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Home / Equine Research Bank / Can J Vet Res / The isoelectric focusing properties of serum alkaline phosph...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire1990; 54(1); 126-131;

The isoelectric focusing properties of serum alkaline phosphatase in disease and following prednisolone and phenylbutazone administration in the horse.

Abstract: This study was undertaken to ascertain if the isoelectric focusing pattern of serum alkaline phosphatase (AP) from sick horses with high activity is useful for determining its tissue origin. The effect of oral prednisolone and phenylbutazone therapy on this enzyme in healthy horses was also investigated. The sick horses were divided into three groups: hepatic, intestinal and miscellaneous. All sera had approximately thirteen bands of AP activity when focused on agarose gels with a pH gradient of 3.5 to 9.5. All the horses in the liver disease group had greater than 65% of enzyme activity in bands 3 to 7 (counted from the anode) whereas the other two groups had at least 30% and up to 80% of activity in bands 8 to 13. This was true even in the several cases of primary intestinal disease that had additional biochemical evidence of liver damage. All bands were heat sensitive indicating that little if any AP was of small intestinal or renal origin. Oral prednisolone and phenylbutazone for 20 and 12 days respectively had no affect on serum AP activity or isoelectric pattern. We concluded that the AP in bands 3 to 7 is of liver origin but the origin of bands 8 to 13 remains undetermined although small intestinal or renal origin is unlikely. Isoelectric focusing of serum AP shows promise in differentiating cases of primary from secondary liver disease but further studies are required correlating serum patterns and tissue patterns in animals with diseases.
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Publication Date: 1990-01-01 PubMed ID: 2306661PubMed Central: PMC1255616
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines how the isoelectric focusing pattern of a specific enzyme, serum alkaline phosphatase, in ill horses can help determine its tissue source. It also looks at how medications like prednisolone and phenylbutazone affect this enzyme.

Objective of Research

  • The main purpose of this study was to determine if the isoelectric focusing pattern of the enzyme serum alkaline phosphatase (AP) from sick horses can help identify its tissue of origin.
  • Additionally, the investigation sought to understand the impact of medications, specifically oral prednisolone and phenylbutazone, on this enzyme in healthy horses.

Methodology

  • Sick horses were categorized into three groups: hepatic (liver disease), intestinal, and miscellaneous.
  • All serum samples from the horses exhibited around thirteen bands of AP activity when focused on agarose gels with a pH gradient of 3.5 to 9.5.
  • The researchers controlled for variables by testing the predator and phenylbutazone on healthy horses for 20 and 12 days consecutively.

Findings

  • In the group of horses with liver disease, over 65% of enzyme activity was noted in bands 3 to 7.
  • In the other two groups, between 30% and 80% of activity was observed in bands 8 to 13.
  • Despite some cases of intestinal disease also having biochemical markers for liver damage, the band pattern was consistent.
  • All bands were heat sensitive, suggesting that the origin of AP was likely not the small intestine or kidneys.
  • The administration of prednisolone and phenylbutazone was found to have no impact on serum AP activity or isoelectric pattern.

Conclusions

  • The enzyme AP in bands 3 to 7 is believed to have a liver origin.
  • The source of AP in bands 8 to 13 is still undecided, although it is unlikely to be from the small intestine or kidneys.
  • Isoelectric focusing of serum AP demonstrates potential in differentiating primary from secondary liver disease, although more research is needed to correlate serum patterns and tissue patterns in animals with diseases.

Cite This Article

APA
Ellison RS, Jacobs RM. (1990). The isoelectric focusing properties of serum alkaline phosphatase in disease and following prednisolone and phenylbutazone administration in the horse. Can J Vet Res, 54(1), 126-131.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 54
Issue: 1
Pages: 126-131

Researcher Affiliations

Ellison, R S
  • Department of Pathology, Ontario Veterinary College, University of Guelph.
Jacobs, R M

    MeSH Terms

    • Alkaline Phosphatase / blood
    • Animals
    • Female
    • Horse Diseases / blood
    • Horse Diseases / enzymology
    • Horses
    • Intestinal Diseases / blood
    • Intestinal Diseases / enzymology
    • Intestinal Diseases / veterinary
    • Isoelectric Focusing
    • Liver Diseases / blood
    • Liver Diseases / enzymology
    • Liver Diseases / veterinary
    • Male
    • Phenylbutazone / pharmacology
    • Prednisolone / pharmacology

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