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Home / Equine Research Bank / Can J Comp Med / The kinetics of hematopoiesis in the light horse. I. The lif...
Canadian journal of comparative medicine : Revue canadienne de medecine comparee1974; 38(3); 303-313;

The kinetics of hematopoiesis in the light horse. I. The lifespan of peripheral blood cells in the normal horse.

Abstract: Three Standardbred horses were given 0.2 mg (1 mCi) of (75)selenomethionine intravenously and a second group of three were given 10 mCi of tritiated diisopropylfluorophosphate (0.5 mg) intravenously. Observations on labeled cells were continued for 250 days after radioselenium injection and 160 days after tritium injection. The lifespan of erythrocytes using (75)selenmethionine was 155 +/- 10 days and 148 +/- 7.8 days using tritiated diisopropylfluorophosphate. There was no significant difference at the 10% level between the lifespans, using these labels. The uptake of radioselenium into erythrocytes reached a mean maximum of 11.5% at 82 +/- 18.9 days after injection of the label. There was an elution of from 17.6% of the injected dose of tritium label down to 7.5% eight days after injection of this isotope. From this study both of these labels appear to be satisfactory for determining the erythrocyte lifespan of the horse. The mean time of the curve of mean radioselenium activity of peripheral blood leukocytes for the three horses was 5.54 days and the lifespan of these cells was seven days. The mean lifespan of peripheral blood leukocytes of the three horses after in vivo labelling with tritiated diisopropylfluorophosphate was 17.3 hours. No specific labelling was found in bone marrow or peripheral blood cells at this level of tritium labelling by dipping emulsion autoradiography. The mean lifespan of equine platelets for three horses using radioselenomethionine was 9.2 days and the mean lifespan of equine platelets using tritiated diisopropylfluorophosphate was 6.6 days in a group of three horses.
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Publication Date: 1974-07-01 PubMed ID: 4277706PubMed Central: PMC1319873
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the lifespan of different blood cells in normal horses, using radioactive substances for tracing. The study found that the average lifespan for erythrocytes is about 155 days, leukocytes live for about seven days, and platelets live for around 9.2 days.

Methods

  • Two groups of three Standardbred horses each were injected with different radioactive substances to trace the lifespan of blood cells.
  • The first group was given 0.2 mg (1 mCi) of selenomethionine-75 intravenously, while the second group was given 10 mCi of tritiated diisopropylfluorophosphate (0.5 mg) intravenously.

Results

  • The lifespan of erythrocytes was estimated to be 155 days and 148 days, respectively, using selenomethionine-75 and tritiated diisopropylfluorophosphate. The results showed no significant difference at the 10% level between the lifespans, suggesting that both these labels were effectively used to determine the erythrocyte lifespan.
  • The uptake of radioselenium into erythrocytes reached a mean maximum of 11.5% at 82 days after injection. It was noted that there was an elution from 17.6% of the injected dose of tritium label down to 7.5% eight days after injection.
  • The average lifespan of peripheral blood leukocytes for the three horses was seven days, with the mean time of the curve for radioselenium activity being 5.54 days. For in vivo labelling with tritiated diisopropylfluorophosphate, this lifespan was found to be 17.3 hours.
  • No specific labelling was observed in the bone marrow or peripheral blood cells at the level of tritium labelling provided. This suggests that this method may not be effective for these types of cells.
  • Using selenomethionine-75, the mean lifespan of equine platelets for three horses was found to be 9.2 days, while the mean lifespan of platelets using tritiated diisopropylfluorophosphate was 6.6 days in a group of three horses.

Conclusion

  • The research succeeded in estimating the lifespans of erythrocytes, leukocytes and platelets in horses. This information can be crucial for understanding horse health and diagnosing blood-related diseases.

Cite This Article

APA
Carter EI, Valli VE, McSherry BJ, Milne FJ, Robinson GA, Lumsden JH. (1974). The kinetics of hematopoiesis in the light horse. I. The lifespan of peripheral blood cells in the normal horse. Can J Comp Med, 38(3), 303-313.

