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The Journal of veterinary medical science2016; 79(1); 206-212; doi: 10.1292/jvms.16-0506

The full genome sequences of 8 equine herpesvirus type 4 isolates from horses in Japan.

Abstract: Equine herpesvirus type 4 (EHV-4) is one of the most important pathogens in horses. To clarify the key genes of the EHV-4 genome that cause abortion in female horses, we determined the whole genome sequences of a laboratory strain and 7 Japanese EHV-4 isolates that were isolated from 2 aborted fetuses and nasal swabs of 5 horses with respiratory disease. The full genome sequences and predicted amino acid sequences of each gene of these isolates were compared with of the reference EHV-4 strain NS80567 and Australian isolates that were reported in 2015. The EHV-4 isolates clustered in 2 groups which did not reflect their pathogenicity. A comparison of the predicted amino acid sequences of the genes did not reveal any genes that were associated with EHV-4-induced abortion.
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Publication Date: 2016-11-14 PubMed ID: 27840393PubMed Central: PMC5289262DOI: 10.1292/jvms.16-0506Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses the full genome sequencing of eight equine herpesvirus type 4 isolates from horses in Japan, with the motivation to pinpoint key genes that could cause abortion in female horses. The findings, however, did not reveal any genes directly associated with the virus-induced abortion.

Research Methodology

  • At the core of the research is the study of equine herpesvirus type 4 (EHV-4), a major pathogen in horses.
  • Scientists carried out whole genome sequencing of a laboratory strain and seven Japanese EHV-4 isolates, which were collected from two aborted fetuses and nasal swabs of five horses with respiratory disease.

Data Comparison

  • The full genome sequences and predicted amino acid sequences of each gene of the isolated strains were compared against the reference strain of EHV-4 known as NS80567.
  • This data was also compared with Australian isolates that were documented in 2015.

Key Findings

  • An observation was made that the EHV-4 isolates fell into two groups, but these did not correspond to their pathogenicity or disease-causing capability.
  • Essentially, these groups were not classified based on the isolates’ ability to cause abortion in horses, ruling out the variables of their potential impact.
  • After comparing the predicted amino acid sequences of the genes, the study did not identify any genes directly linked with EHV-4-induced abortion in horses.

Conclusion

  • The key objective of the research was to uncover potential genes in the EHV-4 genome associated with abortion in female horses.
  • However, the comprehensive genome sequencing and subsequent comparison with reference strains did not reveal any direct associations, leading to a non-conclusive finding in this aspect.

Cite This Article

APA
Izume S, Kirisawa R, Ohya K, Ohnuma A, Kimura T, Omatsu T, Katayama Y, Mizutani T, Fukushi H. (2016). The full genome sequences of 8 equine herpesvirus type 4 isolates from horses in Japan. J Vet Med Sci, 79(1), 206-212. https://doi.org/10.1292/jvms.16-0506

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 79
Issue: 1
Pages: 206-212

Researcher Affiliations

Izume, Satoko
  • Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Kirisawa, Rikio
    Ohya, Kenji
      Ohnuma, Aiko
        Kimura, Takashi
          Omatsu, Tsutomu
            Katayama, Yukie
              Mizutani, Tetsuya
                Fukushi, Hideto

                  MeSH Terms

                  • Animals
                  • Female
                  • Genome, Viral / genetics
                  • Herpesviridae Infections / epidemiology
                  • Herpesviridae Infections / veterinary
                  • Herpesviridae Infections / virology
                  • Herpesvirus 4, Equid / genetics
                  • Herpesvirus 4, Equid / isolation & purification
                  • Horse Diseases / epidemiology
                  • Horse Diseases / virology
                  • Horses / virology
                  • Japan / epidemiology
                  • Sequence Analysis, DNA / veterinary

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                  Citations

                  This article has been cited 7 times.
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                  3. Câmara RJF, Bueno BL, Resende CF, Balasuriya UBR, Sakamoto SM, Reis JKPD. Viral Diseases that Affect Donkeys and Mules. Animals (Basel) 2020 Nov 25;10(12).
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                  4. Kolb AW, Lewin AC, Moeller Trane R, McLellan GJ, Brandt CR. Phylogenetic and recombination analysis of the herpesvirus genus varicellovirus. BMC Genomics 2017 Nov 21;18(1):887.
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