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Home / Equine Research Bank / Vet Res / The innate immune response of equine bronchial epithelial ce...
Veterinary research2015; 46(1); 3; doi: 10.1186/s13567-014-0126-3

The innate immune response of equine bronchial epithelial cells is altered by training.

Abstract: Respiratory diseases, including inflammatory airway disease (IAD), viral and bacterial infections, are common problems in exercising horses. The airway epithelium constitutes a major physical barrier against airborne infections and plays an essential role in the lung innate immune response mainly through toll-like receptor (TLR) activation. The aim of this study was to develop a model for the culture of equine bronchial epithelial cells (EBEC) in vitro and to explore EBEC innate immune responses in trained horses. Bronchial epithelial biopsies were taken from 6 adult horses during lower airway endoscopy. EBEC were grown in vitro by an explant method. The innate immune response of EBEC was evaluated in vitro by treatment with TLR ligands. TLR3 is the most strongly expressed TLR at the mRNA level in EBEC and stimulation of EBEC with Poly(I:C), an analog of viral dsRNA, triggers a strong secretion of IFN-β, TNF-α, IL-6 and CXCL8. We further evaluated the EBEC innate immune response in horses that underwent a 4-month-training program. While training had no effect on TLR mRNA expression in EBEC as well as in bronchial biopsies, it increased the production of IFN-β after stimulation with a TLR3 ligand and decreased the secretion of TNF-α and IL-6 after stimulation with a TLR2 and TLR3 ligand. These findings may be implicated in the increased risk for viral and bacterial infections observed in sport horses. Altogether, we report a successful model for the culture of EBEC that can be applied to the investigation of pathophysiologic conditions in longitudinal studies.
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Publication Date: 2015-01-17 PubMed ID: 25595212PubMed Central: PMC4297379DOI: 10.1186/s13567-014-0126-3Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study focuses on the effect of training on the innate immune response in equine bronchial epithelial cells. It found that training altered the immune response, possibly increasing the risk of viral and bacterial infections in sport horses.

Background

  • This research is centered on understanding respiratory diseases in horses, particularly those involved in heavy exercises. Conditions such as inflammatory airway diseases, as well as viral and bacterial infections, are often found in such horses.
  • The study highlights the role of the airway epithelium as the primary physical barrier against airborne infections, with its function greatly tied to the activation of toll-like receptors (TLR) as part of the lung’s innate immune response.
  • The primary aim of the research was to establish an in vitro culture model for equine bronchial epithelial cells (EBEC) and to explore how these cells’ innate immune responses are affected in trained horses.

Methods

  • Equestrian bronchial epithelial biopsies were obtained from six adult horses through lower airway endoscopy.
  • The biopsied cells were then grown in vitro using an explant method.
  • The innate immune response of the EBEC was assessed by treating these cells with TLR ligands.
  • According to the findings, TLR3 is the TLR most distinctly expressed at the mRNA level in EBEC. Stimulation of EBEC with Poly(I:C), a simulated version of viral dsRNA, triggered a strong release of IFN-β, TNF-α, IL-6, and CXCL8.

Findings

  • The second part of the study involved evaluating the innate immune response of EBEC in horses that underwent a four-month training program.
  • Training had no effect on TLR mRNA expression either in EBEC or bronchial biopsies, but it did significantly increase the production of IFN-β after stimulation with a TLR3 ligand.
  • Moreover, training led to a reduction in the secretion of TNF-α and IL-6, following stimulation with TLR2 and TLR3 ligands.
  • These alterations in immune responses could potentially lead to an increased susceptibility to viral and bacterial infections in sport horses.

Conclusion

  • The research provides a successful model for cultivating EBEC in vitro, which can be a valuable tool for future investigations into pathophysiological conditions in longitudinal studies.

Cite This Article

APA
Frellstedt L, Gosset P, Kervoaze G, Hans A, Desmet C, Pirottin D, Bureau F, Lekeux P, Art T. (2015). The innate immune response of equine bronchial epithelial cells is altered by training. Vet Res, 46(1), 3. https://doi.org/10.1186/s13567-014-0126-3

Publication

Veterinary research
ISSN: 1297-9716
NlmUniqueID: 9309551
Country: England
Language: English
Volume: 46
Issue: 1
Pages: 3

Researcher Affiliations

Frellstedt, Linda
  • Center of Equine Sports Medicine, University of Liege, Liege, Belgium. linda.frellstedt@ulg.ac.be.
Gosset, Philippe
    Kervoaze, Gwenola
      Hans, Aymeric
        Desmet, Christophe
          Pirottin, Dimitri
            Bureau, Fabrice
              Lekeux, Pierre
                Art, Tatiana

                  MeSH Terms

                  • Animals
                  • Bronchi / cytology
                  • Bronchi / immunology
                  • Cell Culture Techniques / methods
                  • Cells, Cultured
                  • Cytokines / metabolism
                  • Female
                  • Horses / genetics
                  • Horses / immunology
                  • Horses / physiology
                  • Immunity, Innate
                  • Male
                  • Physical Conditioning, Animal
                  • Poly I-C / pharmacology
                  • Respiratory Mucosa / cytology
                  • Respiratory Mucosa / immunology
                  • Toll-Like Receptors / genetics
                  • Toll-Like Receptors / metabolism

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                  Citations

                  This article has been cited 8 times.
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