Publication

Canadian journal of comparative medicine : Revue canadienne de medecine comparee
ISSN: 0008-4050
NlmUniqueID: 0151747
Country: Canada
Language: English
Volume: 38
Issue: 3
Pages: 303-313

Researcher Affiliations

Carter, E I
    Valli, V E
      McSherry, B J
        Milne, F J
          Robinson, G A
            Lumsden, J H

              MeSH Terms

              • Animals
              • Autoradiography
              • Blood Cells / physiology
              • Blood Platelets / physiology
              • Bone Marrow Cells
              • Erythrocytes / physiology
              • Hematocrit
              • Hematopoiesis
              • Hemoglobins / analysis
              • Horses / physiology
              • Injections, Intravenous
              • Isoflurophate / administration & dosage
              • Isotope Labeling
              • Leukocytes / physiology
              • Methionine / administration & dosage
              • Radioisotopes
              • Selenium / administration & dosage
              • Time Factors
              • Tritium

              References

              This article includes 24 references
              1. Najean Y, Ardaillou N. The use of 75Se-methionin for the in vivo study of platelet kinetics.. Scand J Haematol 1969;6(6):395-401.
                pubmed: 5372959doi: 10.1111/j.1600-0609.1969.tb01833.xgoogle scholar: lookup
              2. Valli VE, Hulland TJ, McSherry BJ, Robinson GA, Gilman JP. The kinetics of haematopoiesis in the calf. I. An autoradiographical study of myelopoiesis in normal, anaemic and endotoxin treated calves.. Res Vet Sci 1971 Nov;12(6):535-50.
                pubmed: 5145350
              3. VAN PUTTEN LM. The life span of red cells in the rat and the mouse as determined by labeling with DFP32 in vivo.. Blood 1958 Aug;13(8):789-94.
                pubmed: 13560578
              4. BOVE JR, EBAUGH FG Jr. The use of diisopropylfluorophosphate 32 for the determination of in-vivo red cell survival and plasma cholinesterase turnover rates.. J Lab Clin Med 1958 Jun;51(6):916-25.
                pubmed: 13549833
              5. MARCILESE NA, VALSECCHI RM, FIGUEIRAS HD, CAMBEROS HR, VARELA JE. NORMAL BLOOD VOLUMES IN THE HORSE.. Am J Physiol 1964 Jul;207:223-7.
                pubmed: 14193594doi: 10.1152/ajplegacy.1964.207.1.223google scholar: lookup
              6. Wu AM, Till JE, Siminovitch L, McCulloch EA. Cytological evidence for a relationship between normal hemotopoietic colony-forming cells and cells of the lymphoid system.. J Exp Med 1968 Mar 1;127(3):455-64.
                pubmed: 5636553doi: 10.1084/jem.127.3.455google scholar: lookup
              7. McIntyre PA, Evatt B, Hodkinson BA, Scheffel U. Selenium-75 seleno-methionine as a label for erythrocytes, leukocytes, and platelets in man.. J Lab Clin Med 1970 Mar;75(3):472-80.
                pubmed: 5416903
              8. Evatt BL, Levin J. Measurement of thrombopoiesis in rabbits using 75selenomethionine.. J Clin Invest 1969 Sep;48(9):1615-26.
                pubmed: 5822574doi: 10.1172/JCI106127google scholar: lookup
              9. Sears DA, Weed RI. Diisopropylfluorophosphate is not a specific label for the red cell membrane.. Blood 1969 Sep;34(3):376-9.
                pubmed: 5804026
              10. NEUBERGER A, NIVEN JS. Haemoglobin formation in rabbits.. J Physiol 1951 Feb;112(3-4):292-310.
                pubmed: 14825213doi: 10.1113/jphysiol.1951.sp004530google scholar: lookup
              11. Perry S, Godwin HA, Zimmerman TS. Physiology of the granulocyte. 2.. JAMA 1968 Mar 18;203(12):1025-32.
                pubmed: 4868592doi: 10.1001/jama.203.12.1025google scholar: lookup
              12. Marcilese NA, Figueiras HD, Kremenchuzky S, Valsecchi RM, Camberos HR, Varela JE. Red cell survival time in the horse, determined with di-isopropyl-phosphorofluoridate-P.. Am J Physiol 1966 Aug;211(2):281-2.
                pubmed: 5921090doi: 10.1152/ajplegacy.1966.211.2.281google scholar: lookup
              13. Marcilese NA, Figueiras HD, Valsecchi RM, Fraga AH, Camberos HR, Varela JE. Erythrokinetics in the horse.. Am J Physiol 1965 Oct;209(4):727-30.
                pubmed: 5843425doi: 10.1152/ajplegacy.1965.209.4.727google scholar: lookup
              14. CARTWRIGHT GE, ATHENS JW, WINTROBE MM. THE KINETICS OF GRANULOPOIESIS IN NORMAL MAN.. Blood 1964 Dec;24:780-803.
                pubmed: 14235362
              15. Yoshida Y, Todo A, Shirakawa S, Wakisaka G, Uchino H. Proliferation of megaloblasts in pernicious anemia as observed from nucleic acid metabolism.. Blood 1968 Mar;31(3):292-303.
                pubmed: 5640627
              16. Brodsky I, Siegel NH, Kahn SB, Ross EM, Petkov G. Simultaneous fibrinogen and platelet survival with (75Se)selenomethionine in man. Studies in diseases with normal coagulation and in hepatocellular disease with abnormal coagulation.. Br J Haematol 1970 Mar;18(3):341-55.
                pubmed: 5491585doi: 10.1111/j.1365-2141.1970.tb01448.xgoogle scholar: lookup
              17. Cheng HF, Peterson RE, Evans TC. Incorporation of 75Se-selenomethionine in human peripheral lymphocytes as an in vitro test of globulin-synthesizing capacity.. J Nucl Med 1969 Feb;10(2):63-7.
                pubmed: 5784708
              18. HJORT PF, PAPUTCHIS H, CHENEY B. Labeling of red blood cells with radioactive diisopropylfluorophosphate (DFP32): evidence for an initial release of label.. J Lab Clin Med 1960 Mar;55:416-24.
                pubmed: 14402075
              19. Ebbe S, Stohlman F Jr, Donovan J, Howard D. Platelet survival in the rat as measured with tritium-labeled diisopropylfluorophosphate.. J Lab Clin Med 1966 Aug;68(2):233-43.
                pubmed: 5912598
              20. CLINE MJ, BERLIN NI. Measurement of red cell survival with tritiated diisopropylfluorophosphate.. J Lab Clin Med 1962 Nov;60:826-32.
                pubmed: 14021761
              21. McGuire TC, Henson JB, Quist SE. Viral-induced hemolysis in equine infectious anemia.. Am J Vet Res 1969 Dec;30(12):2091-7.
                pubmed: 5389415
              22. Carter EI, Valli VE, McSherry BJ. The separation of peripheral blood cells of the horse.. Can J Comp Med 1974 Jan;38(1):72-4.
                pubmed: 4272960
              23. Maloney MA, Patt HM. Granulocyte transit from bone marrow to blood.. Blood 1968 Feb;31(2):195-201.
                pubmed: 5643707
              24. Penner JA. Investigation of erythrocyte turnover with selenium-75-labeled methionine.. J Lab Clin Med 1966 Mar;67(3):427-38.
                pubmed: 5910150

