The influence of repeated arthrocentesis and exercise on matrix metalloproteinase and tumour necrosis factor alpha activities in normal equine joints.
Abstract: Matrix metalloproteinases (MMPs) and tumour necrosis factor alpha (TNF-alpha) may be useful as biomarkers of joint disease or inflammation. However, activity of both MMPs and TNF-alpha in synovial fluid (SF) may be influenced by nonpathological factors such as arthrocentesis or exercise. Objective: To investigate the influence of repeated arthrocentesis and exercise on MMP and TNF-alpha activities in SF from normal equine joints. Methods: SF was collected from the left metacarpophalangeal, radiocarpal and tarsocrural joints of 16 horses. Eight of these horses were subsequently subjected to an exercise programme on a treadmill and 8 were box-rested as controls. Arthrocentesis was repeated 14, 145, 17 and 24 days after the start of the exercise programme. General MMP and TNF-alpha activities were determined in SF. Results: Repeated arthrocentesis caused a gradual increase but the exercise regimen no significant increase in MMP activity. There was a significant increase in TNF-alpha activity in SF collected from horses 2 h after cessation of the exercise programme. Conclusions: When using MMPs as biomarkers for joint disease, at least 14 days should elapse after previous arthrocentesis before subsequent SF collection. Moderate exercise does not increase MMP activity in SF from normal joints and it may be possible to ignore this as a source of error in evaluating MMP activity in diseased joints.
Publication Date: 2004-03-25 PubMed ID: 15038439DOI: 10.2746/0425164044868602Google Scholar: Lookup
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- Journal Article
Summary
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The research article investigates the impact of repeated arthrocentesis and exercise on matrix metalloproteinase and tumour necrosis factor alpha in normal equine joints, highlighting their potential relevance as biomarkers of joint disease or inflammation.
Research Objectives and Method
- The main goal of the study was to analyze the effect of regular arthrocentesis and physical activity on the activities of matrix metalloproteinases (MMPs) and tumour necrosis factor alpha (TNF-alpha) in the synovial fluid (SF) of normal equine joints.
- The researchers collected SF from various joints of 16 horses. Of these, 8 horses underwent an exercise programme on a treadmill while the remaining 8 were kept as control (box-rested).
- The process of arthrocentesis was repeated on the 14th, 17th, and 24th days after the beginning of the exercise programme. The general MMP and TNF-alpha activities were determined in the SF.
Results of the Research
- The research found that there was a gradual increase in MMP activity due to repeated arthrocentesis, but no significant change due to the exercise regimen.
- The study also observed a significant increase in TNF-alpha activity in the SF gathered from horses 2 hours after the conclusion of the exercise program.
Conclusions and Implications
- From the research, it was concluded that when utilizing MMPs as biomarkers for joint disease, a minimum of 14 days should pass after previous arthrocentesis before the next SF collection.
- Furthermore, moderate exercise does not lead to an increase in MMP activity in the SF from normal joints, indicating it can potentially be disregarded as an error source when assessing MMP activity in diseased joints.
This study provides crucial insights into the potential influence of non-pathological factors such as arthrocentesis and exercise on biomarkers of joint disease, contributing valuable information to the field of veterinary joint disease diagnostics and management.
Cite This Article
APA
van den Boom R, Brama PA, Kiers GH, DeGroot J, Barneveld A, van Weeren RR.
(2004).
The influence of repeated arthrocentesis and exercise on matrix metalloproteinase and tumour necrosis factor alpha activities in normal equine joints.
Equine Vet J, 36(2), 155-159.
https://doi.org/10.2746/0425164044868602 Publication
Researcher Affiliations
- Department of Equine Sciences, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 12, Utrecht, The Netherlands.
