, Fabri M, Klotins KC, Zoethout JK, Weir PE, Bateman D. Antimicrobial susceptibility of Clostridium perfringens isolates of bovine, chicken, porcine, and turkey origin from Ontario.. Can J Vet Res 2011 Apr;75(2):89-97.
    pmc: PMC3062930pubmed: 21731178

Citations

This article has been cited 4 times.
  1. Li J, Xie K, Yang J, Zhang J, Yang Q, Wang P, Gun S, Huang X. S100A9 plays a key role in Clostridium perfringens beta2 toxin-induced inflammatory damage in porcine IPEC-J2 intestinal epithelial cells. BMC Genomics 2023 Jan 12;24(1):16.
    doi: 10.1186/s12864-023-09118-6pubmed: 36635624google scholar: lookup
  2. Kerek Á, Szabó Á, Barnácz F, Csirmaz B, Kovács L, Jerzsele Á. Antimicrobial Susceptibility and Toxin Gene Profiles of Commensal Clostridium perfringens Isolates from Turkeys in Hungarian Poultry Farms (2022-2023). Antibiotics (Basel) 2025 Apr 17;14(4).
    doi: 10.3390/antibiotics14040413pubmed: 40298569google scholar: lookup
  3. Johnson S, Skinner AM, Lostutter C, Duke T, Posthaus H. Enteritis necroticans and Clostridium perfringens type C; Epidemiological and pathological findings over the past 20 years. PLoS Negl Trop Dis 2025 Feb;19(2):e0012836.
    doi: 10.1371/journal.pntd.0012836pubmed: 39908342google scholar: lookup
  4. Kabir A, Lamichhane B, Habib T, Adams A, El-Sheikh Ali H, Slovis NM, Troedsson MHT, Helmy YA. Antimicrobial Resistance in Equines: A Growing Threat to Horse Health and Beyond-A Comprehensive Review. Antibiotics (Basel) 2024 Jul 29;13(8).
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