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Home / Equine Research Bank / Arch Virol / The nucleotide sequence of asinine herpesvirus 3 glycoprotei...
Archives of virology1995; 140(9); 1653-1662; doi: 10.1007/BF01322539

The nucleotide sequence of asinine herpesvirus 3 glycoprotein G indicates that the donkey virus is closely related to equine herpesvirus 1.

Abstract: The nucleotide sequence of the glycoprotein G (gG) homologue of asinine herpesvirus 3 (AHV3), a respiratory alphaherpesvirus of donkeys, was determined. The AHV3 gG gene consists of 1233 base pairs (bp) and codes for a predicted protein of 411 amino acids. This is identical in size to the equine herpesvirus 1 (EHV1) gG gene and 6 amino acids longer than the equine herpesvirus 4 (EHV4) gG gene. The predicted amino acid sequence of AHV3 gG has characteristics of a class 1 membrane protein. The amino acid sequence of AHV3 gG shows 92% and 60% identity to EHV1 gG and EHV4 gG respectively. Two regions within the gG amino acid sequences of EHV1 and EHV4 were previously defined, an N-terminal constant region and an immunodominant highly variable region located toward the C-terminus. In the corresponding constant region of AHV3 gG there was 96% and 75% amino acid identity with EHV1 and EHV4 gGs respectively. In the variable region, there was 73% and 24% identity respectively. Phylogenetic analyses using the gG nucleotide sequences indicated that AHV3 is much closer in evolutionary distance to EHV1 than either virus is to EHV4. These findings provide additional support for the view that AHV3, or another closely related virus, may be the progenitor of EHV1 and has adapted to horses in relatively recent times.
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Publication Date: 1995-01-01 PubMed ID: 7487497DOI: 10.1007/BF01322539Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research reveals that the asinine herpesvirus 3 (AHV3), a virus that affects donkeys’ respiratory systems, is closely related to the equine herpesvirus 1 (EHV1) virus, based on the nucleotide sequence of the glycoprotein (gG) in AHV3.

Understanding the Asinine Herpesvirus 3

  • The research centers on the decoding of the nucleotide sequence of the glycoprotein G homologue in AHV3. This virus affects the respiratory system in donkeys. Through the study, the researchers detected that the AHV3 gG gene comprises 1233 base pairs (nucleotide units) and predicts a protein of 411 amino acids.
  • This predicted protein size is, interestingly, the same size as that in the EHV1 gG gene, and 6 amino acids larger than the EHV4 gG gene.
  • The amino acid sequence in the AHV3 gG reflects characteristics typical of a class 1 membrane protein, providing further insight into the virus’s structure.

Comparing AHV3 to EHV1 and EHV4

  • Looking closely at the AHV3 gG’s amino acid sequence, there is significant identity to both EHV1 and EHV4’s gGs—92% identical to EHV1 and 60% identical to EHV4.
  • Two regions of the EHV1 and EHV4’s gG amino acids sequences have previously been defined as a constant region (N-terminal) and a highly variable immunodominant region (located towards the C-terminus). Matching these regions in the AHV3 gG, researchers found a particularly high identity in the constant region with EHV1 (96%) and EHV4 (75%). In the variable region, a noticeable decrease in identity was observed (73% with EHV1 and just 24% with EHV4).

Insights into Virus Evolution

  • Using the gG nucleotide sequences, phylogenetic analyses were conducted (a study of how different groups of organisms are relationally connected). These analyses showed that the AHV3 virus is much closer in evolutionary terms to EHV1 than either virus is to EHV4.
  • This discovery provides additional support for the theory that AHV3, or a virus closely related, could be the progenitor (originator) of EHV1. This suggests that the virus could have adapted to horses in relatively recent times, tracing an interesting path in the virus’s evolutionary journey.

Cite This Article

APA
Ficorilli N, Studdert MJ, Crabb BS. (1995). The nucleotide sequence of asinine herpesvirus 3 glycoprotein G indicates that the donkey virus is closely related to equine herpesvirus 1. Arch Virol, 140(9), 1653-1662. https://doi.org/10.1007/BF01322539

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 140
Issue: 9
Pages: 1653-1662

Researcher Affiliations

Ficorilli, N
  • Centre for Equine Virology, School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Studdert, M J
    Crabb, B S

      MeSH Terms

      • Amino Acid Sequence
      • Animals
      • Cloning, Molecular
      • Equidae / microbiology
      • Herpesvirus 1, Equid / genetics
      • Horses / microbiology
      • Molecular Sequence Data
      • Phylogeny
      • Sequence Alignment
      • Sequence Homology, Amino Acid
      • Solubility
      • Viral Envelope Proteins / chemistry
      • Viral Envelope Proteins / genetics

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      Citations

      This article has been cited 7 times.
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