MeSH Terms
- Animals
- Biological Assay / methods
- Biological Assay / veterinary
- Biomarkers / analysis
- Cell Line, Tumor
- Female
- Horse Diseases / diagnosis
- Horse Diseases / enzymology
- Horses
- Joint Diseases / diagnosis
- Joint Diseases / enzymology
- Joint Diseases / veterinary
- Joints / enzymology
- Joints / metabolism
- Male
- Matrix Metalloproteinases / metabolism
- Paracentesis / adverse effects
- Paracentesis / veterinary
- Physical Conditioning, Animal / physiology
- Random Allocation
- Synovial Fluid / enzymology
- Synovial Fluid / metabolism
- Tumor Necrosis Factor-alpha / metabolism
Citations
This article has been cited 13 times.- Andersen C, Walters M, Bundgaard L, Berg LC, Vonk LA, Lundgren-Åkerlund E, Henriksen BL, Lindegaard C, Skovgaard K, Jacobsen S. Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses. Front Vet Sci 2024;11:1374681.
- Vishwanath K, Secor EJ, Watkins A, Reesink HL, Bonassar LJ. Loss of effective lubricating viscosity is the primary mechanical marker of joint inflammation in equine synovitis. J Orthop Res 2024 Jul;42(7):1438-1447.
- Kearney CM, Korthagen NM, Plomp SGM, Labberté MC, de Grauw JC, van Weeren PR, Brama PAJ. A Translational Model for Repeated Episodes of Joint Inflammation: Welfare, Clinical and Synovial Fluid Biomarker Assessment. Animals (Basel) 2023 Oct 12;13(20).
- Kearney CM, Khatab S, van Buul GM, Plomp SGM, Korthagen NM, Labberté MC, Goodrich LR, Kisiday JD, Van Weeren PR, van Osch GJVM, Brama PAJ. Treatment Effects of Intra-Articular Allogenic Mesenchymal Stem Cell Secretome in an Equine Model of Joint Inflammation. Front Vet Sci 2022;9:907616.
- Mohammadi A, Te Moller NCR, Ebrahimi M, Plomp S, Brommer H, van Weeren PR, Mäkelä JTA, Töyräs J, Korhonen RK. Site- and Zone-Dependent Changes in Proteoglycan Content and Biomechanical Properties of Bluntly and Sharply Grooved Equine Articular Cartilage. Ann Biomed Eng 2022 Dec;50(12):1787-1797.
- Watkins A, Fasanello D, Stefanovski D, Schurer S, Caracappa K, D'Agostino A, Costello E, Freer H, Rollins A, Read C, Su J, Colville M, Paszek M, Wagner B, Reesink H. Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models. BMC Vet Res 2021 May 12;17(1):189.
- Te Moller NCR, Mohammadi A, Plomp S, Serra Bragança FM, Beukers M, Pouran B, Afara IO, Nippolainen E, Mäkelä JTA, Korhonen RK, Töyräs J, Brommer H, van Weeren PR. Structural, compositional, and functional effects of blunt and sharp cartilage damage on the joint: A 9-month equine groove model study. J Orthop Res 2021 Nov;39(11):2363-2375.
- Niemelä TM, Tulamo RM, Carmona JU, López C. Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses. Acta Vet Scand 2019 May 30;61(1):24.
- Andreassen SM, Vinther AML, Nielsen SS, Andersen PH, Tnibar A, Kristensen AT, Jacobsen S. Changes in concentrations of haemostatic and inflammatory biomarkers in synovial fluid after intra-articular injection of lipopolysaccharide in horses. BMC Vet Res 2017 Jun 19;13(1):182.
- Moraes AP, Moreira JJ, Brossi PM, Machado TS, Michelacci YM, Baccarin RY. Short- and long-term effects of platelet-rich plasma upon healthy equine joints: Clinical and laboratory aspects. Can Vet J 2015 Aug;56(8):831-8.
- Moreira JJ, Moraes AP, Brossi PM, Machado TS, Michelacci YM, Massoco CO, Baccarin RY. Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints. J Vet Sci 2015;16(1):47-55.
- Lamprecht ED, Williams CA. Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses. Oxid Med Cell Longev 2012;2012:920932.
- Francoz D, Desrochers A, Latouche JS. Effect of repeated arthrocentesis and single joint lavage on cytologic evaluation of synovial fluid in 5 young calves. Can J Vet Res 2007 Apr;71(2):129-34.
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