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Canadian journal of comparative medicine : Revue canadienne de medecine comparee1974; 38(3); 303-313;

The kinetics of hematopoiesis in the light horse. I. The lifespan of peripheral blood cells in the normal horse.

Abstract: Three Standardbred horses were given 0.2 mg (1 mCi) of (75)selenomethionine intravenously and a second group of three were given 10 mCi of tritiated diisopropylfluorophosphate (0.5 mg) intravenously. Observations on labeled cells were continued for 250 days after radioselenium injection and 160 days after tritium injection. The lifespan of erythrocytes using (75)selenmethionine was 155 +/- 10 days and 148 +/- 7.8 days using tritiated diisopropylfluorophosphate. There was no significant difference at the 10% level between the lifespans, using these labels. The uptake of radioselenium into erythrocytes reached a mean maximum of 11.5% at 82 +/- 18.9 days after injection of the label. There was an elution of from 17.6% of the injected dose of tritium label down to 7.5% eight days after injection of this isotope. From this study both of these labels appear to be satisfactory for determining the erythrocyte lifespan of the horse. The mean time of the curve of mean radioselenium activity of peripheral blood leukocytes for the three horses was 5.54 days and the lifespan of these cells was seven days. The mean lifespan of peripheral blood leukocytes of the three horses after in vivo labelling with tritiated diisopropylfluorophosphate was 17.3 hours. No specific labelling was found in bone marrow or peripheral blood cells at this level of tritium labelling by dipping emulsion autoradiography. The mean lifespan of equine platelets for three horses using radioselenomethionine was 9.2 days and the mean lifespan of equine platelets using tritiated diisopropylfluorophosphate was 6.6 days in a group of three horses.
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Publication Date: 1974-07-01 PubMed ID: 4277706PubMed Central: PMC1319873
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the lifespan of different blood cells in normal horses, using radioactive substances for tracing. The study found that the average lifespan for erythrocytes is about 155 days, leukocytes live for about seven days, and platelets live for around 9.2 days.

Methods

  • Two groups of three Standardbred horses each were injected with different radioactive substances to trace the lifespan of blood cells.
  • The first group was given 0.2 mg (1 mCi) of selenomethionine-75 intravenously, while the second group was given 10 mCi of tritiated diisopropylfluorophosphate (0.5 mg) intravenously.

Results

  • The lifespan of erythrocytes was estimated to be 155 days and 148 days, respectively, using selenomethionine-75 and tritiated diisopropylfluorophosphate. The results showed no significant difference at the 10% level between the lifespans, suggesting that both these labels were effectively used to determine the erythrocyte lifespan.
  • The uptake of radioselenium into erythrocytes reached a mean maximum of 11.5% at 82 days after injection. It was noted that there was an elution from 17.6% of the injected dose of tritium label down to 7.5% eight days after injection.
  • The average lifespan of peripheral blood leukocytes for the three horses was seven days, with the mean time of the curve for radioselenium activity being 5.54 days. For in vivo labelling with tritiated diisopropylfluorophosphate, this lifespan was found to be 17.3 hours.
  • No specific labelling was observed in the bone marrow or peripheral blood cells at the level of tritium labelling provided. This suggests that this method may not be effective for these types of cells.
  • Using selenomethionine-75, the mean lifespan of equine platelets for three horses was found to be 9.2 days, while the mean lifespan of platelets using tritiated diisopropylfluorophosphate was 6.6 days in a group of three horses.

Conclusion

  • The research succeeded in estimating the lifespans of erythrocytes, leukocytes and platelets in horses. This information can be crucial for understanding horse health and diagnosing blood-related diseases.

Cite This Article

APA
Carter EI, Valli VE, McSherry BJ, Milne FJ, Robinson GA, Lumsden JH. (1974). The kinetics of hematopoiesis in the light horse. I. The lifespan of peripheral blood cells in the normal horse. Can J Comp Med, 38(3), 303-313.

Publication

Canadian journal of comparative medicine : Revue canadienne de medecine comparee
ISSN: 0008-4050
NlmUniqueID: 0151747
Country: Canada
Language: English
Volume: 38
Issue: 3
Pages: 303-313

Researcher Affiliations

Carter, E I
    Valli, V E
      McSherry, B J
        Milne, F J
          Robinson, G A
            Lumsden, J H

              MeSH Terms

              • Animals
              • Autoradiography
              • Blood Cells / physiology
              • Blood Platelets / physiology
              • Bone Marrow Cells
              • Erythrocytes / physiology
              • Hematocrit
              • Hematopoiesis
              • Hemoglobins / analysis
              • Horses / physiology
              • Injections, Intravenous
              • Isoflurophate / administration & dosage
              • Isotope Labeling
              • Leukocytes / physiology
              • Methionine / administration & dosage
              • Radioisotopes
              • Selenium / administration & dosage
              • Time Factors
              • Tritium

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              Citations

              This article has been cited 6 times.
              1. Satué K, Fazio E, La Fauci D, Medica P. Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares. Animals (Basel) 2023 Mar 31;13(7).
                doi: 10.3390/ani13071229pubmed: 37048485google scholar: lookup
              2. Thorn B, Dunstan RH, Macdonald MM, Borges N, Roberts TK. Evidence that human and equine erythrocytes could have significant roles in the transport and delivery of amino acids to organs and tissues. Amino Acids 2020 May;52(5):711-724.
                doi: 10.1007/s00726-020-02845-0pubmed: 32318874google scholar: lookup
              3. Kämpf S, Seiler E, Bujok J, Hofmann-Lehmann R, Riond B, Makhro A, Bogdanova A. Aging Markers in Equine Red Blood Cells. Front Physiol 2019;10:893.
                doi: 10.3389/fphys.2019.00893pubmed: 31379601google scholar: lookup
              4. Lumsden HJ, Valli VE, McSherry BJ, Robinson GA, Claxton MJ. The kinetics of hematopoiesis in the light horse III. The hematological response to hemolytic anemia. Can J Comp Med 1975 Jul;39(3):332-9.
                pubmed: 1139414
              5. Lumsden JH, Valli VE, McSherry BJ, Robinson GA, Claxton MJ. The kinetics of hematopoiesis in the light horse II. The hematological response to hemorrhagic anemia. Can J Comp Med 1975 Jul;39(3):324-31.
                pubmed: 1139413
              6. Baird JD, Arroyo LG, Lumsden JH. Whole blood glutathione peroxidase activity in Standardbred broodmares supplemented with vitamin E and selenium. Can J Vet Res 2026 Jan;90(1):25-29.
                pubmed: 41585003
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