              Citations

              This article has been cited 5 times.
              1. Satué K, Fazio E, La Fauci D, Medica P. Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares.. Animals (Basel) 2023 Mar 31;13(7).
                doi: 10.3390/ani13071229pubmed: 37048485google scholar: lookup
              2. Thorn B, Dunstan RH, Macdonald MM, Borges N, Roberts TK. Evidence that human and equine erythrocytes could have significant roles in the transport and delivery of amino acids to organs and tissues.. Amino Acids 2020 May;52(5):711-724.
                doi: 10.1007/s00726-020-02845-0pubmed: 32318874google scholar: lookup
              3. Kämpf S, Seiler E, Bujok J, Hofmann-Lehmann R, Riond B, Makhro A, Bogdanova A. Aging Markers in Equine Red Blood Cells.. Front Physiol 2019;10:893.
                doi: 10.3389/fphys.2019.00893pubmed: 31379601google scholar: lookup
              4. Lumsden HJ, Valli VE, McSherry BJ, Robinson GA, Claxton MJ. The kinetics of hematopoiesis in the light horse III. The hematological response to hemolytic anemia.. Can J Comp Med 1975 Jul;39(3):332-9.
                pubmed: 1139414
              5. Lumsden JH, Valli VE, McSherry BJ, Robinson GA, Claxton MJ. The kinetics of hematopoiesis in the light horse II. The hematological response to hemorrhagic anemia.. Can J Comp Med 1975 Jul;39(3):324-31.
                pubmed: 1139413
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